Antiapoptotic Effect of Ajuga bracteosa Leaves on Hepatic Dysfunction in Alloxan Induced Diabetic Chicks

R. Bisht¹ , D. Kumar²

Section:Research Paper, Product Type: Isroset-Journal
Vol.6 , Issue.2 , pp.192-196, Apr-2019

CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v6i2.192196

Online published on Apr 30, 2019

Copyright © R. Bisht, D. Kumar . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
The present study was carried out to assess the antiapoptotic effect of Ajuga bracteosa leaves on the hepatic dysfunction in alloxan induced diabetic chicks. Hydroalcoholic extract of Ajuga bracteosa leaves was prepared by standard method as prescribed earlier and apply for monitoring the liver enzymes including Alkaline phosphatase (ALP), Aspartate amino transferase (AST - SGOT) and Alanine amino transferase (ALT - SGPT) in experimental chicks, including diabetic control and diabetic treated with the hydroalcoholic extract of Ajuga bracteosa leaves at the rate of 100gm/Kg body weight and normal control chicks fed with water ad libitum. Present study reveals a significant (p<0.05) decline in the level of these enzymes in the chicks treated with hydroalcoholic extract of Ajuga bracteosa leaves up to the control level as compared to chicks of diabetic control group. Therefore, administration of hydroalcoholic extract of Ajuga bracteosa leaves in diabetic chicks significantly ameliorate the enzymological parameters for the assessment of hepatic dysfunction in diabetic chicks, which showed the potent antiapoptotic effect of Ajuga bracteosa leaves extract on hepatic dysfunction in alloxan induced diabetic chicks.

Key-Words / Index Term :
Antiapoptotic, Ajuga bracteosa, Hepatic, Alloxan, Diabetes, ALP, AST - SGOT, ALT - SGPT

