A Study on Phytochemical Screening and Analysis of Leaf Extract of Withania Somnifera

S. Fathaunnisha¹ , V. Hemamalini² , H. Jayasudha³ , R. Sridhar⁴

Section:Research Paper, Product Type: Journal-Paper
Vol.7 , Issue.4 , pp.30-34, Aug-2020

Online published on Aug 31, 2020

Copyright © S. Fathaunnisha, V. Hemamalini, H. Jayasudha, R. Sridhar . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

View this paper at   Google Scholar | DPI Digital Library

XML View     PDF Download

How to Cite this Paper

Abstract :
Withania somnifera is a highly renowned plant in the Indian Ayurvedic and Unani System of medicine. This plant is known to promote physical and mental health and is used to treat almost all of the disorders that affect human health. So, it is called as? Power Herb?. The present study investigated the qualitative and quantitative phytochemical analysis of W. somnifera. The leaves were shade dried and powdered and sequentially extracted with Hexane, Ethyl acetate, and Ethanol solvents. Qualitative and Quantitative Phytochemical analysis was performed for the three extracts. The phytochemical analysis showed the presence of carbohydrates, tannins, saponins, flavonoids, alkaloids, quinones, phenols, etc., Quantitative estimation of the phytochemicals was also evaluated. Biologically active phytochemicals were mainly present in the Ethyl acetate extract of W. somnifera

Key-Words / Index Term :
Amukkura, Extraction, Phytochemical analysis, Tannins, Flavonoids

References :
[1] Rege, N N et a., ?Adaptogenic properties of six rasayana herbs used in Ayurvedic medicine.? Phytotherapy research: PTR Vol. 13, Issue 4, pp. 275-91, 1999.
[2] Pandey. S.S, Singh, S., Pandey, H. et al, ?Endophytes of Withania somnifera modulate in planta content and the site of withanolide biosynthesis.? Scientific reports Issue 8, pp. 5450, 2018.
[3] Gupta, Parul et al, ?Comparative transcriptome analysis of different chemotypes elucidates withanolide biosynthesis pathway from medicinal plant Withania somnifera.? Scientific reports Vol. 5 18611, 21 Dec. 2015 doi: 10.1038/srep18611.
[4] Sukanya M.S., Archana Giri, "Enhanced Production of Therapeutically Valuable Withaferin A from In Vitro Propagated Withania Somnifera (L.) Dunal," International Journal of Scientific Research in Biological Sciences?, Vol.7, Issue.1, pp.1-6, 2020
[5] Trease GE, Evans WC, ? Pharmacognosy?, 12th edition, Bailliere Tindal, Eastbourne, pp. 136-204, 1983.
[6] Harborne JB, ?Phytochemical methods; A Guide to Modern Techniques of plants Analysis?, 1st Edition, Chapman and Hall, Madras, pp.302, 1998.
[7] Sofowora A, ?Medicinal Plants and Traditional Medicinal in Africa?. 2nd Edition, Sunshine House, Ibadan, Nigeria: Spectrum Books Ltd. Screening Plants for Bioactive Agents, pp. 134?56, 1993.
[8] Sakanaka S, Tachibana Y., Okada Y. ?Preparation and antioxidant properties of extracts of Japanese persimmon leaf tea (kakinohacha)? Food Chemistry, Vol. 89, Issue 4, pp. 569-575, 2005.
[9] Amin Mir M, Sawhney SS, Jassal MMS, ?Qualitative and quantitative analysis of phytochemicals of Taraxacum officinale?. Wudpecker Journal of Pharmacy and Pharmocology, Vol. 2, Issue 1, pp. 1-5, 2013.
[10] Linuma M, Tsuchiya H, Salo M, Yokoyama J, Ohyama M, Ohkawa Y, ?Flavanones with potent antibacterial activity against methicillin-resistant Staphylococcus aureus, J Pharm Pharmacol, Vol. 46, Issue 11, pp. 892-895, 1994.
[11] Tholl D, ?Biosynthesis and biological functions of terpenoids in plants, Adv Biochem Eng Biotechnology , Vol. 148, pp. 63-106, 2015.
[12] Killedar SG, More HN, ?Estimation of tannins in different parts of Memecylon umbellatum Burm?. J Phar Res, Vol. 3(3), pp. 554-556, 2010.
[13] Abduljalil, Jameel & Al-Rakhami, Asmaa & Al-Haj, Thabet & Al-Rrimy, Ali & Al-Wheabi, Amatallah, ?Preliminary Phytochemical Analysis and Antibacterial Activity of Methanol Extracts from Origanum majorana, Rumex nervosus, and Withania somnifera.? Int J Pharma Res Health Sci, Vol. 6, Issue 6, pp-2844-2850, 2018.
[14] Bashir, H. S., Mohammed, A. M., Magsoud, A. S., & Shaoub, A. M, "Isolation of three flavonoids from Withania somnifera leaves (Solanaceae) and their antimicrobial activities." Journal of Forest Products and Industries Vol. 2, Issue 5, pp. 39, 2013.