Acute toxicity and ethological responses of oligochaete worm Tubifex tubifex (Muller) exposed to a cationic surfactant Cetylpyridinium chloride

Ritwick Bhattacharya¹ , Arnab Chatterjee² , Nimai Chandra Saha³

Section:Research Paper, Product Type: Isroset-Journal
Vol.6 , Issue.2 , pp.8-14, Apr-2019

CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v6i2.814

Online published on Apr 30, 2019

Copyright © Ritwick Bhattacharya, Arnab Chatterjee, Nimai Chandra Saha . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
Cetylpyridinium chloride is a quaternary cationic surfactant which exhibit broad spectrum antimicrobial activity. The present study was conducted to evaluate the acute toxicity of Cetylpyridinium chloride to a benthic oligochaete worm Tubifex tubifex in terms of LC50 and behavioral responses. All experiments were conducted, in a static bioassay system, using increasing concentrations of Cetylpyridinium chloride for 96 h duration. The LC50 values associated with 95% lower and upper confidence limits for the surfactant were determined statistically using Finney’s Probit Analysis Method developed by EPA and were found to be 0.330 (0.305-0.357) ppm, 0.305 (0.280-0.332) ppm, 0.225 (0.204-0.248) ppm and 0.213 (0.190-0.240) ppm at 24, 48, 72 and 96 hours exposure respectively. The results of regression analysis indicated that the mortality rate varied significantly (p<0.05) with the increasing concentrations of surfactant. In addition, the aquatic worm exhibited abnormal behavioral responses like hyperactivity, decreased clumping tendency, increased mucous secretion and wrinkling effect on increasing concentrations of toxicant and period of exposure. Thus, these findings can be used as a potential tool for creating awareness among people to limit the misuse of household products containing surfactants.

Key-Words / Index Term :
Acute toxicity, Cetylpyridinium chloride, Tubifex tubifex, behavioral response

