Evaluation of Phytochemicals and In Vitro Anticancer Activity of Eupatorium Triplinerve

Suja G.¹ , Vinoth Kumar T.² , Suganya P.³

Section:Research Paper, Product Type: Journal-Paper
Vol.6 , Issue.5 , pp.15-18, Oct-2019

CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v6i5.1518

Online published on Oct 31, 2019

Copyright © Suja G., Vinoth Kumar T., Suganya P. . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

View this paper at   Google Scholar | DPI Digital Library

XML View     PDF Download

How to Cite this Paper

797 Views    495 Downloads    155 Downloads

Abstract :
The present study attempts to evaluate the phytochemical activity and in vitro anticancer activity of Eupatorium triplinerve methanol extract against lung cancer cell line A-549 and normal cell line Vero. The compound was analyzed for the presence of various phytoconstituents and screened for its cytotoxicity at five different concentrations by MTT assay. The phytochemical profile exhibited the presence of alkaloids, phenolics, flavonoids and steroids. The study documented that the percentage of cell viability has been reduced with the increased concentration, as evidenced by cell death. Eupatorium triplinerve extract shows potential cytotoxic activity with CTC50 of 450 µg/ml and 110 µg/ml against A-549 and Vero cell line respectively. The result obtained was comparably potent, the crude extract showed moderate activity against cancer cells and it was less toxic to the normal cells. In future, research need to be explored in the purification of this extract and identification of lead compound which could be used as a therapeutic tool against lung cancer

Key-Words / Index Term :
Anticancer, In vitro, Plant extract, Eupatorium triplinerve, Cytotoxicity, MTT assay

References :
[1]. S.K. Pal and B. Mittal B, “Fight against cancer in countries with limited resources: the post-genomic era scenario”, Asian Pacific Journal of Cancer Prevention, Vol. 5, Issue 3, pp. 328-33, 2004.
[2]. S. Alam, P. Satpathy, A. Thosar, “Plants and its parts as a source of Anti- Cancer Compounds: a Review”, International Research Journal of Pharmacy. Vol.5, Issue 4, pp. 244-249, 2014.
[3]. W. Kooti, K. Servatyari, M. Behzadifar, “Effective Medicinal Plant in Cancer Treatment, Part 2: Review Study”, Journal of Evidence-Based Complement Alternative Medicine, Vol. 22, Issue 4, pp.982-995, 2017.
[4]. E. Mansouri, W. Kooti, M. Bazvand, “The effect of hydro-alcoholic extract of Foeniculum vulgare Mill on leukocytes and hematological tests in male rats”. Jundishapur Journal of Natural Pharmaceutical Products. Vol.10, pp. e18396, 2015.
[5]. W. Kooti, M. Ghasemiboroon, M. Asadi-Samani, “The effects of hydro-alcoholic extract of celery on lipid profile of rats fed a high fat diet”, Advances in Environmental Biology. Vol.8, pp.325–330, 2014.
[6]. W. Kooti, Z. Hasanzadeh-Noohi, N. Sharafi-Ahvazi, M. Asadi-Samani, D. Ashtary-Larky, “Phytochemistry, pharmacology and therapeutic uses of black seed - Nigella sativa”, Chinese Journal of Natural Medicine. Vol.14, pp.732–745, 2016.
[7]. Anees Ahmed Siddique and Mohammed Ali. Practical Pharmaceutical Chemistry, CSB publishiers, India, pp.126-132, 2008.
[8]. W. Strober, ‘Tryphan blue exclusion test of cell viability”, Current Protocol of Immunology, John Wiley & Sons publishers, U.K, pp. A3.B.1- A3.B.2D, 1997
[9]. A. Scudiero, R.H. Shoemaker, K. D. Paul, “Evaluation of soluble Tetrazolium Formazan assay for cell growth and drug sensitivity in cultures using human and other tumor cell lines”, Cancer Research. Vol. 48, pp. 4827- 4833, 1998.
[10]. P.G. Pietta, “Flavonoids as antioxidants”, Journal of Natural Products. Vol.63, pp. 1035-1042, 2000.
[11]. S. Yogisha and K. A. Raveesha, “In-vitro antibacterial effect of selected medicinal plant extracts”, Journal of Natural Products, Vol. 2, pp.64-69, 2009.
[12]. M.S. Uma Maheswari, R. Rajendran, “Comparative Evaluation of Qualitative and Quantitative Phytochemical Analysis of South Indian Bioactive Medicinal Plant Tribulus terrestris”, International Journal of Scientific Research in Biological Sciences, Vol.5, Issue.6, pp.144-149, 2018.
[13]. K. F. Sigaroodi, M. Jeddi-Tehrani, M. Ahvazi M, “Cytotoxicity evaluation of Taverniera spartea on human cancer cell lines”, Journal of Medicinal Plants. Vol.2. pp.114-128, 2014.
[14]. J. Guna Priya, K. Geetha Lakshmi, Niharika Sharma, D. Rajani, “A Comparative Study of Antioxidants and Antimicrobial activity in Vegetables, Leafy Vegetables and Spices”, International Journal of Scientific Research in Biological Sciences, Vol.06, Issue.01, pp.80-85, 2019.