Potential Probiotic Analysis: Indigenous Lactic Acid Bacteria from Freshly Drawn Goat Milk

elvajeyanthi S¹ , Hemashenpagam N² , Vinotha M³

Section:Research Paper, Product Type: Isroset-Journal
Vol.6 , Issue.3 , pp.65-72, Jun-2019

CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v6i3.6572

Online published on Jun 30, 2019

Copyright © Selvajeyanthi S, Hemashenpagam N , Vinotha M . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
The raw goat milk is a good source of potential probiotic microorganisms. In developing countries, the goat milk was consumed widely because of its therapeutic and nutritional values. The potential probiotic bacteria has some properties such as, survival in the gastrointestinal conditions like low pH and bile tolerance, production of antimicrobial compounds and adhesion to intestinal mucosa. Thirty Lactic acid bacteria (LAB) isolated from goat milk in Tirupur and Erode region. Among 30 isolates, the nine isolates were selected for studying the characterization of potential probiotic of LAB. So the screening process were carried out like cell hydrophobicity, phenol resistance, antioxidant assay, cholesterol reducing activity, auto and co-aggregation, acid and bile tolerance. The results obtained showed that the isolates G13 has high spectrum of action. This isolate were undergo to detect the bacteriocin activity, column chromatography and SDS-PAGE. G13 isolates showed inhibitory activity in well-diffusion assay when tested against E.coli and S.aureus. So, those inhibitory substances are characterized as antimicrobial proteins called bacteriocins. Further identification of bacteriocin produced by G13 was confirmed by SDS-PAGE, where it shows the low molecular weight protein (17 kDa).

Key-Words / Index Term :
potential probiotic, cell hydrophobicity, phenol resistance, antioxidant assay, SDS-PAGE

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