Mode of p38 and Nf-kβ Signalling Pathway on Induction of Apoptosis in Response to Flavonoids of Aloe Vera in Glioblastoma

Ashok Kumar¹ , Anupama Mahajan² , Zarina Begum³

1. Department of Biotechnology, Punjab Technical University, Jalandhar, Kapurthala. India.
2. Department of Biotechnology, Saheed Udham Singh College of Engineering & Technology, Mohali (PB), India.
3. Department of Biotechnology, Saheed Udham Singh College of Engineering & Technology, Mohali (PB), India.

Correspondence should be addressed to: ashoka.kumar2007@rediff.com.

Section:Research Paper, Product Type: Journal-Paper
Vol.6 , Issue.1 , pp.128-134, Feb-2019

CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v6i1.128134

Online published on Feb 28, 2019

Copyright © Ashok Kumar, Anupama Mahajan, Zarina Begum . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
The phytochemical, Flavonoid has been shown apoptosis by numerous studies of cancer cell lines. Here, we have investigated the role of flavonoids of Aloe vera in regulation of apoptosis through p38 and Nf-kβ gene against human glioma-U87MG cells in vitro. The potential invasion of flavonoid on human glioma-U-87MG cells was examined by MTT assay and DNA fragmentation assay and expression of p38 and Nf-kβ gene was analysed by RT-PCR. Flavonoids significantly suppressed the apoptosis with IC50 113.29±0.5µg/ml and confirmed by DNA fragmentation assay. The results suggest that flavonoids induce apoptosis with decreased expression of p38 and Nf-kβ gene and protein analysis with SDS-PAGE. Assessments of cell viability represent that U-87MG cells were very sensitive to flavonoids and indicating DNA and protein synthesis delayed. In conclusion, flavonoids of Aloe showed inhibitory effect on Glioblastoma a brain tumor cells by apoptosis through regulation of p38 and Nf-kβ pathway at transcription and post –transcription level.

Key-Words / Index Term :
Glioblastoma, Aloe vera, Flavonoids, p38, Nf-kβ, U-87MG cell lines, RT-PCR, SDS-PAGE.

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