Study on Estimation and Antioxidant activity of Gloriosa superba L. Whole Plant Extract

U. Jothi¹ , J. Angelin Jebamalar² , T. Sivakumar³

Section:Research Paper, Product Type: Isroset-Journal
Vol.6 , Issue.3 , pp.50-55, Jun-2019

CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v6i3.5055

Online published on Jun 30, 2019

Copyright © U. Jothi, J. Angelin Jebamalar, T. Sivakumar . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
Gloriosa superba is a alkaloid plant, which contains alkaloid components such as colchicine and gloriosine. In this study, Total Phenol content (TPC), Total Flavonoid (TF), Free radical scavenging activity (DPPH) using whole plant (Shoot, Flower, Tuber (SFT) extract derived from G. superba were tested for cancer. G. superba plants are identified and collected. The collected plants were separated into (SFT) G. superba whole plants are dried under the laboratory conditions. The temperature associated with the solvent used in the powder sampling will be followed by extraction using the different conical flask for 1 week and filtered what Mann No.1 paper. The filtrate was stored and used for further experimentation. Analysis of solvent extracts was subjected to phytochemical and antioxidative activity of DPPH assay. Different concentrations such as 20μg, 40μg, 60 μg, 80 μg and 100 μg were taken for all studies and the values are entered in terms of Mean±SD. In case of DPPH assay IC50 values are calculated using ANNOVA. Methanolic extraction from each solvent contains a high value of antioxidant properties.

Key-Words / Index Term :
Total Phenol Ccontent, Total Flavonoid, DPPH, G. superba

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