GC-MS Analysis of Bioactive Compounds in Methanolic Extract of Marine Enterobacter Cloacae Mariam ADRA1*, Badr ALALI2 and Ahmad KARA ALI3

Mariam ADRA¹ , Badr ALALI² , Ahmad KARA ALI³

Section:Research Paper, Product Type: Journal-Paper
Vol.7 , Issue.2 , pp.62-68, Apr-2020

Online published on Apr 30, 2020

Copyright © Mariam ADRA, Badr ALALI , Ahmad KARA ALI . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
Antimicrobial substances are so widespread that they are likely to play an important therapeutic role. Many researches showed that microorganisms produce important biochemical compounds, which were used in different domains (medical, pharmaceutical, industrial). The present study aimed to isolate a marine bacterium and study the bioactivity of its methanolic crude extracts. Marine water samples were collected for the isolation of bacteria based on their growth density and then identified depended on morphological, microscopic characterization and biochemical tests. The antibiotics sensitivity was estimated by DL-96II Auto Microbial ID/AST System, While the effectiveness of the methanolic extract was determined by agar- well diffusion method. The compounds in the organic extract of the isolated bacteria were identified by Gas chromatography – mass spectrometry (GC-MS), and their bioactivity was studied. The results showed that these bacteria is Enterobacter cloacae and showed resistance to six from twenty antibiotics. The bioactivity of extract was higher against Pseudomonas aeruginosa and Escherichia coli than Staphylococcus aureus. The chemical analysis of the organic extract showed that eighteen chemical compounds have medical and pharmaceutical importance.

Key-Words / Index Term :
Marine Enterobacter cloacae, Antibiotics, Bioactivity, GC-MS analysis

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AUTHORS PROFILE
Mrs. Mariam Adra has obtained B. of Microbiology from Tishreen University in 2016, and is studying MSc of Marine Biology science at the Higher Institute for Marine Research. She works as a science teacher at the secondary level and teaches bacteriology and antibiotics at the university. Her main research work focuses on bacteria. she has 5 years of teaching experience and 3 years of research experience.