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• Open Access   Article

  Determination of heavy metals and phytochemical analysis of some selected medicinal plants

  S.K. Gunavathy and H. Benita Sherine

  Research Paper | Isroset-Journal (IJSRBS)

  Vol.6 , Issue.3 , pp.87-96, Jun-2019

  CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v6i3.8796

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  Abstract
  Natural products had been essentially used by many cultures and traditions from thousands of years. Plants synthesize massive varieties of substances called secondary metabolities (Phytocompounds) and accumulated in it. The possible bioactive phytocompounds like alkaloids, flavanoids, and phenolic compounds, steroids and coumarins etc., are possible sources for drug discovery. A study was conducted to analyse the presence of traces of essential and non essential heavy metals in the preferred medicinal plants. AAS is used to examine the presence of heavy metals in the selected medicinal plant. Results obtained in the present study showed that the medicinal herb analysed contain heavy metals (Fe, Cu, Mn, Zn, Ni, Co, Pb, Al, V, Cr, Mo, Hg, As and Cd). The concentration (ppm) of heavy metals in the plant extracts like Plectranthus mollis, Elaeagnus conferta and Grewia tilaefolia leaf extracts were found using atomic absorption spectrophotometer. The qualitative analysis showed that alkaloids were mainly seen in most of the samples of Hydro alcoholic leaf extracts. Total phenol, Flavonoid, Steroids, Alkaloid and Tannins were present in all the three samples extracted by Hydro alcoholic leaf extracts. Total phenol and Alkaloid were seen more in leaf samples, from the judgment of the results with the defined permissible limits, it was accomplished that the levels of heavy metals present in the herb falls in the permissible limits for consumed medicinal herbs. Purpose of this study is to determine heavy metals contents in chosen herbal plants and also to highlight the health concerns related to the presence of toxic levels of heavy metals. The presence of high amount of phytochemical compounds put forward that the Grewia tilaefolia plant has higher medicinal value and can be extensively studied to extract the natural compounds which are beneficial to human beings and that could be commercialized for higher production than using synthetic drugs with side effects.

  Key-Words / Index Term
  phytochemical, Heavy Metal, Atomic Absorption Spectroscopic

  References
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  Citation
  S.K. Gunavathy and H. Benita Sherine, "Determination of heavy metals and phytochemical analysis of some selected medicinal plants," International Journal of Scientific Research in Biological Sciences, Vol.6, Issue.3, pp.87-96, 2019

• Open Access   Article

  Anti-oxidative Potential Survival of E. crassipes Under Oxidative Stress

  Sangya S. Bais, Lawrence K., Priya S. and Gavhale V.

  Research Paper | Isroset-Journal (IJSRBS)

  Vol.6 , Issue.3 , pp.97-104, Jun-2019

  CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v6i3.97104

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  Abstract
  The present study evaluates the kinetic constants of enzymatic assays in crude extracts of roots and shoots of E. crassipes growing in wastewater bodies situated in the trans-Ganges/Yamuna regions of Allahabad. The increased kinetic constants in crude extracts of shoots and roots of E. crassipes showing the ability to protect plants from heavy metal induced oxidative stress by activating multi-defense mechanisms and for better growth in polluted environments. This study demonstrates the phytoremediation potential of E. crassipes for the removal of polluted effluents and their ability to survive under oxidative stress.

  Key-Words / Index Term
  Phytoremediation, kinetic constants, oxidative stress, wastewater, heavy metal, multi-defense mechanisms

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  Citation
  Sangya S. Bais, Lawrence K., Priya S. and Gavhale V., "Anti-oxidative Potential Survival of E. crassipes Under Oxidative Stress," International Journal of Scientific Research in Biological Sciences, Vol.6, Issue.3, pp.97-104, 2019

• Open Access   Article

  Quality evaluation of Coconut Based Snack during storage period

  M. Sivasakthi, Narayanasamy Sangeetha

  Research Paper | Isroset-Journal (IJSRBS)

  Vol.6 , Issue.3 , pp.105-113, Jun-2019

  CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v6i3.105113

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  Abstract
  Natural versatile ingredients packed with potent nutrients such as beet root, carrot, ginger and mint have been selected for the recent study for their variable vast phenolic compounds and other functional properties as osmotic infusions in the form of filtrates. Sliced samples (0.8±0.1mm) of matured coconuts were subjected to the process osmotic dehydration from 0 min to 720 min at room temperature. Then the slices were dried in a hot-air oven at temperature 45-60°C for about 6-7 hours and freeze drying at temperature (-40 to 30°C) for a period of 14-16 hours. Osmotic medium without the infusion of filtrates of functional ingredients serves as the control. The snack samples were packed using Aluminium foil laminated LDPE pouches with infusion of 100% nitrogen gas composition and stored at ambient temperature till analyzing the shelf life features like free fatty acids, peroxide value, total sugars, moisture content and microbial growth and the results were interpreted using SPSS tool t test (p≤0.05). Upon three months of storage study, the products quality remained good, where as slight deterioration of shelf life characteristics seen beyond three months, showed the keeping quality of the snack.

