Extraction And Characterization of Natural Dye from Beetroot Peels (Beta vulgaris) For Application in Leather Dyeing

Louret Atsenga¹ , alo ² , Paul K. M. Sang³ , Rose Tanui⁴

Section:Research Paper, Product Type: Journal-Paper
Vol.10 , Issue.2 , pp.8-14, Apr-2023

Online published on Apr 30, 2023

Copyright © Louret Atsenga ,alo, Paul K. M. Sang, Rose Tanui . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
Synthetic dyes are known to be toxic, carcinogenic, mutagenic and non-biodegradable. This has led to increased research by scientists across the globe on natural dyes as viable alternatives. This study has utilized extraction of natural dye using a simple aqueous method which is an economical way of obtaining the dye for application in leather dyeing while utilizing the agricultural waste hence value addition. The dye extract`s functional groups were identified using FT-IR analysis, while the concentration of betalain in the dye extract was determined by UV-VIS analysis. Additionally, various tests were conducted to investigate how changes in temperature and pH affect the dye`s stability. Characteristic bands were observed in the FT-IR spectra, specifically at 3291.89 for OH stretching, 2884.99 and 2829.06 for C-H stretching, 1634.38 for C=O stretching, 1565.92 for N-H bend, and 1319.07 for the amine bond. The UV-VIS analysis showed a characteristic peak at 535 nm indicating the presence of the red- violet betacyanin which was responsible for the red-violet colour of the extract observed with the maximum absorbance of 0.3. The findings of this investigation indicated that the dye extract obtained from beetroot peels has potential for commercial use in the dyeing of leather.

Key-Words / Index Term :
Betalain, beetroot peels, FT-IR, UV-VIS, betacyanin, betaxanthin

