Full Paper View Go Back

Isolation and Screening of Chlorella Sorokiniana for Wastewater Treatment and Biodiesel Production

Manjunatha S.S1 , S.T. Girisha2

Section:Research Paper, Product Type: Isroset-Journal
Vol.6 , Issue.2 , pp.59-67, Apr-2019


CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v6i2.5967


Online published on Apr 30, 2019


Copyright © Manjunatha S.S, S.T. Girisha . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

View this paper at   Google Scholar | DPI Digital Library


XML View     PDF Download

How to Cite this Paper

  • IEEE Citation
  • MLA Citation
  • APA Citation
  • BibTex Citation
  • RIS Citation

IEEE Style Citation: Manjunatha S.S, S.T. Girisha, “Isolation and Screening of Chlorella Sorokiniana for Wastewater Treatment and Biodiesel Production,” International Journal of Scientific Research in Biological Sciences, Vol.6, Issue.2, pp.59-67, 2019.

MLA Style Citation: Manjunatha S.S, S.T. Girisha "Isolation and Screening of Chlorella Sorokiniana for Wastewater Treatment and Biodiesel Production." International Journal of Scientific Research in Biological Sciences 6.2 (2019): 59-67.

APA Style Citation: Manjunatha S.S, S.T. Girisha, (2019). Isolation and Screening of Chlorella Sorokiniana for Wastewater Treatment and Biodiesel Production. International Journal of Scientific Research in Biological Sciences, 6(2), 59-67.

BibTex Style Citation:
@article{S.S_2019,
author = {Manjunatha S.S, S.T. Girisha},
title = {Isolation and Screening of Chlorella Sorokiniana for Wastewater Treatment and Biodiesel Production},
journal = {International Journal of Scientific Research in Biological Sciences},
issue_date = {4 2019},
volume = {6},
Issue = {2},
month = {4},
year = {2019},
issn = {2347-2693},
pages = {59-67},
url = {https://www.isroset.org/journal/IJSRBS/full_paper_view.php?paper_id=1277},
doi = {https://doi.org/10.26438/ijcse/v6i2.5967}
publisher = {IJCSE, Indore, INDIA},
}

RIS Style Citation:
TY - JOUR
DO = {https://doi.org/10.26438/ijcse/v6i2.5967}
UR - https://www.isroset.org/journal/IJSRBS/full_paper_view.php?paper_id=1277
TI - Isolation and Screening of Chlorella Sorokiniana for Wastewater Treatment and Biodiesel Production
T2 - International Journal of Scientific Research in Biological Sciences
AU - Manjunatha S.S, S.T. Girisha
PY - 2019
DA - 2019/04/30
PB - IJCSE, Indore, INDIA
SP - 59-67
IS - 2
VL - 6
SN - 2347-2693
ER -

639 Views    335 Downloads    133 Downloads
  
  

Abstract :
Abstract- Microalgae grown on wastewater are a probable source of low cost wastewater treatment and biodiesel production. In the study, microalgae was enumerated and identified as Chlorella sorokiniana by 18S rDNA sequence which was cultivated in different wastewater for nutrient removal as well as biodiesel production were studied. The results reveal that the pH of different wastewater samples almost neutralized by microalgae, the total dissolved solids elimination ranging from 52 to 66%, the biological oxygen demand removal efficiency varied much among different wastewaters the removal rate is of 72 to 90%, the chemical oxygen demand removal ranges from 59 to 75%. The magnesium level was decreased and efficiency is about 36 to 60%, the sulphates absorption efficiency was ranging from 57 to 68% and the chloride removal efficiency was 13 to 33%. The lipid content was obtained from the algal biomass which are grown in different wastewater samples was transesterified for biodiesel production, the biodiesel was analyzed by FTIR which meets the ASTM and EU standards, hence from the current study it is evident that Chlorella sorokiniana can be effectively used for potential source for phycoremediation and biodiesel production.

Key-Words / Index Term :
Chlorella sorokiniana, Wastewater, Biomass, Lipid, Transesterification, Biodiesel

