Alteration in Antioxidant Defence System and Oxidative Stress in blood of Liver Cirrhosis

A.P. Thakkar¹ , H.J. Shah² , N.D. Patel³

Section:Research Paper, Product Type: Isroset-Journal
Vol.6 , Issue.1 , pp.47-54, Feb-2019

CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v6i1.4754

Online published on Feb 28, 2019

Copyright © A.P. Thakkar, H.J. Shah, N.D. Patel . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
Serum liver marker enzymes ALT, AST, ALP and GGT are generally used as liver marker enzymes however they cannot provide exact idea regarding establishment of liver disease condition because many liver cirrhotic participants indicate non-significant alteration. Present study is aimed to know the level of antioxidant defense system and oxidative stress in controls and cirrhotic participants. The cross-sectional study was carried out at tertiary centre of gastroenterologist unit at Anand. Experimental participants were divided into two groups: Control (n= 52, Age- 26.7±1.23 years, 39 M/ 13 F) and cirrhosis (n= 44, Age- 53.48±2.02 years, 34 M/10 F). All participants underwent anthropometric assessment, clinical examination, biochemical parameters, antioxidant parameters and lipid peroxidation marker or oxidative stress marker. Results of our study indicates non- conclusive increase in serum enzyme level. As an average only 42-67 % of the participants indicates increase (ALT (55%), AST (67.5%), GGT (57.5%) and ALP (42.5%)). The total antioxidant activity (Gallic acid equivalent (GAE (p<0.0001)) and Trolox equivalent (TE) (p<0.0001)) and antioxidant enzymes such as Catalase (CAT (p<0.0001)), Glutathione Peroxidase (GSHPx (p<0.0001)) and Glutathione-s-transferase (GST (p<0.0001)) were significantly decreased except superoxide dismutase (SOD (p<0.05)) during cirrhosis condition. Lipid peroxidation marker or oxidative stress marker (MDA) was significantly increased (p<0.0001) in cirrhotic participants compared to controls. The alteration in the above parameters is among all (100%) the participants. Study strongly indicates that antioxidant enzymes namely GST, GSHPx and CAT can be used as surrogate markers of oxidative stress for the cirrhosis condition.

Key-Words / Index Term :
Anthropometric Assessment; Biochemical Parameters; Total Antioxidant Activity; Antioxidant Enzymes; Oxidative Stress Marker

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