Bacteriocin Induced Milk as a Vehicle against Dental Cavities

Parul Thapar¹ , R.K. Malik² , M.K. Salooja³

Section:Research Paper, Product Type: Journal-Paper
Vol.7 , Issue.2 , pp.69-76, Apr-2020

Online published on Apr 30, 2020

Copyright © Parul Thapar, R.K. Malik, M.K. Salooja . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
The prevalence of dental caries is consistently increasing, in every age across the globe, and today the concern for the “Oral health” is almost at the same level as for “General health”. The oral health care demands are beyond the capacities of the health care systems in most low and middle income countries. Therefore, it was thought to explore the natural ways by which the problems of dental caries can be prevented in a cost-effective manner. It was found that a few studies have set the path through use of natural bacteriocins against inhibition of caries causing organisms. The inhibition of caries causing organisms can be done by naturally occurring substances like bacteriocins found in lactic acid bacteria. Bacteriocins are potent protein toxins produced by virtually every bacterial and archeal species. In the present study, prevention of dental caries by utilization of bacteriocins from lactic acid bacteria has been done by incorporating bacteriocin in milk. The bacteriocin producing five strains of Lactobacillus brevis isolated from curd were used for the production of bacteriocin. The minimum inhibitory concentration (MIC) of the bacteriocins was carried out to find the best bacteriocin producing strain. Out of five bacteriocin producers, the strain LB11(1) showed maximum percentage of MIC. The characterization of the bacteriocin was done using various optimization conditions. The partial purification was done using ammonium sulphate precipitation and centrifugal filteration. The purified bacteriocin was then incorporated in the milk to check the effect of inhibition on caries causing organisms (Streptococcus mutans and Streptococcus sobrinus isolated from dental cavities). It was confirmed that the bacteriocin induced milk can be applied to prevent dental caries directly by the consumer and the milk itself can become a vehicle against dental cavities.

Key-Words / Index Term :
Bacteriocins, lactic acid bacteria, Streptococcus mutans, Streptococcus sobrinus, Lactobacillus brevis

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