Exploring the Role of Selected Bacterial Strains in Chromium (Cr(VI) Reduction from Soil

Rachna Singh¹ , Mahendra K. Gupta²

Section:Research Paper, Product Type: Isroset-Journal
Vol.6 , Issue.1 , pp.226-232, Feb-2019

CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v6i1.226232

Online published on Feb 28, 2019

Copyright © Rachna Singh , Mahendra K. Gupta . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
World’s rapidly evolving population and its progressive attraction towards luxurious life has unsurprisingly led to an increase in anthropogenic sources of pollution. Environmental contamination of hexavalent chromium Cr(VI) is of serious concern for its toxicity as well as mutagenic and carcinogenic effects. Bacterial chromate reduction is a cost-effective technology for detoxification and removal of Cr(VI) from polluted environment. In current study, a total of 15 Cr(VI) resistant bacterial isolates were isolated after enrichment at three different Cr(VI) concentrations- 25mg/L, 50 mg/L and 100 mg/L. Out of 15 only three- S1B, S10D and S3-A with a maximum tolerance of 100mg/L Cr(VI) concentration were selected for further study. On the basis of biochemical characterization isolates were identified, S1-B as Enterococcus faecalis, S10-D as Bacillus megaterium and S3-A as Bacillus cereus. Efficiency of chromium reduction by selected isolates was evaluated at optimized growth conditions. All the three isolates have shown excellent chromium reduction with 100% by S10D, 78.6 % by S1B and 61.36% by S3-A at 25mg/L of Cr(VI) concentration. All three isolates had shown around 60% reduction within 48 h of incubation period. Multimetal resistance was also determined against six different heavy metals (Hg, Cd, Pb, Zn, Co, Mn). Cd and Hg were highly toxic and supressed the bacterial growth completely, only S10D was resistant to Cd. S2D, S3A and S10D were resistant against Co, Pb and Mn while growth of S3A was inhibited by Zn. These potential bacterial isolates could be used at industrial level for effluent treatment and also have great significance for bioremediation of chromium contaminated sites.

Key-Words / Index Term :
Anthropogenic, bioremediation, carcinogenic, degradation potential, multimetal resistance

