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Purification and Electrophoretic analysis of Extracellular Alkaline Phosphatase from bulb extracts of Urginea species

Banani Misra1 , M.N. Shiva Kameshwari2

  1. Department of Botany, Bangalore University, Jnanabharathi Campus, Bengaluru, India.
  2. Department of Botany, Bangalore University, Jnanabharathi Campus, Bengaluru, India.

Correspondence should be addressed to: bananimisra561@gmail.com.


Section:Research Paper, Product Type: Isroset-Journal
Vol.5 , Issue.2 , pp.38-45, Apr-2018


CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v5i2.3845


Online published on Apr 30, 2018


Copyright © Banani Misra, M.N. Shiva Kameshwari . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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IEEE Style Citation: Banani Misra, M.N. Shiva Kameshwari, “Purification and Electrophoretic analysis of Extracellular Alkaline Phosphatase from bulb extracts of Urginea species,” International Journal of Scientific Research in Biological Sciences, Vol.5, Issue.2, pp.38-45, 2018.

MLA Style Citation: Banani Misra, M.N. Shiva Kameshwari "Purification and Electrophoretic analysis of Extracellular Alkaline Phosphatase from bulb extracts of Urginea species." International Journal of Scientific Research in Biological Sciences 5.2 (2018): 38-45.

APA Style Citation: Banani Misra, M.N. Shiva Kameshwari, (2018). Purification and Electrophoretic analysis of Extracellular Alkaline Phosphatase from bulb extracts of Urginea species. International Journal of Scientific Research in Biological Sciences, 5(2), 38-45.

BibTex Style Citation:
@article{Misra_2018,
author = {Banani Misra, M.N. Shiva Kameshwari},
title = {Purification and Electrophoretic analysis of Extracellular Alkaline Phosphatase from bulb extracts of Urginea species},
journal = {International Journal of Scientific Research in Biological Sciences},
issue_date = {4 2018},
volume = {5},
Issue = {2},
month = {4},
year = {2018},
issn = {2347-2693},
pages = {38-45},
url = {https://www.isroset.org/journal/IJSRBS/full_paper_view.php?paper_id=596},
doi = {https://doi.org/10.26438/ijcse/v5i2.3845}
publisher = {IJCSE, Indore, INDIA},
}

RIS Style Citation:
TY - JOUR
DO = {https://doi.org/10.26438/ijcse/v5i2.3845}
UR - https://www.isroset.org/journal/IJSRBS/full_paper_view.php?paper_id=596
TI - Purification and Electrophoretic analysis of Extracellular Alkaline Phosphatase from bulb extracts of Urginea species
T2 - International Journal of Scientific Research in Biological Sciences
AU - Banani Misra, M.N. Shiva Kameshwari
PY - 2018
DA - 2018/04/30
PB - IJCSE, Indore, INDIA
SP - 38-45
IS - 2
VL - 5
SN - 2347-2693
ER -

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Abstract :
Alkaline phosphatase was purified from three species of Urginea collected from different regions of India, Urginea wightii (Yediyur), Urginea indica(Karwar) and Urginea polyphylla (Castle rock Poona). The procedure followed for purification were Salt precipitation, Dialysis, ion exchange and Gel filtration method. Lowry’s method was used to estimate the Protein concentrations. The purified enzyme was subjected to 12.5% SDS-PAGE for molecular determination of Urginea species. The molecular weight of the protein band obtained was nearly 46 kDa in Urginea indica but Urginea wightii and Urginea polyphylla the protein band obtained was approximately 43kDa. The enzyme activity in these three-species varied. In Urginea indica it was found to be more about 0.152 U/ml while in Urginea wightii showed 0.05U/ml enzyme activity and 0.106 U/ml. in Urginea polyphylla.

Key-Words / Index Term :
Urginea, Alkaline Phosphatase, Purification, SDS-PAGE

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AUTHOR PROFILE
Banani Misra pursed M.Sc. Botany and currently pursuing Ph.D. in Botany from Bengaluru University, Bengaluru, India. She has published 3 research papers in reputed international journals. Her main research work focuses on Biochemical and Enzyme studies. She has 2+ years of teaching experience and 3+ years of research experience.


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