Rediscovering the Diversity of Guatemalan Bumble Bees (Hymenoptera: Apoidea: Bombus Latreille, 1802)

Dardón MJ¹ , Yurrita CL² , Landaverde-González P³ , Vásquez M⁴ , Ayala R.⁵

Section:Research Paper, Product Type: Journal-Paper
Vol.7 , Issue.2 , pp.1-14, Apr-2020

Online published on Apr 30, 2020

Copyright © Dardón MJ, Yurrita CL, Landaverde-González P, Vásquez M, Ayala R. . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
Bumble bees (Bombus Latreille) play an essential role as pollinators of both wild and cultivated plants. Traditionally, these bees have been identified based on their color patterns, which in some cases vary among individuals of the same species, or different species, can share similar patterns and colorations allowing for inaccurate identifications. Despite these problems, Guatemalan reports on Bombus species have relied basically on color patterns. Here, we combine mitochondrial DNA barcode sequences and morphometric measurements, in an attempt to provide further information on the species limits of nine Bombus species occurring in Guatemala and also two species occurring in Mexico. When molecular analyses failed to distinguish between “morphological species” we applied traditional morphometrics using body measurements. Analyses of mitochondrial DNA sequences allowed for the discrimination of seven of the eleven “morphological species” evaluated. The remaining four species clustered into two pairs of species that have problematic taxonomy (B. sonorous–B. pensylvanicus, and B. ephippiatus–B. wilmattae). Morphometric analysis performed on the B. ephippiatus–B. wilmattae pair indicated non-discrimination between these forms, supporting the hypothesis that these are a single species. Therefore, based on our results we confirm the presence of the following species in Guatemala: B. ephippiatus (including B. wilmattae as synonym), Bombus (Cullumanobombus) macgregori Labougle & Ayala, 1985, Bombus (Thoracobombus) medius Cresson, 1863, Bombus (Thoracobombus) mexicanus Cresson, 1878, Bombus (Thoracobombus) pullatus Franklin, 1913, Bombus (Psithyrus) variabilis (Cresson, 1872), Bombus (Thoracobombus) weisi Friese, 1903, and Bombus (Thoracobombus) xelajuensis Asperen de Boer, 1992.

Key-Words / Index Term :
DNA barcoding, Bombini, Central America, Morphometry, Taxonomy

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