Identification and Characterization of Bioactive Compounds of Leaves of Justicia Gendarussa Burm. F.

G. Shamili¹ , G. Santhi²

Section:Research Paper, Product Type: Isroset-Journal
Vol.6 , Issue.1 , pp.145-153, Feb-2019

CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v6i1.145153

Online published on Feb 28, 2019

Copyright © G. Shamili , G. Santhi . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
The present investigation has been carried out to find the phytochemicals present in Justicia gendarussa leaves extract. The leaves of Justicia gendarussa were collected from Kadukaval in January 2018 Thanjavur, Tamil Nadu, India. The powder leaf was extracted with aqueous and 70% methanol for 24 hours and studied for FTIR, HPLC and NMR. A preliminary phytochemical testing of the leaves extract where than to identify the phytoconstiuents, which reveals that presence of tannin, saponin, flavonoids, steroids, terpenoids, triterpenoid, carbohydrate, anthroquinone, polyphenol, glycoside bioactive compound was isolated by column chromatography technique. The collected flavonoid fractions was purified by thin layer chromatography. FTIR, HPLC and NMR studies were carried out to find the structure of bioactive compound Quercetin. 1H- NMR and 13C – NMR that reveals the structure of flavonoids. The compound was identified as 3, 3’, 4’, 5, 7 – pentahydroxyflavanone by 1H- NMR and 13C – NMR. All these data obtained in the present investigation supported the rich source of phytochemicals preent in J.gendarussa leaves extract and therby traditional claim associated with J. gendarussa literature.

Key-Words / Index Term :
Justicia gendarussa, phytochemical, FTIR, HPLC and NMR

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