References :
[1] B.M. Gitte, S. Siraj, H.M. Dharmadhikari, “Performance and Emission Characteristics of Diesel Engine Fuelled with Biodiesel and its Blend: A review”, International Journal of Engineering Research & Technology, No.2, pp.3235-3243, 2013.
[2] A. Demirbas, “Biofuels securing the planet’s future energy needs”, Energy conversion and management, Vol.50, No.9, pp.2239-2249, 2009.
[3] J. Yaghoubi, M. Yazdanpanah, N. Komendantova, “Iranian agriculture advisors` perception and intention toward biofuel: Green way toward energy security, rural development and climate change mitigation”, Renewable Energy, Vol.130, pp.452-459, 2019.
[4] D. Huang, H. Zhou, L. Lin, “Biodiesel: an alternative to conventional fuel”, Energy Procedia, Vol.16, pp.1874-1885, 2012.
[5] N. Abdel-Raouf, A.A. Al-Homaidan, I.B.M. Ibraheem, “Microalgae and wastewater treatment”, Saudi journal of biological sciences, Vol.19, No.3, pp.257-275, 2012.
[6] N.F. Gray, “Biology of Wastewater Treatment”, Oxford Univ. Press, Oxford, 1989.
[7] C. Zhao, T. Brück, J.A. Lercher, “Catalytic deoxygenation of microalgae oil to green hydrocarbons”, Green Chemistry, Vol.15, No.7, pp.1720-1739, 2013.
[8] I. Ozkurt, “Qualifying of safflower and algae for energy”, Energy Educ Sci Technol, Vol.23 (part A), No.1, pp.145–151, 2009.
[9] Y. Chisti, “Biodiesel from microalgae”, Biotechnology advances, Vol.25, No.3, pp.294-306, 2007.
[10] Y. Liang, N. Sarkany, Y. Cui, “Biomass and lipid productivities of Chlorella vulgaris under autotrophic, heterotrophic and mixotrophic growth conditions”, Biotechnology letters, Vol.31, No.7, pp.1043-1049, 2009.
[11] W.J. Oswald, “Micro-algae and wastewater treatment”, Microalgal biotechnology, pp.305-328, 1988.
[12] N.F.Y. Tam, Y.S. Wong, “Wastewater nutrient removal by Chlorella pyrenoidosa and Scenedesmus sp”, Environmental Pollution, Vol.58, No.1, pp.19-34, 1989.
[13] U.F. Meeranayak, C.T. Shivasharana “Competitive and Economically Feasible Cell Wall Disruption Techniques for Algal Biofuel Extraction”, International Journal of Scientific Research in Biological Sciences Vol.5, No.6, pp.121-126, 2018.
[14] W. Zhou, B. Hu, Y. Li, M. Min, M. Mohr, Z. Du, R. Ruan, “Mass cultivation of microalgae on animal wastewater: a sequential two-stage cultivation process for energy crop and omega-3-rich animal feed production”, Applied biochemistry and biotechnology, Vol.168, No.2, pp.348-363, 2012.
[15] A.N. Shilton, D.D. Mara, R. Craggs, N. Powell, “Solar-powered aeration and disinfection, anaerobic co-digestion, biological CO2 scrubbing and biofuel production: the energy and carbon management opportunities of waste stabilisation ponds”, Water science and technology, Vol.58, No.1, pp.253-258, 2008.
[16] Q. Hu, M. Sommerfeld, E. Jarvis, M. Ghirardi, M. Posewitz, M. Seibert, A. Darzins, “Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances”, The plant journal, Vol.54, No.4, pp.621-639, 2008.
[17] P.T. Vasudevan, M. Briggs, “Biodiesel production—current state of the art and challenges”, Journal of industrial microbiology & biotechnology, Vol.35, No.5, pp.421, 2008.
[18] J. Janaun, N. Ellis, “Perspectives on biodiesel as a sustainable fuel”, Renewable and Sustainable Energy Reviews, Vol.14, No.4, pp.1312-1320, 2010.
[19] A.B. Fadhil, E.T. Al-Tikrity, M.A. Albadree, “Biodiesel production from mixed non-edible oils, castor seed oil and waste fish oil”, Fuel, Vol.210, pp.721-728, 2017.
[20] S.M. Phang, W.L. Chu, “Algae culture Collection, Catalogue of Strains”, Institute of Post Graduate Studies and Research, University of Malaya, Kuala Lumpur, Malaysia, pp.77, 1999.
[21] L.E. Eland, R. Davenport, C.R. Mota, “Evaluation of DNA extraction methods for freshwater eukaryotic microalgae”, Water research, Vol.46, No.16, pp.5355-5364, 2012.
[22] American Public Health Association, “Standard methods for the examination of water and wastewater”, 1998.
[23] H.Q. Luyen, J.Y. Cho, H.W. Shin, N.G. Park, Y.K. Hong, “Microalgal growth enhancement by levoglucosan isolated from the green seaweed Monostroma nitidum”, Journal of applied phycology, Vol.19, No.2, pp.175-180, 2007.
[24] S.B. Velasquez-Orta, J.G.M. Lee, A. Harvey, “Alkaline in situ transesterification of Chlorella vulgaris”, Fuel, Vol.94, pp.544-550, 2012.
[25] A.Y. Oyerinde, E.I. Bello, “Use of fourier transformation infrared (FTIR) spectroscopy for analysis of functional groups in peanut oil biodiesel and its blends”, British Journal of Applied Science & Technology, Vol.13, No.3, pp.1-14, 2016.
