Isolation and Identification of Some Bacteria That Cause Burns and Used Aloe Vera Extract As an Antibacterial Agent

Zaman S. Hamza¹ , Zahraa Mohammed Yahya² , Raghad Obead AbdulAL-Abass³

Section:Research Paper, Product Type: Journal-Paper
Vol.10 , Issue.5 , pp.29-35, Oct-2023

Online published on Oct 31, 2023

Copyright © Zaman S. Hamza, Zahraa Mohammed Yahya, Raghad Obead AbdulAL-Abass . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

View this paper at   Google Scholar | DPI Digital Library

XML View     PDF Download

How to Cite this Paper

Abstract :
This research sought to identify the bacterial agents responsible for burn and wound infections. A total of 30 swab samples from burn victims of both sexes and of different ages as well as 7 swab samples from healthy people were gathered for these purposes. Patients were transferred to the Specialized Center for Burns-Najaf and Imam Sadiq Hospital, respectively. samples have been cultured on the MacConkey agar, nutrition agar, blood agar, and EMB agar as a preliminary step in the process of bacteria cultivation. Thirty bacterial isolates were discovered, according to the findings. They were determined to be members of the following 17 species of negative bacteria after being identified by cultural, microscopic, and biochemical characterizations: 10 different Klebsiella pneumoniae, 3 different E. coli species, and Pseudomonas aeruginosa. after subjecting these isolates to the well-diffusion agar techniques` susceptibility test against Aleo vera suspension at various concentrations (50, 100, 100, 150, and 200 L). In this investigation, it was discovered that as the concentration of Culture supernatans in the well grew, so did the growth inhibition. demonstrated that 200 L higher doses totally stopped the development of all of these bacteria and that 50 L of Aleo vera suspension had a mild inhibitory effect on all bacterial growth. Additionally, these findings demonstrate that E. coli has a 200-fold higher inhibitory efficiency than other bacteria, but Klebsiella pneumoniae has a poor inhibition zone at the same dose. It`s interesting.

Key-Words / Index Term :
Klebsiella pneumonia, Aleo vera , burns , antibiotics , MIC

