Cytotoxic Activity of Phenolic Compound Isolated From Pandanus Odorattissimus against (MDA-MB-231) Breast Cancer Cell Lines

Abhaykumar Kamble¹ , Paramjyothi L Swamy²

Section:Research Paper, Product Type: Journal-Paper
Vol.6 , Issue.6 , pp.39-46, Dec-2019

CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v6i6.3946

Online published on Dec 31, 2019

Copyright © Abhaykumar Kamble, Paramjyothi L Swamy . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
The present study reports potential activities like cytotoxic, genotoxic and apoptotic activity of phenolic compound 4-(4-(3,4-dimethoxyphenyl)hexahydrofuro[3,4-c]furan-1-yl)-2-methoxyphenyl acetate isolated from methanolic extract of Pandanus odorattissimus. The compound showed a significant cytotoxic effect on MDA-MB-231 cell line. Exposure of the compound reduced the viability of MDA-MB-231 cells after 24, 48 and 72 hours, the compound exerted a significant cytotoxic effect on MDA-MB-231 cells. The compound also induced significant DNA damage. The results of comet assay with pattern of the MDA-MB-231 cells has shown an intact head and complete absence of DNA fragments in the form of tail suggesting that the doses are not genototoxic. The phenolic compound at concentration of 20 µg/mL, showed an increase in percentage tail of DNA upto 5.87 units when compared to control 5.05.

Key-Words / Index Term :
Cytotoxic activity, Comet assay, Pandanus odorattissimus L, MDA-MB-231 cells.

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