References :
[1]. C.L. Yuan, Z.Z. Xu, M.X. Fan, H.Y. Liu, Y.H. Xie, T. Zhu, “Study on characteristics and harm of surfactants”, Journal of chemical and pharmaceutical research, Vol.6, Issue.7, pp.2233-2237, 2014.
[2]. G. McDonnell, A.D. Russell, “Antiseptics and disinfectants: activity, action, and resistance”, Clinical microbiology reviews, Vol.14, Issue.1, pp.227, 2001.
[3]. M. Pardini, F. Varaine, E. Iona, E. Arzumanian, F. Checchi, M.R. Oggioni, G. Orefici, L. Fattorini, “Cetyl-pyridinium chloride is useful for isolation of Mycobacterium tuberculosis from sputa subjected to long-term storage”, Journal of clinical microbiology, Vol.43, Issue.1, pp.442-444, 2005.
[4]. X. Costa, E. Laguna, D. Herrera, J. Serrano, B. Alonso, M. Sanz, “Efficacy of a new mouth rinse formulation based on 0.07% cetylpyridinium chloride in the control of plaque and gingivitis: a 6‐month randomized clinical trial”, Journal of clinical periodontology, Vol.40, Issue.11, pp.1007-1015, 2013.
[5]. A.L. Ahmad, S.W. Puasa, “Reactive dyes decolourization from an aqueous solution by combined coagulation/micellar-enhanced ultrafiltration process”, Chemical Engineering Journal, Vol.132, Issue.1-3, pp.257-265, 2007.
[6]. F. Luo, G.M. Zeng, J.H. Huang, C. Zhang, Y.Y. Fang, Y.H. Qu, X. Li, D. Lin, C.F. Zhou, “Effect of groups difference in surfactant on solubilization of aqueous phenol using MEUF”, Journal of hazardous materials, Vol.173, Issue.1-3, pp.455-461, 2010.
[7]. S. Buffet-Bataillon, P. Tattevin, M. Bonnaure-Mallet, A. Jolivet-Gougeon. “Emergence of resistance to antibacterial agents: the role of quaternary ammonium compounds—a critical review”, International journal of antimicrobial agents, Vol.39, Issue.5. pp.381-389, 2012.
[8]. H. Rosen, A. Blumenthal, R. Panasevich, J. McCallum, “Dimethyl sulfoxide (DMSO) as a solvent in acute toxicity determinations”, Proceedings of the Society for Experimental Biology and Medicine, Vol.120, Issue.2, pp.511-514, 1965.
[9]. J.W. Nelson, S.C. Lyster, “The toxicity of myristyl-gamma-picolinium chloride”, Journal of the American Pharmaceutical Association, Vol.35, Issue.3, pp.89-94, 1946.
[10]. P.M. Chapman, “Utility and relevance of aquatic oligochaetes in ecological risk assessment”, In Aquatic Oligochaete Biology VIII, Springer, Dordrecht, pp.149-169, 2001.
[11]. R.J. Aston, “Tubificids and water quality: a review”, Environmental Pollution (1970), Vol.5, Issue.1, pp.1-10, 1973.
[12]. J.L. Kaster, “The reproductive biology of Tubifex tubifex Muller (Annelida: Tubificidae)”, American Midland Naturalist, Vol.104, No.2, pp.364-366, 1980.
[13]. A.S. Chong, R. Hashim, A.B. Ali, “Dietary protein requirements for discus (Symphysodon spp.)”, Aquaculture Nutrition, Vol.6, Issue.4, pp.275-278, 2000.
[14]. C.S. Tamaru, H. Ako, R. Paguirigan, “Essential fatty acid profiles of maturation feeds used in freshwater ornamental fish culture”, Hydrobiologia, Vol.358, Issue.1-3, pp.265-268, 1997.
[15]. M.H. Li, “Survival, mobility, and membrane‐bound enzyme activities of freshwater planarian, Dugesia japonica, exposed to synthetic and natural surfactants”, Environmental toxicology and chemistry, Vol.31, Issue.4, pp.843-850, 2012.
[16]. APHA, “Standard Methods for the Examination of Water and Wastewater 20th Edition”, United Book Press, Maryland, United States, 1998.
[17]. D.J. Finney, “Probit Analysis”, Cambridge University Press, Cambridge, England, pp.333, 1971.
[18]. C. Sarkar, S. Bej, N.C. Saha, “A study on the acute toxicity of Triazophos to Branchiurasowerbyi (Beddard, 1982) and their behavioural changes”, International Journal of Scientific Research, Vol.5, Issue.5, 2016.
[19]. S. Gheorghe, C. Stoica, G.G. Vasile, M. Nita-Lazar, E, Stanescu, I.E. Lucaciu,”Metals Toxic Effects in Aquatic Ecosystems: Modulators of Water Quality”, Water Quality, Hlanganani Tutu, IntechOpen, 2017.
[20]. R.W. Edwards, V.M. Brown, “Pollution and fisheries: a progress report”, Water Pollution Control, Vol.66, pp.63-78, 1967.
[21]. G.E. Burdick, “Use of bioassays in determining levels of toxic wastes harmful to aquatic organisms”, American Fisheries Society Special Publication, Vol.4, pp.7-12, 1967.
[22]. J.B. Sprague, “Measurement of pollutant toxicity to fish—III: Sublethal effects and “safe” concentrations”, Water Research, Vol. 5, Issue.6, pp.245-266, 1971.
[23]. Committee on Water Quality Criteria, “A report of the committee on water quality and research series”, US Environmental Protection Agency Report; Lincinnati, OH, USA, 1972.
[24]. National Academy of Science/National Academy of Engineering (NAS/NAE), “Water quality criteria, EPA –R3-033”, US Government Printing Office: Washington DC, USA, 1973.
[25]. International Joint Commission (IJC), “New and Revised Great Lakes Water Quality Objectives. Great lake basin, Windsor”, IJC: Ottawa, ON, Canada, 1977.
[26]. Canadian Council of Resources and Environmental Ministry, “Canadian Water Quality Guidelines”, Canadian Council of Resources and Environmental Ministry, Inland waters Directorate, Environment Canada, Ottawa, Canada, 1991.
[27]. E.E. Little, S.E. Finger, “Swimming behavior as an indicator of sublethal toxicity in fish”, Environmental Toxicology and Chemistry: An International Journal, Vol.9, Issue.1, pp.13-19, 1990.
[28]. S. Bej, D. Mukherjee, N.C. Saha, “Acute toxicity of alpha-cypermethrin to oligochaete worm, Branchiura sowerbyi (Beddard, 1982) along with their behavioural responses”, International Journal of Scientific Research, Vol.4, Issue.12, 2015.
[29]. K Dhara, D Mukherjee, NC Saha, “Acute Toxicity of Cadmium to Benthic Oligochaete Worm, Branchiura sowerbyi Beddard, 1982 and Juvenile Catfish, Clarias batrachus Linnaeus, 1758”, Proceedings of the Zoological Society, Springer, India, Vol.68, No.2, pp.116-119, 2015.