Production and optimization of Polyhydroxybutyrate and its application in bioplastic production

S.A. Kate¹ , D.R. Hire² , N.M. Pawar³

Section:Research Paper, Product Type: Isroset-Journal
Vol.6 , Issue.2 , pp.50-58, Apr-2019

CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v6i2.5058

Online published on Apr 30, 2019

Copyright © S.A. Kate, D.R. Hire, N.M. Pawar . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

View this paper at   Google Scholar | DPI Digital Library

XML View     PDF Download

How to Cite this Paper

576 Views    253 Downloads    102 Downloads

Abstract :
Polyhydroxybutyrate (PHB) is a natural biopolymer by wide range of microbes which is a storage granule used by microbes as an energy as well as carbon source. PHB exhibit stiffness, brittle to rubber like, biodegradable, environment friendly properties. In present work isolates was produced Polyhydroxybutyrate by using Nitrogen deficient medium and modified LB medium with glucose as carbon source. Maximum production was observed in Modified LB medium as compared to nitrogen deficient medium. Extraction of bacterial PHB was done by sodium hypochlorite method; selected bacteria produced 2.10g/L PHB .Confirmation of PHB molecule was by FTIR & TLC. Extracted bacterial PHB was capable to produce biodegradable plastic which shows water tolerance activity. PHB production was found to be maximum at optimum pH 7.5, 37°C, 3% inoculums percentage and glucose concentration 20g/L.

Key-Words / Index Term :
PHB, Nitrogen deficient medium, Modified LB medium, FTIR, TLC, Biodegradable plastic

References :
[1]. Anish Kumari Bhuwal,1Gulab Singh, Neeraj Kumar Aggarwal, Varsha Goyal& Anita Yadav, “ Isolation and Screening of Polyhyroxyalkanoates Producing Bacteria From Pulp, Paper, and Cardboard Industry Waste”. Hindawi Publishing Corporation International Journal of Biomaterials, Vol. 2013, 752821, pp. 1-10, 2013.
[2]. Abdullah A. Alarfaj, Mohammed Arshad, Essam N. Sholkamy, Murugam A. Munusamy, “Extraction and Characterization of Polyhydroxybutyrate (PHB) from Bacillus thuringiensis KSADL127 Isolated from Mangrove Environments of Saudi Arabia.” Brazilian Archives of Biology and Technology, Vol. 58, Issue., 5, 2015.
[3]. Jehan Mohammed El- Mohamedy Hawas, Tarek El-Said El- Banna, ElsayedBelalAbdelmontelebBelal, et.al., “Production of Bioplastic from some Selected Bacterial Strains.” International Journal of Current Microbiology and Applied Sciences, Vol. 5, Issue., 1, pp. 10-22, 2016.
[4]. Akram Hoceinabadi1, Iraj Rasooli, MojtabaTaran, “Isolation and Identification of Poly β - Hydroxybutyrate Over-Producing Bacteria and Optimization of Producing Medium”. Jundishapur J Microbiol, Vol. 8, Issue., 7: e16965, 2015.
[5]. Neelam K. Naranje, Bharat J. Wadher, Hemant J. Purohit, Manohar G. Muddeshwar, “Bacillus megaterium as potential producer for Polyhydroxybutyrate.” IOSR Journal of Environmental Science, Toxicology and Food Technology, Vol. 1, Issue., 6, pp. 07-10, 2015.
[6]. S. Kumaravel, R. Hema & R. Lakshmi, “Production of Polyhydroxybutyrate (bioplastic) and its Biodegradation by Pseudomonas lemoignei and Aspergillusniger.” E Journal of Chemistry, Vol. 7, Issue.,S1, pp. S536-S542, 2010.
[7]. Gupta Shraddha, Rajput Yogita, Shit Simanta, ShuklaAparna, ShuklaKamlesh, “Screening and Production of bioplastic (PHAs) from Sugarcane Rhizospheric Bacteria.” International Multidisciplinary Research Journal, Vol. 1, Issue., 9, pp. 30-33, 2011.
[8]. B. Senthil Kumar and G Prabhakarn, “Production of PHB (bioplastic) using bio-effluents substrate by Alcaligens eutrophus.” Indian Journal of Biotechnology, Vol. 5, pp. 76-79, 2006.
[9]. Anteneh Getachew and Fantahun Woldesenbet “Production of biodegradable plastic by Polyhydroxybutyrate (PHB) accumulating bacteria using low cost agricultural waste material.” Getachew and Woldesendet BMC Res Notes, Vol. 9, pp. 501-509, 2016.
[10]. Xiao-Ran Jiang, Huan Wang, Guo-Qiang Chen, “Engineering the bacterial shapes for enhanced inclusion bodies accumulation” Metabolic Engineering, Vol. 29, pp. 227-237, 2015.
[11]. Gulab Singh, Anish Kumari, Aparna Mittal, Anita Yadav & Neeraj k. Aggarwal, “Poly β-Hydroxybutyrate Production by Bacillus subtilis NG 220 Using Sugar Industry Waste Water.” Hindawi Publishing Corporation International Journal of Biomaterials, Vol. 2013, 952641, pp. 1-10, 2013.
[12]. Robert Findlay & David C. White, “Polymeric Beta-Hydroxyalkanoates from Environmental Samples and Bacillus megaterium, Applied and Environmental Microbiology, Vol. 45, pp. 71-78, 1983.
[13]. S. Sujatha and D. Ravi, “A study on poly hydroxy butyrate (PHB) production and its application from Pseudomonas species (MTCC).” Biosciences, Biotechnology Research Asia, Vol.7, Issue., 2, pp. 825 -832, 2010.
[14]. Prayashree Bahalia, “Estimation of Kinetic Parameters for Production of PHB”.
[15]. Wala’a Shawkat Ali, Neihaya Heikmat Zaki and Somaya Yassen Najim Obiad “Production of bioplastic by bacteria isolated from local soil and organic wastes.” Current Research in Microbiology and Biotechnology Vol. 5, Issue., 2, pp. 1012-1017, 2017.