References :
[1] G. Nagendrappa, "Sir William Henry Perkin: the man and his ‘mauve’," Resonance, vol. 15, no. 9, pp. 779-793, 2010.
[2] K. Prabhu and A. S. Bhute, "Plant based natural dyes and mordants: A Review," J. Nat. Prod. Plant Resour, vol. 2, no. 6, pp. 649-664, 2012.
[3] R. Christie, Colour chemistry. Royal society of chemistry, 2014.
[4] L. Nambela, L. V. Haule, and Q. Mgani, "A review on source, chemistry, green synthesis and application of textile colorants," Journal of Cleaner Production, vol. 246, p. 119036, 2020.
[5] H.-G. Franck and J. W. Stadelhofer, Industrial aromatic chemistry: raw materials· processes· products. Springer Science & Business Media, 2012.
[6] R. Gupta, A. Kushwaha, D. Dave, and N. R. Mahanta, "Waste management in fashion and textile industry: Recent advances and trends, life-cycle assessment, and circular economy," Emerging Trends to Approaching Zero Waste, pp. 215-242, 2022.
[7] R. T. Kapoor, M. Danish, R. S. Singh, M. Rafatullah, and A. K. HPS, "Exploiting microbial biomass in treating azo dyes contaminated wastewater: Mechanism of degradation and factors affecting microbial efficiency," Journal of Water Process Engineering, vol. 43, p. 102255, 2021.
[8] A. Kumar, A. K. Singh, and R. Chandra, "Recent advances in physicochemical and biological approaches for degradation and detoxification of industrial wastewater," Emerging treatment technologies for waste management, pp. 1-28, 2021.
[9] R. Kishor et al., "Ecotoxicological and health concerns of persistent coloring pollutants of textile industry wastewater and treatment approaches for environmental safety," Journal of Environmental Chemical Engineering, vol. 9, no. 2, p. 105012, 2021.
[10] S. Saxena and A. S. M. Raja, "Natural Dyes: Sources, Chemistry, Application and Sustainability Issues," in Roadmap to Sustainable Textiles and Clothing, (Textile Science and Clothing Technology, pp. 37-80, 2014,.
[11] M. Shahid and F. Mohammad, "Perspectives for natural product based agents derived from industrial plants in textile applications–a review," Journal of cleaner production, vol. 57, pp. 2-18, 2013.
[12] S. Aggarwal, "Indian dye yielding plants: Efforts and opportunities," in Natural Resources Forum, 2021, vol. 45, no. 1: Wiley Online Library, pp. 63-86.
[13] B. Neelwarne and S. B. Halagur, "Red beet: an overview," Red Beet Biotechnology, pp. 1-43, 2013.
[14] P. Esquivel, "Betalains," in Handbook on natural pigments in food and beverages: Elsevier, pp. 81-99, 2016 .
[15] T. Esatbeyoglu, A. E. Wagner, V. B. Schini?Kerth, and G. Rimbach, "Betanin—A food colorant with biological activity," Molecular nutrition & food research, vol. 59, no. 1, pp. 36-47, 2015.
[16] D. Strack, T. Vogt, and W. Schliemann, "Recent advances in betalain research," Phytochemistry, vol. 62, no. 3, pp. 247-269, 2003.
[17] N. Ali and R. El-Mohamedy, "Eco-friendly and protective natural dye from red prickly pear (Opuntia Lasiacantha Pfeiffer) plant," Journal of Saudi chemical society, vol. 15, no. 3, pp. 257-261, 2011.
[18] Y. Fu, J. Shi, S.-Y. Xie, T.-Y. Zhang, O. P. Soladoye, and R. E. Aluko, "Red beetroot betalains: Perspectives on extraction, processing, and potential health benefits," Journal of Agricultural and Food Chemistry, vol. 68, no. 42, pp. 11595-11611, 2020.
[19] R. Mohan, N. Geetha, D. H. Jennifer, and V. Sivakumar, "Studies on natural dye (Pelargonidin) extraction from onion peel and application in dyeing of leather," International Journal of Recent Engineering Science, vol. 7, no. 1, pp. 14-22, 2020.
[20] A. Inayat, S. Khan, A. Waheed, and F. Deeba, "Applications of eco friendly natural dyes on leather using different modrants," Proc. Pakistan Acad. Sci, vol. 47, no. 3, pp. 131-135, 2010.
[21] V. Sivakumar, J. L. Anna, J. Vijayeeswarri, and G. Swaminathan, "Ultrasound assisted enhancement in natural dye extraction from beetroot for industrial applications and natural dyeing of leather," Ultrasonics Sonochemistry, vol. 16, no. 6, pp. 782-789, 2009.
[22] P. Velmurugan, J. Shim, S.-K. Seo, and B.-T. Oh, "Extraction of natural dye from Coreopsis tinctoria flower petals for leather dyeing? An eco-friendly approach," Fibers and Polymers, vol. 17, pp. 1875-1883, 2016.
[23] C. Kurinjimalar, N. Usharani, B. Kanimozhi, G. C. Jayakumar, and S. V. Kanth, "Extraction and optimization of natural colorant from Garcinia mangostana Linn peels for leather dyeing," Cleaner Engineering and Technology, vol. 7, p. 100423, 2022.
[24] A. T. Selvi, R. Aravindhan, B. Madhan, and J. R. Rao, "Studies on the application of natural dye extract from Bixa orellana seeds for dyeing and finishing of leather," Industrial crops and products, vol. 43, pp. 84-86, 2013.
[25] A. K. DEB, M. A. A. SHAIKH, M. Z. SULTAN, and M. I. H. RAFI, "Application of lac dye in shoe upper leather dyeing," Revista de Pielarie Incaltaminte, vol. 17, no. 2, p. 97, 2017.
[26] V. Popescu et al., "Green chemistry in the extraction of natural dyes from colored food waste, for dyeing protein textile materials," Polymers, vol. 13, no. 22, p. 3867, 2021.
[27] M. Zin, E. Márki, and S. Bánvölgyi, "Conventional extraction of betalain compounds from beetroot peels with aqueous ethanol solvent," Acta Alimentaria, vol. 49, no. 2, pp. 163-169, 2020.
[28] A. Yaqub, N. Chaudhary, R. A. A. Bhatti, Z. Iqbal, and M. Habib-ul-Haq, "Green extraction and dyeing of silk from Beta vulgaris peel dye with ecofriendly acid mordants," J. Bio. Env. Sci, vol. 13, no. 4, pp. 308-321, 2018.
