Prevalence and Susceptibility Profiles Of Staphylococcus Aureus Isolates from Outpatients and Inpatients at UCTH, Calabar, Nigeria

Otu ¹ , J.U. ²

Section:Research Paper, Product Type: Journal-Paper
Vol.7 , Issue.5 , pp.140-146, Oct-2020

Online published on Oct 31, 2020

Copyright © Otu, J.U. . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
This study determined the prevalence and susceptibility profiles of Staphylococcus aureus isolates from patients at University of Calabar Teaching Hospital (UCTH), Calabar, Cross River State, Nigeria. 120 swab specimens from patients were screened for staphylococci using standard microbiological protocol. Coagulase-positive staphylococci (CoPS) isolates were subjected to 16S rRNA sequencing for further identification. By disk diffusion method, antibiotic susceptibility test was evaluated in all the S. aureus strains. Results revealed that 46 (38.3%) CoPS and 52 (43.1%) coagulase-negative staphylococci (CoNS) were isolated. Morphological and biochemical characterization showed that of the 120 samples used, 98 (81.7%) yielded staphylococci, out of which only 9 strains of S. aureus were identified on the basis of 16S rRNA sequencing. The gender distribution ratio of prevalence of staphylococci was 25:21 (54.3%:45.7%) female to male but with male showing preponderance. There was no significant relationship between sex of patients and prevalence rate of CoPS at significant level (P?0.05). All the S. aureus strains were highly susceptible to ceftriaxone and cefixime and resistant to vancomycin, penicillin and chloramphenicol. There was significant difference (p?0.05) in the resistance patterns of the 9 strains of S. aureus. The results proved that S. aureus isolates from the clinical samples are relatively resistant to commonly used antibiotics. The outcome of this study is useful in moderating the right prescription of antibiotics against S. aureus infections especially in hospital settings.

