Resistance Profile and Plasmid Characteristics in MDR Escherichia Coli Isolates from Patients with Gut and Urinary Tract Infections in Lafia, Nigeria

Olukayode Olugbenga OROLE¹ , Enoh Chongs MANTU² , Jebes Lamini NGOLO³ , Najaatu HADI SHEHU⁴

Section:Research Paper, Product Type: Journal-Paper
Vol.9 , Issue.3 , pp.57-61, Jun-2022

Online published on Jun 30, 2022

Copyright © Olukayode Olugbenga OROLE, Enoh Chongs MANTU, Jebes Lamini NGOLO, Najaatu HADI SHEHU . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
Escherichia coli is a prevalent microorganism responsible for gastrointestinal (GIT) and urinary tract infections (UTIs) in patients attending health facilities all over the world. The study determined the plasmids profile of multi-drug resistant (MDR) E. coli from urine and fecal samples. Of the 300 samples analyzed using microbiological techniques, E. coli identified were screened for antibiotic susceptibility, and the plasmid DNA of resistant isolates was extracted and amplified on PCR. Obtained E. coli were mostly resistant to ampicillin (79.2%) in stool and 82.0% in urine samples while imipenem had the best activity against the bacterium 18.0% (urine) and 9.4% in stool samples. Fifteen resistance patterns were obtained from the different classes of antibiotics tested against E. coli from the stool and 14 from the urine samples. Isolates U15 and U16 had 3 plasmids each 7000, 4200, 2500, 10000, 3500, and 2000 respectively to make them resistant isolates with the highest number. The study concluded that E. coli from fecal and urine samples of patients presenting infections of gastrointestinal and urinary tracts in Lafia, Nigeria contains plasmid DNA responsible for the multi-drug resistance.

Key-Words / Index Term :
Antibiotics, genes, infections, plasmid, virulence

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