Volume-9 , Issue-2 , Aug 2022, ISSN 2349-3178 (Print), 2349-3186 (Online) Go Back

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  Terpenoid Rich Concentrate of Phyllantus amarus (Schum Thonn) Whole Plant’s Potential in High Salt Steered Immunological Storm, Antioxidant and Blood Enzymes Derangement

  Tope Israel Fasan, Olubukola Sinbad Olorunnisola, Adewale Adetutu, Bamidele Stephen Ajilore, Cyril Ohikhatemen Ahonsi

  Research Paper | Journal-Paper (IJMSRP)

  Vol.9 , Issue.2 , pp.1-9, Aug-2022

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  Abstract
  High salt diet driven immunological storm and blood enzymes dissymmetry are established global health challenges, deleteriously inflicting intracellular organelles and related inflammatory cytokines out of homeostatic threshold. Statistic proclaimed by World Health Organization (2012) also reveals the preponderance of about a quarter of the world’s global health linked oxidative damages to high salt-related influences. The aim of the study was to evaluate the serum enzymes’ protective effect, antioxidant and immuno-boosting efficacy of qualitatively affirmed terpenoid rich-concentrate (TRC) of Phyllanthus amarus (Schum and Thonn) whole plant against Dawley rats fed with 8% salty chow (HSD) for 56days. Healthy male rats (n=40) were grouped as follows: Group 1: received only normal rat chow as control group; Group 2: Administered 8% high salt diet (HSD) only; Group 3: co-administered HSD+80mg/kg/bwt., of TRC; Group 4: HSD + 160mg/kg/bwt., of TRC; Group 5: HSD + 200mg/kg/bwt., of TRC. The results (p<0.05) indicated that TRC has a lethal dose (LD50) greater than 5000 mg/kg /b.wt, with a significant increase in the levels of modulating cytokines [interleukin 8 (IL-8), interleukin 6 (IL-6), interleukin 2 (IL-2), and Tumor necrosis factor-alpha (TNF?)], serum enzymes (AST, ALP, CK and LDH), consequent significant reduction in enzymatic antioxidant (GSH, Cat, SOD), but with measurable up-regulation of radical product, MDA, in the serum of rats fed HSD. Though, the deranged parameters of interest were dose-dependently remediated to near normal coefficient in the rodents co-administered with the rich concentrates. It’s of interest that, the treated groups also expressed signs/physical improvement via; hair integrity, health status, and erection of fur to near normal. The results suggested that TRC contains bioactive compounds which could remediate HSD induced immunological storm, blood enzymes derangement and also complement orthodox medicine in the therapeutic search for oxidative related diseases triggered by high salt meals.

  Key-Words / Index Term
  Blood antioxidant enzymes; Phyllanthus amarus (Schum and Thonn); Terpenoid rich concentrate; Immunological Cytokines; Aspartate transaminase; and lethal dose.

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  Citation
  Tope Israel Fasan, Olubukola Sinbad Olorunnisola, Adewale Adetutu, Bamidele Stephen Ajilore, Cyril Ohikhatemen Ahonsi, "Terpenoid Rich Concentrate of Phyllantus amarus (Schum Thonn) Whole Plant’s Potential in High Salt Steered Immunological Storm, Antioxidant and Blood Enzymes Derangement," International Journal of Medical Science Research and Practice, Vol.9, Issue.2, pp.1-9, 2022

• Open Access   Article

  Computation Studies on potential anti-COVID-19 natural compound against The Omicron Variant of SARS-COV-2

  I.V. Ferrari, G. Giuntoli, A. Cavallo, I. Foffa, G. Soldani, P. Losi

  Research Paper | Journal-Paper (IJMSRP)

  Vol.9 , Issue.2 , pp.10-15, Aug-2022

  Abstract

  Full-Text HTML

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  Citation

  Abstract
  For the first time, this short communication aims to carry out the possible antiviral role of a natural compound called Adapalene. This substance has been shown, by the Molecular Simulation method, to be particularly active both in the original SARS-COV-2 protein and in the mutated form Spike protein of SARS-CoV- 2 Omicron (B.1.1.529). The best results obtained have reported excellent Binding Energies Scores values of about -10.35 kcal/mol with an estimated Ki of about 26 nMolar, when Adapalene interacts in the binding active zone of the receptor binding domain (RBD) of SARS-COV-2, with the Human ACE2. Furthermore, this compound can interacts also with other antiviral Coronavirus proteins. Indeed it can bind with other SARS-COV-2 proteins such as SARS-COV-2- 3Cl protease, with an Estimated Binding Energy of approximately, ca -10.24 kcal/mol and an estimated Ki of ca 31.15 nMolar, while with Nucleocapsid Phosphoprotein SARS-COV-2, with a Binding Energy of approximately of ca -10.47kcal / mol and an estimate Ki of ca 21.06 nMolar.

  Key-Words / Index Term
  Covid-19, Adapalene , Docking analysis, SARS-COV-2

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  Citation
  I.V. Ferrari, G. Giuntoli, A. Cavallo, I. Foffa, G. Soldani, P. Losi, "Computation Studies on potential anti-COVID-19 natural compound against The Omicron Variant of SARS-COV-2," International Journal of Medical Science Research and Practice, Vol.9, Issue.2, pp.10-15, 2022