Long-Term Exposure of Arsenic Caused Arsenic Bioaccumulation, Imbalance in Hepatic Markers, Oxidative Status, and Histological Changes in the Liver of Zebrafish (Danio Rerio)

K. Bhavani¹ , R. Karuppasamy²

Section:Research Paper, Product Type: Journal-Paper
Vol.6 , Issue.6 , pp.32-38, Dec-2019

CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v6i6.3238

Online published on Dec 31, 2019

Copyright © K. Bhavani, R. Karuppasamy . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
The present experiment was conducted to analyze the effect of sub-lethal concentration of 1.11mg/l of arsenic (III) oxide over long-term (15, 30, 45, 60 days) exposure on the liver of zebrafish. The arsenic (III) oxide exposed zebrafish liver showed a significant increase in arsenic bioaccumulation with the increase of exposure duration. The activities of lipid peroxidation (LPO) markers like thiobarbituric acid reactive substances (TBARS) and hydrogen peroxide (H2O2) were also significantly increased with the increase of arsenic exposure duration, while the antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), superoxide dismutase (SOD) and reduced glutathione (GSH) activities were notably increased during the initial short term arsenic exposure (15 and/or 30 days), but later decreased significantly in the fishes that were exposed to arsenic for an extended period (45 & 60 days) when compared to normal. Further, the hepatic enzymes like alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP) levels were significantly increased in serum, and degenerative changes were also found in the histology of the zebrafish liver. Thus, the above results suggest that long-term exposure to arsenic can lead to bioaccumulation of arsenic as well as alter the function and tissue architecture of the zebrafish liver.

Key-Words / Index Term :
Arsenic; long-term exposure; lipid peroxidation; zebrafish; liver

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