[29] E. ONEM, G. GULUMSER, and B. OCAK, "Evaluation of Natural Dyeing of Leather with Rubia tinctorum Extract," Research note, pp. 81-87, 2011, doi: 10.5053/ekoloji.2011.8011.
[30] R. Castellar, J. M. Obón, M. Alacid, and J. A. Fernández-López, "Color properties and stability of betacyanins from Opuntia fruits," Journal of Agricultural and Food Chemistry, vol. 51, no. 9, pp. 2772-2776, 2003.
[31] C.-P. S. Hsu, "Infrared spectroscopy," Handbook of instrumental techniques for analytical chemistry, vol. 249, 1997.
[32] I. Sadowska-Bartosz and G. Bartosz, "Biological properties and applications of betalains," Molecules, vol. 26, no. 9, p. 2520, 2021.
[33] T. E. Moussa-Ayoub, S. K. El-Samahy, S. Rohn, and L. W. Kroh, "Flavonols, betacyanins content and antioxidant activity of cactus Opuntia macrorhiza fruits," Food Research International, vol. 44, no. 7, pp. 2169-2174, 2011.
[34] C. Yao, F. Yongming, G. Jianmin, and L. Houkun, "Coloring characteristics of in situ lignin during heat treatment," Wood Science and Technology, vol. 46, pp. 33-40, 2012.
[35] K. M. Herbach, F. C. Stintzing, and R. Carle, "Betalain stability and degradation—structural and chromatic aspects," Journal of food science, vol. 71, no. 4, pp. R41-R50, 2006.
[36] M. A. Guerrero-Rubio, J. Escribano, F. García-Carmona, and F. Gandía-Herrero, "Light emission in betalains: from fluorescent flowers to biotechnological applications," Trends in Plant Science, vol. 25, no. 2, pp. 159-175, 2020.
[37] S. Gokhale and S. Lele, "Betalain content and antioxidant activity of B eta vulgaris: effect of hot air convective drying and storage," Journal of Food Processing and Preservation, vol. 38, no. 1, pp. 585-590, 2014.
[38] R. Jackman and J. Smith, "Natural food colorants," Anthocyanins and Betalains (Springer, New York), pp. 244-309, 1996.
[39] F. Gandía-Herrero, J. Escribano, and F. García-Carmona, "Structural implications on color, fluorescence, and antiradical activity in betalains," Planta, vol. 232, pp. 449-460, 2010.
[40] G. Calogero, A. Bartolotta, G. Di Marco, A. Di Carlo, and F. Bonaccorso, "Vegetable-based dye-sensitized solar cells," Chemical Society Reviews, vol. 44, no. 10, pp. 3244-3294, 2015.
[41] A. Houghton, I. Appelhagen, and C. Martin, "Natural blues: Structure meets function in anthocyanins," Plants, vol. 10, no. 4, p. 726, 2021.
[42] H. M. Azeredo, "Betalains: properties, sources, applications, and stability–a review," International journal of food science & technology, vol. 44, no. 12, pp. 2365-2376, 2009.
[43] A. Gliszczy?ska-?wig?o, H. Szymusiak, and P. Malinowska, "Betanin, the main pigment of red beet: Molecular origin of its exceptionally high free radical-scavenging activity," Food additives and contaminants, vol. 23, no. 11, pp. 1079-1087, 2006.
[44] M. Skalicky, J. Kubes, H. Shokoofeh, M. Tahjib-Ul-Arif, P. Vachova, and V. Hejnak, "Betacyanins and betaxanthins in cultivated varieties of Beta vulgaris L. compared to weed beets," Molecules, vol. 25, no. 22, p. 5395, 2020.
[45] S. Manchali, K. N. C. Murthy, S. Nagaraju, and B. Neelwarne, "Stability of betalain pigments of red beet," Red beet biotechnology: food and pharmaceutical applications, pp. 55-74, 2012.
[46] Y. Qin, Y. Liu, X. Zhang, and J. Liu, "Development of active and intelligent packaging by incorporating betalains from red pitaya (Hylocereus polyrhizus) peel into starch/polyvinyl alcohol films," Food Hydrocolloids, vol. 100, p. 105410, 2020.
[47] R. Kushwaha, V. Kumar, G. Vyas, and J. Kaur, "Optimization of different variable for eco-friendly extraction of betalains and phytochemicals from beetroot pomace," Waste and Biomass Valorization, vol. 9, no. 9, pp. 1485-1494, 2018.
[48] T. García-Cayuela, A. Gómez-Maqueo, D. Guajardo-Flores, J. Welti-Chanes, and M. P. Cano, "Characterization and quantification of individual betalain and phenolic compounds in Mexican and Spanish prickly pear (Opuntia ficus-indica L. Mill) tissues: A comparative study," Journal of Food Composition and Analysis, vol. 76, pp. 1-13, 2019.
[49] S. N. A. Kumar, S. K. Ritesh, G. Sharmila, and C. Muthukumaran, "Extraction optimization and characterization of water soluble red purple pigment from floral bracts of Bougainvillea glabra," Arabian Journal of Chemistry, vol. 10, pp. S2145-S2150, 2017.
[50] G. A. Molina, A. R. Hernández-Martínez, M. Cortez-Valadez, F. García-Hernández, and M. Estevez, "Effects of tetraethyl orthosilicate (TEOS) on the light and temperature stability of a pigment from Beta vulgaris and its potential food industry applications," Molecules, vol. 19, no. 11, pp. 17985-18002, 2014.
[51] A. A.-A. Ahmed Moussa, A. H. Gomaa, and R. E. El-Azabawy, "Valorization Beetroot Waste for Eco-Friendly Extraction of Natural Dye for Textile and Food Applications," Egyptian Journal of Chemistry, vol. 65, no. 8, pp. 725-736, 2022.
[52] V. Rotich, P. Wangila, and J. Cherutoi, "FT-IR Analysis of Beta vulgaris Peels and Pomace Dye Extracts and Surface Analysis of Optimally Dyed-Mordanted Cellulosic Fabrics," Journal of Chemistry, vol. 2022, 2022.
[53] L. Aztatzi-Rugerio, S. Y. Granados-Balbuena, Y. Zainos-Cuapio, E. Ocaranza-Sánchez, and M. Rojas-López, "Analysis of the degradation of betanin obtained from beetroot using Fourier transform infrared spectroscopy," Journal of food science and technology, vol. 56, no. 8, pp. 3677-3686, 2019.
[54] A. B. D. Nandiyanto, R. Oktiani, and R. Ragadhita, "How to read and interpret FTIR spectroscope of organic material," Indonesian Journal of Science and Technology, vol. 4, no. 1, pp. 97-118, 2019.