Purification and Electrophoretic analysis of Extracellular Alkaline Phosphatase from bulb extracts of Urginea species

Banani Misra¹ , M.N. Shiva Kameshwari²

1. Department of Botany, Bangalore University, Jnanabharathi Campus, Bengaluru, India.
2. Department of Botany, Bangalore University, Jnanabharathi Campus, Bengaluru, India.

Correspondence should be addressed to: bananimisra561@gmail.com.

Section:Research Paper, Product Type: Isroset-Journal
Vol.5 , Issue.2 , pp.38-45, Apr-2018

CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v5i2.3845

Online published on Apr 30, 2018

Copyright © Banani Misra, M.N. Shiva Kameshwari . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
Alkaline phosphatase was purified from three species of Urginea collected from different regions of India, Urginea wightii (Yediyur), Urginea indica(Karwar) and Urginea polyphylla (Castle rock Poona). The procedure followed for purification were Salt precipitation, Dialysis, ion exchange and Gel filtration method. Lowry’s method was used to estimate the Protein concentrations. The purified enzyme was subjected to 12.5% SDS-PAGE for molecular determination of Urginea species. The molecular weight of the protein band obtained was nearly 46 kDa in Urginea indica but Urginea wightii and Urginea polyphylla the protein band obtained was approximately 43kDa. The enzyme activity in these three-species varied. In Urginea indica it was found to be more about 0.152 U/ml while in Urginea wightii showed 0.05U/ml enzyme activity and 0.106 U/ml. in Urginea polyphylla.

Key-Words / Index Term :
Urginea, Alkaline Phosphatase, Purification, SDS-PAGE

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AUTHOR PROFILE
Banani Misra pursed M.Sc. Botany and currently pursuing Ph.D. in Botany from Bengaluru University, Bengaluru, India. She has published 3 research papers in reputed international journals. Her main research work focuses on Biochemical and Enzyme studies. She has 2+ years of teaching experience and 3+ years of research experience.