  Key-Words / Index Term
  Osmotic dehydration, coconuts, shelf life properties, impregnation, antioxidants

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  Citation
  M. Sivasakthi, Narayanasamy Sangeetha, "Quality evaluation of Coconut Based Snack during storage period," International Journal of Scientific Research in Biological Sciences, Vol.6, Issue.3, pp.105-113, 2019

• Open Access   Article

  Association of Regulatory Factor X 6 gene (rs339331) polymorphism with Prostate Cancer: A case-control study from South India

  Swarnalatha Daram, Subhadra Poornima, Syeda Zubeda, Akhilesh P, Ayyala V, Srinadh B, Shailaja P, Sandhya A, Ramakrishna D, Q Hasan

  Research Paper | Isroset-Journal (IJSRBS)

  Vol.6 , Issue.3 , pp.114-118, Jun-2019

  CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v6i3.114118

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  Abstract
  Prostate Cancer (PCA) is a heterogeneous disease which exhibits clinical variability, morphological differences and molecular marker diversity. Currently, Prostate specific antigen (PSA) evaluation is the only clinically relevant non-invasive marker for PCA screening. A number of genome wide association studies (GWAS) have identified many susceptibility markers for PCA, one of which is a single nucleotide polymorphism (SNP) rs339331, in Regulatory Factor X 6 (RFX6) gene. The present study evaluated this polymorphism g.16677T>C of RFX6 gene, in PCA, benign prostate hyperplasia (BPH) and controls by PCR using specifically designed primers, followed by restriction digestion. Results show that the frequency of ‘C’ allele is 0.74 in PCA and 0.20 in BPH, which is lower than the frequency in controls, which is 0.35. The ‘C’ allele of RFX6 genotype is significantly associated with PCA (P<0.05). This is the first case-control study from India which shows an association of ‘C’ allele of RFX6 gene g.16677T>C polymorphism with high risk of developing PCA and a potential to use it as a biomarker for identifying men at risk of developing PCA.

  Key-Words / Index Term
  Benign prostate hyperplasia, genome wide association studies, gene polymorphism, prostate specific antigen

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  Citation
  Swarnalatha Daram, Subhadra Poornima, Syeda Zubeda, Akhilesh P, Ayyala V, Srinadh B, Shailaja P, Sandhya A, Ramakrishna D, Q Hasan, "Association of Regulatory Factor X 6 gene (rs339331) polymorphism with Prostate Cancer: A case-control study from South India," International Journal of Scientific Research in Biological Sciences, Vol.6, Issue.3, pp.114-118, 2019

• Open Access   Article

  Length-Weight Relationship of the Toothed Pony Fish, Gazza Minuta (Bloch, 1797) from Tuticorin Waters of India

  S. Puvaneswari and R. Karuppasamy

  Research Paper | Isroset-Journal (IJSRBS)

  Vol.6 , Issue.3 , pp.119-123, Jun-2019

  CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v6i3.119123

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  Abstract
  Length-weight analysis for specimen comprising 494 males, 515 females and indeterminants of Gazza minuta, collected from Tuticorin fish landing centre was made to assess the relationship between the variables The length-weight relationship was estimated using the exponential equation W = aLn for one species of pony fish belonging to family leiognathidae, species Gazza minuta, in the catches of tuticorin landing centre . The correlation co-efficient (“r” values) of male, female and indeterminants were found to be 0.9750 (p<0.001), 0.9800 (p<0.001) and 0.8931(p<0.001) respectively. Length-weight relationship study in G. minuta obeys cube law, particularly in the adults.

  Key-Words / Index Term
  Gazza minuta, indeterminants, exponential equation, Tuticorin fish

  References
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  Citation
  S. Puvaneswari and R. Karuppasamy, "Length-Weight Relationship of the Toothed Pony Fish, Gazza Minuta (Bloch, 1797) from Tuticorin Waters of India," International Journal of Scientific Research in Biological Sciences, Vol.6, Issue.3, pp.119-123, 2019

• Open Access   Article

  Synthesis, Spectral Characterization And Pharmacological Significance of Fe(III) Complex With Schiff Base and Thiocyanate ION as Ligands

  R. Govindharaju, P. Durairaj, N.Muruganantham, V. Mukil Meenakshi, T. Maruthavanan,T.Ramachandramoorthy

  Research Paper | Isroset-Journal (IJSRBS)

  Vol.6 , Issue.3 , pp.124-130, Jun-2019

  CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v6i3.124130

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  Abstract
  A novel Fe(III) complex of Schiff’s base derived from phenylacetylurea condensed with salicylaldehyde (SBPS) and thiocyanate(SCN-) ion was synthesized by using microwave irradiation. Microwave synthesis gives high yield of the complex within a short time. The molecular formula and the probable geometry of the complex have been deduced from elemental analysis, molar conductance, UV, FT-IR, and Far IR spectra. The molar conductance indicates that the Fe(III) complex is a non-electrolyte. FT-IR spectra show that Schiff’s base and thiocyanate ion are coordinated to the metal ion in a monodentate manner. The electronic spectrum and the magnetic moment indicate the geometry of the complex is found to be distorted octahedral. The antimicrobial activities of ligands and their Fe(III) complex were studied against the few microorganisms by agar - well diffusion method. The complex shows moderate activity against the bacteria and enhanced activity against the fungi as compared to the SBPS ligand.