[26] H. Qiao, G. Wang, X. Zhang, “Isolation and characterization of Chlorella Sorokiniana GXNN01 (chlorophyta) with the properties of heterotrophic and microaerobic growth”, Journal of phycology, Vol.45, No.5, pp.1153-1162, 2009.
[27] V.A. Huss, C. Frank, E.C. Hartmann, M. Hirmer, A. Kloboucek, B.M. Seidel, E. Kessler, “Biochemical taxonomy and molecular phylogeny of the genus Chlorella sensu lato (Chlorophyta)”, Journal of Phycology, Vol.35, No.3, pp.587-598, 1999.
[28] S.K. Gupta, F.A. Ansari, A. Shriwastav, N.K. Sahoo, I. Rawat, F. Bux, “Dual role of Chlorella sorokiniana and Scenedesmus obliquus for comprehensive wastewater treatment and biomass production for bio-fuels”, Journal of cleaner production, Vol.115, pp.255-264, 2016.
[29] J.K. Pittman, A.P. Dean, O. Osundeko, “The potential of sustainable algal biofuel production using wastewater resources”, Bioresource technology, Vol.102, No.1, pp.17-25, 2011.
[30] Gupta, L. Prabuddha, Hee-Jeong Choi, R. Radheshyam Pawar, P. Sokhee Jung, Seung-Mok Lee. “Enhanced biomass production through optimization of carbon source and utilization of wastewater as a nutrient source”, Journal of environmental management, Vol.184, pp.585-595, 2016.
[31] S. Huo, Z. Wang, S. Zhu, W. Zhou, R. Dong, Z. Yuan, “Cultivation of Chlorella zofingiensis in bench-scale outdoor ponds by regulation of pH using dairy wastewater in winter, South China” Bioresource technology, Vol.121, pp.76-82, 2012.
[32] G.K. Sharma, S.A. Khan, “Bioremediation of sewage wastewater using selective algae for manure production”, International Journal of Environmental Engineering and Management, Vol.4, No.6, pp.573-580, 2013.
[33] O. Colak, Z. Kaya, “A study on the possibilities of biological wastewater treatment using algae”, Doga Biyolji Serisi, Vol.12, No.1, pp.18–29, 1988.
[34] L. Wang, M. Min, Y. Li, P. Chen, Y. Chen, Y. Liu, R. Ruan, “Cultivation of green algae Chlorella sp. in different wastewaters from municipal wastewater treatment plant”, Applied biochemistry and biotechnology, Vol.162, No.4, pp.1174-1186, 2010.
[35] S. Kim, J.E. Park, Y.B. Cho, S.J. Hwang, “Growth rate, organic carbon and nutrient removal rates of Chlorella sorokiniana in autotrophic, heterotrophic and mixotrophic conditions”, Bioresource technology, Vol.144, pp.8-13, 2013.
[36] S. Mandal, N. Mallick, “Microalga Scenedesmus obliquus as a potential source for biodiesel production”, Applied microbiology and biotechnology, Vol.84, No.2, pp.281-291, 2009.
[37] N.M. Deleebeeck, F. De Laender, V.A. Chepurnov, W. Vyverman, C.R. Janssen, K.A. De Schamphelaere, “A single bioavailability model can accurately predict Ni toxicity to green microalgae in soft and hard surface waters”, Water research, Vol.43, No.7, pp.1935-1947, 2009.
[38] W.J. Oswald, “Waste treatment by pond systems, engineering aspects” In Proc. IAWPRC Conference on Appropriate Waste Management Technologies, Perth, Australia, 1991.
[39] N. Renuka, A. Sood, S.K. Ratha, R. Prasanna, A.S. Ahluwalia, “Nutrient sequestration, biomass production by microalgae and phytoremediation of sewage water”, International journal of phytoremediation, Vol.15, No.8, pp.789-800, 2013.
[40] C.M. Kuo, T.Y. Chen, T.H. Lin, C.Y. Kao, J.T. Lai, J.S. Chang, C.S. Lin, “Cultivation of Chlorella sp. GD using piggery wastewater for biomass and lipid production”, Bioresource technology, Vol.194, pp.326-333, 2015.
[41] X. Li, H. Xu, Q. Wu, “Large‐scale biodiesel production from microalga Chlorella protothecoides through heterotrophic cultivation in bioreactors”, Biotechnology and bioengineering, Vol.98, No.4, pp.764-771, 2007.
[42] H. Wang, H. Xiong, Z. Hui, X. Zeng, “Mixotrophic cultivation of Chlorella pyrenoidosa with diluted primary piggery wastewater to produce lipids”, Bioresource Technology, Vol.104, pp.215-220, 2012.
[43] A. Ranjan, C. Patil, V.S. Moholkar, “Mechanistic assessment of microalgal lipid extraction”, Industrial & Engineering Chemistry Research, Vol.49, No.6, pp.2979-2985. 2010.
[44] T.C. Loong, A. Idris, “Rapid alkali catalyzed transesterification of microalgae lipids to biodiesel using simultaneous cooling and microwave heating and its optimization”, Bioresource technology, Vol.174, pp.311-315, 2014.
[45] P. Nautiyal, K.A. Subramanian, M.G. Dastidar, “Production and characterization of biodiesel from algae”, Fuel Processing Technology, Vol.120, pp.79-88, 2014.

Authorization Required

 

You do not have rights to view the full text article.
Please contact administration for subscription to Journal or individual article.
Mail us at  support@isroset.org or view contact page for more details.

Go to Navigation