Isolation and Identification of Fungi from Peanut Samples Sold at Adama City Markets, Oromia Regional State, Ethiopia

Tseday Temesgen¹ , Bayissa Chala²

Section:Research Paper, Product Type: Journal-Paper
Vol.7 , Issue.3 , pp.79-84, Jun-2020

Online published on Jun 30, 2020

Copyright © Tseday Temesgen, Bayissa Chala . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
Peanut is a legume crop that belongs to the family of Fabaceae, genus Arachis, and botanically named as Arachis hypogaea. Peanuts have been consumed in a variety ways in Adama city perhaps with poor hygiene condition. The major consumers of peanuts in Adama are Khat chewers and people who may consume roasted barely grain with nuts. Fungal contamination associated with peanut seeds sold at Adama city markets and streets were examined in the present study. Examination was performed depending on the morphological features of the fungal genera. Potato dextrose agar (PDA) was prepared and autoclaved at 121oC for 15min. Peanut seeds were surface sterilized with 70% alcohol and rinsed with water and plated in the petri dish containing PDA. Incubation temperature was 25oC and the fungal growth were observed for 5 days. The isolated fungi were then sub cultured on another prepared PDA and used for identification. From the observed growth features and color of the fungal colonies, four taxa of fungi were examined and those were Aspergillus species, Penicillium species, Fusarium species and Mucor species. The study revealed that almost all the 225 peanut samples were infected by one of the four fungal genera. Aspergillus species were identified to be the most prevalent isolates with a percentage of 45.5% followed by Penicillium (32.9%). The rest Fusarium species and Mucor species were the least prevalent with proportion of 10.9% and 10.5%, respectively. The hot and arid climate of the city appears to be one main reason for the acceleration of the fungal growth in peanut. Further surveillance and setting regulation by the government on seed pathogens will play a great role in reduction of those fungi.

Key-Words / Index Term :
aflatoxin, fungi, groundnut, peanut

References :
[1] E. Guchi, ?Aflatoxin Contamination in Groundnut (Arachis hypogaea L.) caused by Aspergillus species in Ethiopia,? Journal of Applied & Environmental Microbiology, Vol. 3, Issue.1, pp.11-19, 2015.
[2] USDA, National Nutrient Database for Standard Reference, 2011.
[3] A. Amare, A. Dawit, H. Mengistu, ?Micoflora, aflatoxin resistance of groundnut cultivars from eastern Ethiopia,? SNET, Ethiopian Journal of Science, Vol. 18, pp.17- 130, 1995.
[4] Central Statistical Authority (CSA) of the Federal Democratic Republic of Ethiopia, Central Agricultural Census Commission, ?Ethiopian agricultural sample enumeration?, Statistical Report on area and production of Crops, Volume I, Addis Ababa, 2014.
[5] L.A. Agyei, Aflatoxin -A silent killer. http://www.ghanabusinessnewsCom/2013/04/16/ aflatoxin-a-silent-killer/. Accessed: March, 2020, 2013.
[6] FAO-World Bank, ?Reducing post-harvest losses in grain supply chains in Africa?. Report of FAO-World Bank workshop held from 18?19th March, 2010 in Rome, Italy. pp. 120, 2010.
[7] J.L. Davison, T.B. Whitaker, J.W Dickens, ?Grading, cleaning, storage, shelling and marketing of peanut in the united state,? In: Patte, H.E., Young, C.T. (Eds.), Peanut Science and Technology. Yoakum, American Peanut Research and Education Society, pp. 571-623, 1982.
[8] A. Chala, B. Abate, M. Taye, A. Mohammed, T. Alemu, H. Skinnes, ?Opportunities and constraints of groundnut production in selected dry lands of Ethiopia,? Dry lands Coordination Group, Milj?huset G9, Norway, 2014.
[9] R.J. Cole, J.W. Dorner, C.C. Holbrook, ?Advances in mycotoxin elimination and resistance?. Advances in Peanut Science. American Peanut Research and Education Society, Stillwater, OK, pp.456-474, 1995.
[10] P.E. Sumner, D. Lee, ?Reducing Aflatoxin in corn during harvest and storage. Atlanta, GA?. The University of Georgia, Georgia College of Agriculture and Environmental Sciences. 2012
[11] T. Marta, K. Bedaso, ?Occurrence, importance and control of mycotoxins: A review,? Cogent Food and Agriculture, Vol. 2, Issue.1, pp.1191103, 2016.
[12] J.M. Milani, ?Ecological conditions affecting mycotoxin production in cereals: a review?. Veterinarni Medicina, Vol. 58, Issue.8, pp.405-411, 2013.
[13] Central Statistical Agency (CSA). Statistical Abstract of Ethiopia. Addis Ababa, Ethiopia: Central Statistical Agency, 2009.
[14] I. Abuga, ?Isolation and identification of fungi associated with groundnut seed sold at aliero central market,? International Journal of Biological Science, Vol. 1, Issue.5, pp.56-62, 2014.
[15] M.A. Khiyami, M.A. El-Naggar, H. Almoammar, K.A. Abd-Elsalam, ?Mycological, biochemical and molecular methods for detection of aflatoxigenic Aspergilli from peanut kernels,? The Philippine Agricultural Scientist, Vol. 98, Issue.1, pp.52-59, 2015.
[16] D.O. Okun, F.M. Khamis, G.M. Muluvi, J.J. Ngeranwa, F.O. Ombura, M.O. Yongo, E.U. Kenya, ?Distribution of indigenous strains of atoxigenic and toxigenic Aspergillus flavus and Aspergillus parasiticus in maize and peanuts agro-ecological zones of Kenya,? Agriculture and Food, Vol. 4, pp.14, 2015.
[17] H. Nyirahakizimana, L. Mwamburi, J. Wakhisi, C.K. Mutegi, M.E. Christie, J.M. Wagacha, ?Occurrence of Aspergillus species and Aflatoxin Contamination in Raw and Roasted Peanuts from Formal and Informal Markets in Eldoret and Kericho Towns, Kenya,? Advances in Microbiology, Vol. 3, Issue.4, pp.333-342, 2013.
[18] C. M. Abraham, T. M. ?Thomas Emerging fungal contaminants isolated and identified from raw fennel seeds,? International Journal of Scientific Research in Biological Sciences Vol.5, Issue.3, pp. 32-35, 2018.
[19] E.M. Embaby, M.M. Abdel-Galel, ?Detection of fungi and aflatoxins contaminated peanut samples (Arachis hypogaea L.),? Journal of Agricultural Technology, Vol. 10, Issue.2, pp.423-437, 2014.
[20] J.A. Cray, Bell, A.N. Bhaganna, P. A.Y. Mswaka, D.J. Timson, J.E. Hallsworth, ?The biology of habitat dominance; can microbes behave as weeds?? Microbial Biotechnology, Vol. 6, pp.453?492, 2013.
[21] S. Amaike, N.P. Keller, Aspergillus flavus. ?Annual Review of Phytopathology,? Vol. 49, pp.107?133, 2011.
[22] V. Kumar, M.S. Basu, T.P. Rajendran,. ?Mycotoxin research and mycoflora in some commercially important agricultural commodities,? Crop Protection, Vol. 27, pp.891?905, 2008.