Activity of Silver Nanoparticles Synthesized From Buttermilk Filtrate and Activity of Spices against Dental Cavity Causing Organisms

Prasad Memane¹ , Smita Patil² , Ashwini Sarje³

Section:Research Paper, Product Type: Journal-Paper
Vol.8 , Issue.4 , pp.80-85, Aug-2021

Online published on Aug 31, 2021

Copyright © Prasad Memane, Smita Patil, Ashwini Sarje . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
Bacterial oral pathogens can cause severe diseases with complications, severe soft tissue invasions, toxic syndromes and also cause infective endocarditis. This research paper provides comprehensive view on the silver nanoparticle synthesis from buttermilk filtrate, its characterization and its antimicrobial activity against dental pathogens. Also some spices was used to check their antimicrobial potential. The dental cavity causing organisms were isolated from patients having dental cavity problems. These organisms were identified as streptococcus spp. and candida spp. by performing morphological, cultural and biochemical characteristics referring to the bergey’s manual of determinative bacteriology. Silver nanoparticles were synthesized using buttermilk filtrate and characterization were done by UV-Vis spectroscopy and scanning electron microscopy. Extract of different spices as well as biologically synthesized silver nanoparticles were checked against identified clinical isolates by well diffusion method. Nanoparticles synthesized using buttermilk filtrate were more effective as compared to crude extracts against pathogenic strains of bacteria.

Key-Words / Index Term :
Buttermilk, Silver nanoparticles(AgNPs), streptococcus spp., candida spp., UV-Vis spectroscopy, SEM, Cinnamomum verum(cinnamon), Curcuma longa(turmeric).

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