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  Binding Characterization Study of the Interaction of an Anticancer Drug Ifosfamide with Bovine Serum Albumin

  T.S. Rajendra Kumar, M. Uma Mahesh

  Research Paper | Isroset-Journal (IJSRCS)

  Vol.5 , Issue.1 , pp.1-6, Apr-2018

  CrossRef-DOI:   https://doi.org/10.26438/ijsrcs/v5i1.16

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  Abstract
  The interaction between Ifosfamide (IFO) and bovine serum albumin (BSA) has been studied. The studies were carried out in a buffer of medium at pH 7.4 using fluorescence spectroscopy, UV-vis spectroscopy, and Viscometric methods. The results of fluorescence quenching and UV-vis absorption spectra experiments indicated the formation of the complex of BSA-IFO. Binding parameters were determined using the Stern-Volmer equation. From fluorescence and UV-vis spectroscopic data, the binding constant between IFO and BSA was calculated to be 4.275 x 103 L mol-1 and 8.173 x 103 L mol-1 respectively. The results of thermodynamic parameters ∆G˚, ∆H˚ and ∆S˚ at different temperature indicate that the electrostatic interactions and also hydrogen bonds play a major role for IFO-BSA association.

  Key-Words / Index Term
  Ifosfamide, BSA, UV-visible, Fluorescence, viscometry

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  Citation
  T.S. Rajendra Kumar, M. Uma Mahesh, "Binding Characterization Study of the Interaction of an Anticancer Drug Ifosfamide with Bovine Serum Albumin," International Journal of Scientific Research in Chemical Sciences, Vol.5, Issue.1, pp.1-6, 2018