Microbial Inhibitory Potency of Oven-Dried Mechanically Extracted Neem Seed Oil for Ecofriendly Pest Control in Food System

R.E. Nnam¹ , V.C. Onyemachi² , N.U. Okoro³ , I.S.E. Nwaorgu⁴

Section:Research Paper, Product Type: Journal-Paper
Vol.9 , Issue.2 , pp.35-43, Apr-2022

Online published on Apr 30, 2022

Copyright © R.E. Nnam, V.C. Onyemachi, N.U. Okoro, I.S.E. Nwaorgu . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
This research evaluated the anti-microbial inhibitory potency of oven-dried neem seed oil expressed mechanically for use as agricultural pest controller. The neem seed was evaluated for its moisture content and eco system friendliness. The moisture of the seed when evaluated is 9.07% which shows that high level bio-active compounds will be extracted when the oil is expressed. The ash content value is 0.133%. This shows that the neem seed oil has a negligible negative impact on the environment. The value of the oil yield mechanically expressed from neem seed oil in the research is 38.9%. The neem oil was expressed from the neem seed mechanically using a hydraulic press. The oil obtained was formulated into four concentrations of 25%, 50%, 75% and 100%. Each concentration was tested on four fungi species and four bacteria species. The fungi evaluated were A. flavus, A. niger, P. notaium and Rhizopus nigricans while the bacteria evaluated included Escherichia coli, B. subtilis, R. meliloti and Pseudomonas aeruginosa. From the mean results, the mean growth for both fungi and bacteria reduced as concentration increased. It was observed that A. flavus was completely inhibited as the percentage inhibition was 100% at 100% concentration of the neem seed oil. A. niger, P. notatium and Rhizopus nigricans recorded 96.06% 90.35% and 90.50%, respectively for 100% concentration. On the aspect of bacteria, complete inhibition was recorded for Bascillus subtilus and Pseudomonas aeruginosa while E. coli and R. meliloti recorded 96.27% and 97.28%, respectively at 100% concentration.

Key-Words / Index Term :
Neem extract, Mechanical extraction, Percentage yield, Ash content, Microbial activities, Pest control, Food system

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