In Vitro Propagation of Endangered Medicinal Plant Ormocarpum Sennoides (Willd.) DC From Internodal Segments

G. Nandhini¹ , J. Anbumalarmathi² , K. Mercy Madhumitha³

Section:Research Paper, Product Type: Journal-Paper
Vol.7 , Issue.1 , pp.84-89, Feb-2020

Online published on Feb 28, 2020

Copyright © G. Nandhini, J. Anbumalarmathi, K. Mercy Madhumitha . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
An efficient regeneration protocol was established for in vitro propagation through direct and indirect regeneration of Ormocarpum sennoides from internodal explants. Murashige and Skoog (MS) medium supplemented with different concentrations of 6-benzyladenine amino purine (BAP), 2, 4 Dichlorophenoxy acetic acid (2,4-D), Indole-3-acetic acid (IAA) and Napthalene acetic acid (NAA) either alone or in combinations. Indirect regeneration, MS medium supplemented with D1: BAP 2.5 mg/L has shown the highest regeneration percentage of 88.9% with shoot length of 8.36 cm and root length of 1.52 cm. In indirect regeneration I4: BAP 2.0 mg/L + IAA 3.0 mg/L has shown the highest callus induction percentage of 86.7% and maximum callus weight of 1.03g. The highest frequency of shoot regeneration (68.3%) with maximum shoot length (3.53 cm) was observed in MS medium supplemented with IS5: BAP 3.5 mg/L. The highest frequency of root regeneration (56.1%) with maximum root length (1.53 cm) was observed in MS medium supplemented with IR5: IAA 2.5 mg/L + NAA 1.0 mg/L. The in vitro derived rooted plantlets were successfully hardened and were acclimatized in the greenhouse.

Key-Words / Index Term :
Ormocarpum sennoides, endangered medicinal plant, direct and indirect regeneration, hardening

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