Key-Words / Index Term :
Susceptibility, prevalence, resistance

References :
[1]. G. Dayan, N. Mohammed, I. Scully, D. Cooper, E. Begier. “Staphylococcus aureus: the current state of disease, pathophysiology and strategies for prevention”. Expert Reviews in Vaccines, Vol. 9, No. 2, pp.1-20, 2016.
[2]. F. Devapriya, R.S. Ramesh, A.K. Khan, J. Shanmugam. “?-lactamase production of Staphylococcus aureus: a comparison study of different iodometric methods”. Gulf Medical Journal, Vol. 2, No. 1, pp.16-21, 2013.
[3]. L.S. Mohammed, M.T. Flayyih. “Isolation and Identification of Methicillin Resistance Staphylococcus aureus from Clinical Samples and their Drug Resistance Patterns”. World Journal of Experimental Biosciences, Vol. 4, No. 2, pp.171-175, 2016.
[4]. A.A. Akindele, I.K. Adewuyi, O.A. Adefioye, S.A. Adedokun, A.O. Olaolu. “Antibiogram and beta-lactamase production of Staphylococcus aureus isolates from different human clinical specimens in a tertiary hospital institution in Ile-Ife, Nigeria”. American Journal of Scientific Research, Vol. 5, Issue. 6, pp.230-3, 2010.
[5]. A.R. McCullough, A.J. Pollack, M.P. Hansen, P.P. Glasziou, D.F. Looke, H.C. Britt. “Antibiotics for acute respiratory infections in general practice: comparison of prescribing rates with guideline recommendations”. Medical Journal of Australia, Vol. 207, No. 4, pp.65-69, 2017.
[6]. S.T. Chancey, D. Zähner, D.S. “Acquired inducible antimicrobial resistance in gram-positive bacteria”. Future Microbiology, Vol. 7, Issue. 4, pp.959-978, 2012.
[7]. N. Poddar, D. Pattnaik, K. Panigrahi, B. Pathi, P.R. Lenka, S. Mohanty, B. Mallick, J. Jena. “Prevalence and Antimicrobial Susceptibility Pattern of Methicillin-resistant Staphylococcus aureus (MRSA) Isolates and Inducible Clindamycin resistance in Staphylococcus aureus: study at a Tertiary Care Hospital in KIMS, Bhubaneswar, India”. International Journal of Scientific Research in Biological Sciences, Vol. 2, Issue. 1, pp.9–15, 2015.
[8]. J.M. Munita, C.A. Arias. “Mechanisms of antibiotic resistance”. Microbiology Spectrum, Vol. 4, pp.10-14, 2016.
[9]. F.R. DeLeo H.F. Chambers. “Re-emergence of antibiotic-resistant Staphylococcus aureus in the genomics era”. Journal of Clinical Investigation, Vol. 119, Issue. 10, pp.2464-2474, 2009.
[10]. M. Nagel, T. Revter, A. Jansen. “Influence of ciprofloxacin and vancomycin on mutation rate and transposition in Staphylococcus aureus”. International Journal of Medicine and Microbiology, Vol. 301, No. 8, pp.229-236, 2011.
[11]. A. Pugazhendhi, M. Dexilin, P. Deepika, P.K. Poornima, S. Rajasree, A.A. Naif, D. Veeramuthu, V.A. Mariadhas, K. Thamariselvi. “Antibiogram and plasmid profiling of beta lactamase producing multi drug resistant Staphylococcus aureus isolated from poultry litter”. Journal of King Saud University, Vol. 20, pp.30200-7, 2020.
[12]. S.Y. Tong, J.S. Davis, E. Eichenberger, T.L. Holland, V.G. Fowler. “Staphylococcus aureus infections: Epidemiology, Pathophysiology, Clinical Manifestations, and Management”. Clinical Microbiology Review, Vol. 28, pp.603-661, 2015.
[13]. S. Haziz, A.S. Josiane, T.D. Victorien, A.A. Aurelle, B.M. Farid, S.B. Honor and B.M. Lamine. “Antibiotics Resistance Profile of Staphylococci Isolated from Urogenital Infections and Toxins Production of Staphylococcus aureus Strains”. Annals of Medical and Health Sciences Research, Vol. 8, Issue. 6, pp.29-34, 2018.
[14]. T. Sileshi, A. Haile, T. Admasu, T. Getachew, S.T. Tefaye, S. Workineh, E. Tadesse. “Antimicrobial resistance profile of Staphylococcus aureus isolated from patients with infection at Tikur Anbessa Specialized Hospital, Addis Ababa, Ethiopia”. BCM Pharmacology and Toxicology, Vol. 19, No. 7, pp.24-32, 2018.
[15]. A. Olowo-Okere, R.F. Atata, A. Abass, A.S. Shuaibu, U.H. Yahya, N. Tanko. “Incidence and Antibiotic Susceptibility Profile of Staphylococcus aureus Isolates from Wounds of Patients at Specialist Hospital, Sokoto, Nigeria”. Journal Medical Bacteriology, Vol. 6, No. 3, 4, pp.44-50, 2017.
[16]. A.N. Chijioke, N.N. Vivian, U.O. Christian. “Prevalence and antibiotic susceptibility pattern of Staphylococcus aureus isolated from various clinical specimens in south east Nigeria”. MOJ Cell Science & Report, Vol. 3, Issue. 2, pp.60-63, 2016.
[17]. O.A, Aboderin, A. Abdu, B.W. Odetoyin. “Antimicrobial resistance in Escherichia coli strains from urinary tract infections”. Journal of National Medical Association, Vol. 101, No. 12, pp.1268–1273, 2009.
[18]. B.A. Mincey, M.A. Parkulo. “Antibiotic prescribing practices in a teaching clinic: Comparison of resident and staff physicians”. South Medical Journal, Vol. 94, Issue. 4, pp.365–369, 2001.
[19]. S. Cowan, “Cowan and Steel manual for identification of medical bacteria”, Cambridge University Press, New York, pp.453-454, 1974.
