Phytochemical Screening and Analyses of Eight Selected Heavy Metals in an Air-dried and Sun-dried Leaf of Alternanthera brasiliana (L.) O Kuntze

Arogbodo J.O¹ , Ajayi ² , O.A ³ , Adebayo I.A⁴

Section:Research Paper, Product Type: Journal-Paper
Vol.7 , Issue.2 , pp.146-150, Apr-2020

Online published on Apr 30, 2020

Copyright © Arogbodo J.O, Ajayi, O.A, Adebayo I.A . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
The growing interest in ethno-medicine calls for effective laboratory scrutiny of the part(s) of the medicinal plant(s) being used as raw materials in the preparation of useful products as a way of certifying their bio-safety. To achieve this aim, air-dried and sun-dried leaves of Alternanthera brasiliana (L.) were subjected to preliminary analyses of its phytochemicals and some heavy metals using standard methods. The results confirmed the presence of saponins, tannins, flavonoids, steroids, terpenoids, cardiac glycosides, and absence of phlobatannins, alkaloids, and anthraquinones in both in air-dried (ADS) and sun-dried samples (SDS). The quantity of the phytochemicals were found to be in decreasing order of saponins > terpenoids > steroids > cardic glycosides > flavonoids > Tannins and the heavy metal values also in the order of Fe > Zn > Cu > Mn > Se > Cd > Co > Pb. The heavy metals in the two samples were within the permissible safe limits recommended by Food and Agriculture Organization (FAO) and World Health Organization (WHO); indicating that the leaf of A. brasiliana is not toxic of the analyzed metals. Likewise, it was deduced from the results that air-drying could be a preferred processing method in the preparation of A. brasiliana leaf as more of the phytochemicals and metals were found in the air-dried than the sun-dried leaf sample.

Key-Words / Index Term :
Heavy metal, phytochemical screening, processing methods, A. brasiliana

References :
[1] S. Yogesh, G. Jeyabalan, S. Ramandeep, “Potential Wound Healing Agents from Medicinal Plants: A Review.” DOI: 10.5567 Pharmacologia 4 (5): 349 – 358, 2013.
[2] E. A. Oluyemi, G. Feuyit, J. A. O. Oyekunle, A. O. Ogunfowokan, “Seasonal variations in heavy metal concentrations in soil and some selected crops at a landfill in Nigeria”. African Journal of Environmental Science and Technology 2 (5): pp. 089-096, 2008.
[3] WHO, “World Health Organization. Arsenic.” Available at: https://www.who.int/news-room/fact-sheets/detail/arsenic, 2018.
[4] K. Bhavani, R. Karuppasamy, “Long-Term Exposure of Arsenic Caused Arsenic Bioaccumulation, Imbalance in Hepatic Markers, Oxidative Status, and Histological Changes in the Liver of Zebrafish (Danio Rerio).” International Journal of Scientific Research in Biological Sciences. 6(6): 32 – 38, 2019.
[5] M. Jaishankar, T. Tseten, N. Anbalagan, B. B. Matthew, K. N. Beeregowda, “Toxicity, mechanism and health effects of some heavy metals”. Interdisciplinary Toxicology. 7(2): 60 – 72, 2014.
[6] K. Saawan, S. Pradeep, M. Garima, S. Saurabh, K. K. Jha, L. Khosa, “Phytopharmacological review of Alternanthera brasiliana (Amaranthaceae)”. Asian Journal of Plant Science and Research. 1(1): 41 – 47, 2011.
[7] M. R. Duarte, M. C. Debur, Brazilian Journal of Pharmaceutical Sciences, 40 (1); 85 – 92, 2004.
[8] R. W. Bussann, A Glenn, D. Sharon, Indian Journal of Traditional Knowledge, 9(4): 742 – 753, 2010.
[9] C. C. Barua, A. Talukdar, S. A. Begum, D. K. Sarma, D. C. Fathak, A. G. Barua, R. S. Bora, “Wound healing activity of methanolic extract of leaves of Alternanthera brasiliana Kuntz using in vivo and in vitro model” Indian J. Exp. Bio., 47: 1001–1005, 2009.
[10] A. F. Macedo, C. L. Lage, M. A. Esquibel, M. M, Souza, K. L. Silva, “Preliminary phytochemical and pharmacological studies on plantlets of Alternanthera brasiliana cultured under different spectral quality of lights.” Acta Farm bonaerense 23 (4): 515 – 519, 2004.
[11] J. B. Harbone, “Phytochemical methods: guide to modern techniques of plant analysis. Chapman and hall Ltd, London”, pp 49 – 50, 1973.
[12] K. R. Brain, T. D. Turner, “Practical evaluation of phytopharmaceuticals”. Wright-Scientechnia. 1st Ed. Bristol, 1975.
[13] J. H. Brunner, “Direct spectrophometer dertermination of saponin”. Animal Chemistry 34: 1314- 1326, 1984.
[14] A. Sofowora, “Medicinal plants and traditional medicine in Africa. Spectrum Books Ltd., Ibadan, Nigeria, Africa”, pp 191 – 290, 1993.
[15] C. O. Brochado, A. P. Almeida, B. P. Barreto, L. P. Costa, L. S. Ribeiro, R. L. C. Pereira, V. L. G. Koatz, S. S. Costa, Journal of Braz. Chem. Soc., 14 (3): 449 – 451, 2003.
[16] L. Järup, “Hazards of heavy metal contamination”, British Medical Bulletin, 68: 167 – 182, 2003.
[17] WHO, “Evaluation of Certain Food Additives and Contaminants (Thirty-third report of the Joint FAO/WHO Expert Committee on Food Additives)”. WHO Technical Report Series, No. 776, 1989.
[18] WHO, “Quality Control Methods for Medicinal Plant Materials”. World Health Organization, Geneva, 1998.
[19] U. Divrikli, N. Horzum, M. Soylak, L. Elci, “Trace Heavy Metals Contents of some Spices and Herbal Plants from Western Anatolia, Turkey”, International Journal of Food Science Technology, 41: 712 – 716, 2006.
[20] FAO, “Water quality for irrigation for agriculture”, Irrigation Drainage paper, FAO (MGKG-1) Recommended maximum concentration of trace elements for crop production 29: 1- 130, 1985.
[21] WHO, “Quality Control Methods for Medicinal Plant Materials”. World Health Organization, Geneva, Switzerland, 2005.
[22] M. N. L. Maffo, L. Zapfack, E. Youmbi, S. D. Dibong, G . Ntsomboh-Ntsefong, A. D. Nanfack, “Heavy Metals Concentrations in some Common Medicinal Plants from Different Geographical Locations in Douala, Cameroon”. International Journal of Current Research in Biosciences and Plant Biology. 3(12): 10 – 23, 2016.
[23] F. J. Ager, J. R. Ynsa, J. R. Domínguez-solís, L. C. Romero, “Cadmium localization and quantification in plant Arabidopsis thaliana using micro-PIXE. Nucl. Instrum. Meth”. Phys. Res. 189: 494 – 498, 2002.