References :
[1]. B.L. Martins, C.C.V. Cruz, A.S. Luna, C.A. Henriques, “Sorption and desorption of Pb +2 ions by dead Sargassum sp. Biomass”, Biochemical Engineering Journal, Vol. 27, pp. 310-314, 2006.
[2]. J. Chung, R. Nerenberg, B.E. Rittmann, “Bioreduction of soluble chromate using hydrogen based membrane bioflim reactor”. Water Res. Vol. 40, pp. 1634–1642, 2006.
[3]. EPA, wastewater technology sheet: chemical precipitation. United State Environmental Protection, EPA 832F00018, 2000.
[4]. M.J. Horsfall, F. Ogban, E.E. Akporhonor, ”Sorption of Chromium (VI) from Aqueous Solution by Cassava (Manihot Sculenta CRANZ) Waste Biomass”. Chemistry and Biodiversity, Vol. 3, pp. 161-173, 2006.
[5]. T. Srinath, T. Verma, P.W. Ramteke, S.K. Garg,“Chromium (VI) biosorption and bioaccumulation by chromate resistant bacteria”. Chemosphere, Vol. 48, pp. 427-435, 2002.
[6]. R. Branco, A.P. Chung, A. Verissimo, P.V. Morais, “Impact of chromium-contaminated wastewaters on the microbial community of a river”. FEMS Microbiol. Ecol. Vol. 54, pp. 35–46, 2005.
[7]. APHA, AWWA, WEF, “Standard methods for the examination of water and waste water. 20th American Public Health Association, American Water Works Association”, Water Environment Federation. 1992.
[8]. T.N. Joutey, W. Bahafid, H. Sayel, E.S. Abed, E.N. Ghachtouli, “Remediation of hexavalent chromium by consortia of indigenous bacteria from tannery waste-contaminated biotopes in Fez, Morocco”. International Journal of Environmental Studies, Vol. 68, Issue., 6, pp. 901-912, 2011.
[9]. S.C. Paul, P. Jain, J. Mitra, S. Dutta, P. Bhattacharya, B. Bal, D. Bhattacharya, S.D. Gupta, S. Pal, “Induction of Cr (VI) reduction activity in an Anoxybacillus strain under heat stress: a biochemical and proteomic study”. FEMS Microbiol Lett, Vol. 331, pp. 70–80, 2012.
[10]. A. Malik, R. Jaiswal, “Metal resistance in Pseudomonas strains isolated from soil treated with industrial wastewater”, Journal of Microbiology & Biotechnology, Vol. 16, pp.177-182, 2000.
[11]. J.G. Holt, N.R. Krieg, P.H.A. Sneath, J.T. Staley, S.T. Williams, “Bergey’s Manual of determinative Bacteriology”. 9th ed. Lippioncott Williams and Wilkins, Baltimore, London, pp. 204-209, 2000.
[12]. B.H. Joshi, K.G. Modi, “Screening and characterization of heavy metal resistant bacteria for its prospects in bioremediation of contaminated soil”, Journal of Environmental Research And Development, Vol. 7, Issue., 4A, pp. 1531-1538, 2013.
[13]. Y.G. Liu, W.H. Xu, G.M. Zeng, X. Li, H. Gao, “Cr(VI) reduction by Bacillus sp. isolated from chromium landfill”, Process Biochemistry, Vol. 41, pp. 1981–1986, 2006.
[14]. G. Biswas, R. Das, S.K. Kazy, “Chromium bioremediation by Alcaligenes faecalis strain p-2 newly isolated from tannery effluent”, Journal of Environmental Research And Development. Vol. 9, Issue., 3A, pp. 840-848, 2015.
[15]. R. Bakiyaraj, L. Baskaran, L.A. Chidambaram, T. Mahakavi, M. Santhoshkumar. “Bioremediation of Chromium by Bacillus subtilis and Pseudomonas aeruginosa”, Int. J. Curr. Microbiol. App. Sci, Vol. 3, Issue., 9, pp. 715-719, 2014.
[16]. S. Basu,, M. Dasgupta, B. Chakraborty, “Removal of chromium (VI) by Bacillus subtilis isolated from East Calcutta wetlands, West Bengal, India”, Int. J. biosci, biochem and bioinformatics, Vol. 4, Issue., 1, pp. 7-10, 2014.
[17]. S. Dey, A.K. Paul, “Hexavalent chromium reduction by aerobic heterotrophic bacteria indigenous to chromite mine overburden”, Brazilian Journal of Microbiology, Vol. 44, Issue., 1, pp. 307-315, 2013.
[18]. V. Jyostna, G. Anuhya, U. Managamuri, K. Srinuvasulu and S. Poda, “Outcome of Cadmium on Aminergic System and Protective Function of Vitamin-C”, International Journal of Scientific Research in Biological Sciences, Vol.5, Issue., 2, pp. 18-23, 2018.
[19]. U. Thacker, R. Parikh, Y. Shouche, D. Madamwar, “Reduction of chromate by cell-free extract of Brucella sp. Isolated from Cr(VI) contaminated sites”, Bioresource Technology, Vol. 98, pp. 1541–1547, 2007.
[20]. S. Sultan, S. Hasnain, “Chromate reduction capability of a gram positive bacterium isolated from effluent of dying industry”. Bull. Environ. Contam. Toxicol., Vol. 75, pp. 699–706, 2005.
[21]. G. Ozdemir, N. Ceyhan, T. Ozturk, F. Akirmak, T. Cosar, “Biosorption of chromium (VI), cadmium (II) and copper (II) by Pentoea sp. TEM18”, Chem. Eng. J., Vol. 102, pp. 249-253, 2004.