[20]. J. Ochei, G. Kolhatkar, “Medical Laboratory Science: theory and practice”, Tata. McGraw- Hill Publishing Co. Ltd., India, pp.644-674, 2008.
[21]. T.J. Chandra, Mani, P.S. “A study of two rapid tests to differentiate Gram-positive and Gram-negative aerobic bacteria”. Journal of Medicine and Allied Science, Vol. 1, No. 4, pp.84-85, 2011.
[22]. CLSI. “Performance standards for antimicrobial susceptibility testing; Twenty-first informational supplement”. CLSI document M100-S21.Wayne, PA: Clinical and Laboratory Standards Institute, pp. 30-1 and 15, 2011.
[23]. B. Shiferaw, B. Gelaw, A. Assefa, Y. Assefa, Z. Addis. “Bacterial isolates and their antimicrobial susceptibility pattern among patients with external ocular infections at Borumeda hospital, Northeast Ethiopia”. BMC Ophthalmology, Vol. 15, Issue. 6, pp.103-109, 2015.
[24]. A.M. Sunday, A.G. Yaqub, D.M. Gideon, O.E. Godwin. “Carriage, antibiotic susceptibility and beta-lactamase production profiles of coagulase-positive Staphylococcus aureus isolated from chickens in north-eastern Nigeria”. Annals of Clinical and Medical Microbiology, Vol. 4, No. 1, pp.1022-1026, 2019.
[25]. A. Gaurav, K. Amrit, T. Roshan, K.C. Mahesh, T. Reshma. “Antimicrobial resistance patterns and plasmid profiles of methicillin-resistant Staphylococcus aureus isolated from clinical samples”. Nepal Journal of Biotechnology, Vol. 7, Issue. 1, pp.8-14, 2019.
[26]. D. Muluye, Y. Wondimeneh, G. Ferede, T. Nega, K. Adane, B. Biadgo, H. Tesfa, F. Moges. “Bacterial isolates and their antibiotic susceptibility patterns among patients with pus and/or wound discharge at Gondar university hospital”. BMC Research Notes, Vol. 7, No. 3, pp.619-625, 2014.
[27]. S. Deyno, A. Toma, Worku, M. Worku, M. Bekele. “Antimicrobial resistance profile of Staphylococcus aureus isolated from ear discharges of patients at University of Hawassa Comprehensive Specialized Hospital”. BMC Pharmacology and Toxicology, Vol. 18, No. 1, pp.35-40, 2017.
[28]. H.F. Wertheim, D.C. Melles, M.C. Vos, W. van Leeuwen, A. van Belkum, H.A. Verbrugh, J.L. Nouwen. “The role of nasal carriage in Staphylococcus aureus infections”. Lancet Infectious Diseases, Vol. 5 Issue. 12, pp.751-62, 2005.
[29]. H. Dahshan, A.M. Abd-Elall, A.M. Megahed, M.A. Abd-Elkader, E.E. Nabawy. “Veterinary antibiotic resistance, residues and ecological risks in environmental samples obtained from poultry farms”. Egyptian Environmental Monitoring Assessment, Vol. 187, No. 5, pp.1007-1014, 2015.
[30]. C. Esimone, I. Iroha, E. Ibezim, C. Okeh, E. Okpana. “In vitro evaluation of the interaction between tea extracts and penicillin G against Staphylococcus aureus”. African Journal of Biotechnology, Vol. 5, No. 11, pp.254-260, 2006.
[31]. A.O. Summers. “Generally overlooked fundamentals of bacterial genetics and ecology”. Clinical Infectious Diseases, Vol. 34 Issue. 3, pp. S85-S92, 2002.
[32]. B.S. Naseer, Y. Jayaraj. “Identification of multi-resistant Staphylococcus aureus in clinical specimens”. Journal of Resource Medicine and Science, Vol. 4, Issue. 3, pp.204-207, 2010.
[33]. A. Pitkala, L. Salmikivi, P. Bredbacka, A.L. Myllyniemi, M.T. Koskinen. “Comparison of tests for detection of beta-lactamase-producing Staphylococcus aureus”. Journal of Clinical Microbiology, Vol. 45, No. 5, pp.2031-2033, 2007.
[34]. A. Kahsay, A. Mihret, T. Abebe, T. Andualem. “Isolation and antimicrobial susceptibility pattern of Staphylococcus aureus in patients with surgical site infection at Debre Markos referral hospital, Amhara region, Ethiopia”. Archives of Public Health, Vol. 72, No. 1, pp.16-21, 2014.
[35]. W. Mulu, G. Kibru, G. Beyene, M. Damtie. “Postoperative nosocomial infections and antimicrobial resistance pattern of bacteria isolates among patients admitted at Felege Hiwot referral hospital, Bahirdar, Ethiopia”. Ethiopian Journal of Health Science, Vol. 22, Issue. 1, pp.7-18, 2012.
[36]. J.S. Harrop, J.C. Styliaras, Y.C. Ooi, K.E. Radcliff, A.R. Vaccaro, C. Wu. “Contributing factors to surgical site infections”. Journal of American Academy and Orthopaedic Surgery, Vol. 20, No. 2, pp.94-101, 2012.
[37]. H. Salimnia, W. Brown. “Detection of oxacillin resistance in Staphylococcus aureus: comparison of phoenix oxacillin and cefoxitin MICs, microscan oxacillin MIC, oxacillin and cefoxitin disk diffusion, and mecA gene detection”. ICAAC. pp.12-20, 2005.
[38]. M. Storrs, P.Courvalin, T. Foster. “Genetic analysis of gentamicin resistance in methicillin-and gentamicin-resistant strains of Staphylococcus aureus isolated in Dublin hospitals”. Antimicrobial Agents and Chemotherapy, Vol. 32, No. 8, pp.1174-1181, 2010.
[39]. Z.A. Hassam, E.J. Shaw, R. Shooter, D. Caro. “Changes in antibiotic sensitivity in strains of Staphylococcus aureus”. British Medical Journal, Vol. 2, No. 6136, pp.536-537, 2013.