  Key-Words / Index Term
  Fe(III) complex, SBPS, thiocyanate ion, antimicrobial

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  Citation
  R. Govindharaju, P. Durairaj, N.Muruganantham, V. Mukil Meenakshi, T. Maruthavanan,T.Ramachandramoorthy, "Synthesis, Spectral Characterization And Pharmacological Significance of Fe(III) Complex With Schiff Base and Thiocyanate ION as Ligands," International Journal of Scientific Research in Biological Sciences, Vol.6, Issue.3, pp.124-130, 2019

• Open Access   Article

  Occupational Health Hazards of Women Beedi Rollers in Nippani Taluk, South India

  Kalpana. I. Pattan and Sridevi I. Puranik

  Research Paper | Isroset-Journal (IJSRBS)

  Vol.6 , Issue.3 , pp.130-132, Jun-2019

  CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v6i3.130132

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  Abstract
  In this study, we surveyed 47 women beedi workers of Nippani, a place in North Karnataka of India - known for tobacco trade and industries. We found that this labor intensive task has led to enormous health conditions in the women involved in rolling the beedis manually. Respiratory problems like cough and throat burns were reported by more than 50% of women. The incidence of Orthopedic and Ophthalmic illness was also common. Other health conditions included GI problems, Giddiness and Headache.

  Key-Words / Index Term
  Beedi-workers, Tobacco, Respiratory problems, orthopedic problems, Indian small scale industry

  References
  [1] Srinivasulu K (1997) Impact of liberalization on beedi workers. Econ Polit Wkly 32(11):515-517.
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  Citation
  Kalpana. I. Pattan and Sridevi I. Puranik, "Occupational Health Hazards of Women Beedi Rollers in Nippani Taluk, South India," International Journal of Scientific Research in Biological Sciences, Vol.6, Issue.3, pp.130-132, 2019

• Open Access   Article

  Managing of Collar Rot Disease in Groundnut (Arachis hypogaea L.) by few Chemicals

  Suman Lora, Tahira Begum

  Research Paper | Isroset-Journal (IJSRBS)

  Vol.6 , Issue.3 , pp.133-136, Jun-2019

  CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v6i3.133136

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  Abstract
  Groundnut (Arachis hypogaea L.) is an economically important edible oil seed crop of the world. The groundnut is affected by various pathogens like viruses, bacteria, fungi and nematodes. The collar rot disease of groundnut is caused by Aspergillus niger which is influenced by kharif crop production in some areas of Rajasthan. Different concentrations of few fungicides such as Mancozeb, Carbendazim, Antracol, Kemoxyl , Thiram,Captan and Hexaconazole are inhibited the mycelial growth of fungi. While Mancozeb and Carbendazim were found to effect the maximum mycelial growth of Aspergillus niger. In the present investigation antifungal activity of few chemicals was assayed in vitro by inhibition zone technique for Aspergillus niger.

  Key-Words / Index Term
  Groundnut, Aspergillus niger, managing chemicals (Mancozeb,Carbendazim, Antracol, Kemoxyle , Thiram,Captan and Hexaconazole)

  References
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  Citation
  Suman Lora, Tahira Begum, "Managing of Collar Rot Disease in Groundnut (Arachis hypogaea L.) by few Chemicals," International Journal of Scientific Research in Biological Sciences, Vol.6, Issue.3, pp.133-136, 2019

• Open Access   Article

  Antifungal Activity of leaf extracts of Nerium indicum against Pathogenic Fungi Aspergillus spp.

  Renu Jangid, Tahira begum

  Research Paper | Isroset-Journal (IJSRBS)

  Vol.6 , Issue.3 , pp.137-140, Jun-2019

  CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v6i3.137140

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  Abstract
  Medicinal plants represent a rich source of antimycotic agents. Plant extract has been used traditionally to treat a number of infectious diseases. Medicinal plant Nerium indicum belongs to the family Apocynaceae. The leaf extracts of Nerium indicum exhibited significant antifungal activity against pathogenic fungi (A. niger, A. flavus and A. fumigatus). Aspergillus species were isolated from soil samples. The soil samples collected from Ajmer, Rajasthan (India). Antifungal activities of the different leaf extracts were compared with the zones of inhibition produced by the standard antibiotic (Gresiofulvin). The ethanol, aqueous and DMSO extracts of Nerium indicum showed high antifungal activity against Aspergillus species except ethanol extract show lowest activity against A. niger. These results support the leaf extract of Nerium indicum can be used to cure fungal infections in both plant and human beings.

  Key-Words / Index Term
  Plant extracts, Disc diffusion, Antifungal activity, Aspergillus species

  References
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  Citation
  Renu Jangid, Tahira begum, "Antifungal Activity of leaf extracts of Nerium indicum against Pathogenic Fungi Aspergillus spp.," International Journal of Scientific Research in Biological Sciences, Vol.6, Issue.3, pp.137-140, 2019