References :
[1]. C.N. Aguwa, “Diabetes mellitus. In: Therapeutic basis of climate pharmacy in the tropics”, Optimal publishers, Enugu, Nigeria. pp.1 – 453, 1994.
[2]. H.U. Nwanjo, E.A. Nwokoro, “Anti-diabetic and biochemical effects of aqueous extract of V. amygdalina leaf in normoglycaemic and diabetic rats”, J. Innov. Life Sci, 7:6 – 10, 2004
[3]. G.N. Levinthal, A.S. Tavili, “Liver disease and diabetes mellitus”, Clin Diabetes, 17:2, 1999.
[4]. C.P. Khare, “Indian Medicinal Plants - An Illustrated Dictionary”, 1st Indian Reprint Springer (India) Pvt. Ltd., New Delhi, India, 28, 2007.
[5]. P.R. Rastogi, B.N. Mehrotra, Central Drug Research. Institute of Sciences communication, New Delhi,1:19, 1960-1966.
[6]. R.N. Chopra, S.L. Nayar, Chopra C, Glossary Indian Medicinal Plants, PID: New Delhi, 10, 1956.
[7]. K.M. Nadkarni, Indian Materia Medica, Popular prakashan private Ltd., Mumbai, 58, 1976.
[8]. Z.H. Israili, B. Lyoussi, “Ethnopharmacology of the plants of genus Ajuga”, Pak. J. Pharm. Sci. 22(4): 425-462, 2009.
[9]. S. Chandel, U. Bagai, “Antiplasmodial activity of Ajuga bracteosa against Plasmodium berghei infected BALB/c mice”, Indian J. Med. Res. 131: 440-444, 2010.
[10]. L.C. Tapsell, I. Hemphill, L. Cobiac, C.S. Patch, D.R. Sullivan, M. Fenech, S. Roodenrys, J.B. Keogh, P.M. Clifton, P.G. Williams, V.A. Fazio, K.E. Inge, “Health benifits of herbs and species: the past, the present, the future”, Med J 185 (4suppl): S4-S24, 2006.
[11]. R.N. Okigbo, E.C. Mmeka, “An appraisal of phytomedicine in Africa”, Sci Technol J, 6:83-94, 2006.
[12]. M.N. Cook, C.J. Girman, P.P. Stein, C.M. Alexander, R.R. Holman, “Glycaemic control continues to deteriorate after sulfonylureas are added to metformin among patients with type 2 diabetes”, Diabetes Care, 28:995-1000, 2005.
[13]. L. Cristina, L. Roberto, D.P. Stefano, “β-cell failure in type 2 diabetes mellitus”, Curr Diab Rep, 8:179-184, 2008.
[14]. M.A. Nagy, S.A. Mohamed, “Antidiabetic effect of Cloem Droserifolia (Cloemaceae)”, American Journal of Biochemistry, 4(4):68-75, 2014.
[15]. P. Daisy, K. Saipriya, “Biochemical analysis of Cassia fistula aqueous extract and Phytochemically synthesized gold nanoparticles as hypoglycemic treatment for diabetes mellitus”, Int J Nanomedicine, 7:1189-1202, 2012.
[16]. H.R. Rosen, E.B. Keefe, “Evaluation of abnormal liver enzymes, use of liver tests and the serology of viral hepatitis: liver disease, diagnosis and management”, New York; Churchill livingstone publishers; 1sted., p. 24-35, 2000.
[17]. S.F. Friedman, P. Martin, J.S. Munoz, “Laboratory evaluation of the patient with liver disease. Hepatology, a textbook of liver disease”, Philedelphia, Saunders publication, 1:661-709, 2003.
[18]. S. Sherlock, “Assessment of liver function disease of liver and biliary system”, London, Blackwell science ltd, 10thed., p. 17-32, 1997.
[19]. N.R. Ratod, I. Raghuveer, H.R. Chitme, R. Chandra, “Free radical scavenging activity of Calotropis gigantea on streptozotocin induced diabetic rats”, Indian J Pharm Sci, 71(6):615-621, 2009.
[20]. C.V. Diogo, J.M. Suski, M. Lebiedzinska, “Cardiac mitochondrial dysfunction during hyperglycemia-the role of oxidative stress and p66Shc signalling”, Int J Biochem Cell Biol, 45:114-22, 2013.
[21]. K. Asayama, F. Nyfeler, D. English, S.J. Pilkis, I.M. Burr, “Alloxan-induced free radical production in isolated cells selective effect on islet cells”, Diabetes, 33:1006-12, 1984.
[22]. I.S. Young, J.V. Woodside, “Antioxidants in health and disease”, J Clin Pathol, 54:176–86, 2001.
[23]. V. Lobo, A. Patil, A. Phatak, N. Chandra, “Free radicals, antioxidants and functional foods: Impact on human health”, Pharmacogn Rev, 4:118–26, 2010.
[24]. N. Riaz, S.A. Nawaz, N. Mukhtar, A. Malik, N. Afza, S. Ali, S. Ullah, P. Muhammad, M.I. Choudhary, Chemistry & Biodiversity, 4: 72, 2007.
[25]. E. Glotter, Natural Product Reports, 8: 415, 1991.
[26]. R.D. Budhiraga, S. Sudhir, Journal of Scientific Indian Research, 46: 488, 1987.
[27]. A.B. Ray, M. Gupta, Progress Chemical Organic Natural Products, 63: 1, 1994.
[28]. B. Halliwell, “How to characterize an antioxidant-an update”, Biochem Soc Symp, 61:73–101, 1995.
[29]. J.M. Crawford, C. Iacobuzio-Donahue, “Liver and biliary tract”, In: V. Kumar, A.K. Abbas, N. Fausto, J.C. Aster, Eds. Robbins and Cotran Pathologic Basis of Disease. 8th ed. Philadelphia,Pennsylvania, USA, Saunders, pp. 833–90, 2009.
[30]. D.L. Longo, A.S. Fauci, D.L. Kasper, S.L. Hauser, J.L. Jameson, J. Loscalzo, Harrison’s Principles of Internal Medicine. New York, USA, McGraw-Hill, Vol 2, 18th ed., 2011.
[31]. M. Nannipieri, C. Gonzales, S. Baldi, R. Posadas, K. Williams, S.M. Haffner, et al., “Liver enzymes, the metabolic syndrome, and incident diabetes: The Mexico City diabetes study”, Diabetes Care, 28:1757–62, 2005.
[32]. N.M. Concepcion, M.M. Pilar, A. Martin, J. Jimenez, U.M. Pilar, “Free radical scavenger and antihepatoxic activity of Rosmarinustomentous” Planta Medica, 59:312-314, 1993.
[33]. R.U. Ukpanukpong, J.O. Ajani, Wa. Omang, M.A. Adejorin, M.U. Eteng, E.U. Eyong, “Antidiabetic and antilipidemic effect of Khaya senegalensis Ethanolic bark extract in alloxan induced diabetic Wistar rats”, 7(8): 291-307, 2018.