References :
[1] Seyyed Jalil Mirmohammadi ., Amir Houshang Mehrparvar .,Kazem Kazemeini and Mehrdad Mostaghaci , "Epidemiology characteristics of occupational burns in Yazd, Iran " , International Journal of Preventive Medicine. voI. 4 , issue . 6 , pp 723–727, 2013.
[2] Hardisiswo Soedjana , J Nadia , Sundoro A, Lisa Hasibuan , Hardisiswo Soedjana , Almahitta cintami Putri , Rani Septrina , Betha Riestiano , Arif Tri Prasetyo and S Harianti. "The Profile Of Severe Burn Injury Patients With Sepsis In HasanSadikin Bandung General HospitaL". "Ann Burns Fire Disasters". , voI, 33 , issue . 4 , pp . 312-316 , 2020.
[3] Eduardo Camacho Quintero , Jesús Francisco Escrivá Machado and Roger Andres Damian Robles . " Meek micrografting history, indications, technique, physiology and experience: a review article.J Wound Care " .voI .27 , issue .2 , pp . 12- 18, 2018 .
[4] Nicolas Germain ,Anne-Sophie Hatzfeld , Louise Pasquesoone , Pierre-Marie Danze , Pierre Guerreschi , Boualem Sendid , Olivier Gaillot and Philippe Marchetti , "How to improve donor skin availability: Pragmatic procedures to minimize the discard rate of cryopreserved allografts in skin banking. Burns ". voL. 47 , issue . 2 , pp . 387-396, 2021.
[5] Luis Cobrado , Ana Silva-Dias , Azevedo MM, Rodrigues AG. High-touch surfaces: microbial neighbours at hand. Eur J ClinMicrobiol Infect Dis.; voI.36 , issue .11 , pp 2053-2062 , 2017 .
[6] Abid Rashid , Muhammad Saqib , Farrah Deeba , Junaid Ali Khan . "Microbial profile of burn wound infections and their antibiotic sensitivity patterns at burn unit of allied hospital Faisalabad " . "Pak J Pharm Sci", voI. 32 , issue . 1, pp . 247-254, 2019 .
[7] Kaisen Chen , Shirong Lin , Peiqun Li , Qiuyue Song , Dong Luo , Tao Liu , Lingbing Zeng and Wei Zhang . "Characterization of Staphylococcus aureus isolated from patients with burns in a regional burn center, Southeastern China" , BMC Infect Dis., 25; voI . 18 , issue . 1 , pp 50-51, 2018 .
[8] Clinical and Laboratory Standards Institute, Performance Standards for Antimicrobial Susceptibility Testing: Twenty Second Informational Supplement, M100-S20, CLSI, Wayne, Pa, USA, 2012.
[9] Ingrida Januleviciene , Lina Siaudvytyte . and Ruta Barsauskaite ."Ophthalmic Drug Delivery in Glaucoma- A review. Pharmaceutics" , voI 4 , issue 1 , pp. 243-251, 2012 .
[10] Adnan,M.J., Hameedah H.Al-Ahbabi , Rabab Alhussani and A. Hamad , "Study of the efficacy of Aloe vera extracts in treatment of non-infected wounds induced by sulferric acid and infected wounds with Staphylococcus aureus". International Journal of Advanced Research, voI . 3 , issue. 1, pp. 593- 601, 2015 .
[11] Seyyed Abbas Hashemi., Seyyed Abdollah Madani . and Saied Abediankenari . " The Review on Properties on Properties of Aloe vera in Healing of the Cutaneous Wounds ". BioMed Research International, pp . 1-6 , 2015
[12] Yebpella G. Gary , Chrinius Hammuel., M. M. Adeyemi ., Asabe Mercy Magomya., Abel S Agbajp . and Gideon Adamu Shallangwa . "Phytochemical screening and a comparative study of antibacterial activity of Aloe vera green rind, gel and leaf pulp extracts".International Research Journal of Microbiology, voI . 2 , issue . 10 , pp. 382-386, 2011.
[13] Jothi Karumari R., Vijayalakshmi, K ., Tamilarasi, L. and Ezhilarasi Balasubramanian ." Antibacterial activity of leaf extracts of Aloe vera, Ocimum sanctum and Sesbaniagrandiflora against the gram positive bacteria" , International Journal of Biomedical and Pharmaceutical Sciences , voI. 4 , issue . 35 , pp. 60-63, 2014 .
[14] Maharjan H Radha and Nampoothiri P Laxmipriya, “Evaluation of biological properties and clinical effectiveness of Aloe vera: a systematic review,” Journal of Traditional and Complementary Medicine, vol. 5, no. 1, pp. 21–26, 2015.
[15] Mbajiuka Chinedu Stanley , Obeagu Emmanuel Ifeanyi, and Okwandu GodsonEziokwu, “Antimicrobial effects of Aloe vera on some human pathogens,” International Journal of Current Microbiology and Applied Sciences, vol. 3, issue. 3, pp. 1022–1028, 2014.
[16] Reza Kazemi Darsanaki , Mahdiyeh Laleh Rokhi .;Morteza Azizollahi Aliabadi and Khosro Issazadeh , "Antimicrobial Activities of Lactobacillus Strains Isolated from Fresh Vegetables". Middle-East Journal of Scientific Research., voI . 11, issue .9 , pp. 1216-1219, 2012 .
[17] David B. Duncan . "Multiple Range and Multiple F Tests". Biometrics. Vol. 11, Iss: 4, pp 1-42 ,1955.
[18] Nadeem Sajjad Raja and Nishi Nihar Singh . "Antimicrobial susceptibility pattern of clinical isolates of Pseudomonas aeruginosa in tertiary care hospital" . J Microbiol Immunol Infect ,. voI . 40 , issue .1 , pp . 45-49 , 2007 .
[19]- Alireza Ekrami. and Enayat Kalantar. "Bacterial infections in burn patients at a burn hospital in Iran" . Indian J. Med. Res. voI . 126 , issue . 6 , pp . 541-544, 2007 .
[20]- Mohammed J. Alwan , Inam Jasim Lafta . and Aseel M. Hamzah ,. "Bacterial isolation from burn wound infections and studying antimicrobial susceptibility". Kufa Journal for Veterinary Medical Sciences , voI . 2 , issue .1 , pp . 121-131, 2011
[21] Japoni A, Shohreh Farshad and Alborzi Abdolvahab ,“Pseudomonas aeruginosa: burn infection, treatment and antibacterial resistance,” Iranian Red Crescent Medical Journal, vol. 11, issue. 3, pp. 244–253, 2009.
[22] Mohammadmehdi Fani and amshid Kohanteb “Inhibitory activity of Aloe vera gel on some clinically isolated cariogenic and periodontopathic bacteria,” Journal of Oral Science, vol. 54, issue 1, pp. 15–21, 2012.
[23] Farida Anjum and Asif Mir , "Susceptibility of Pseudomonas aeruginoas against various antibiotics" . Afr. J. Microbiol.Res. voI .4 , issue .10 , pp . 1005- 1012 , 2010 .

[24] Manuel R. Gonzalez , Betty Fleuchot , Leonardo Lauciello , Paris Jafari , Lee Ann Applegate , Wassim Raffoul , et al . "Effect of Human Burn Wound Exudate on Pseudomonas aeruginosa Virulence" . mSphere. , voI . 1 , issue . 2 , pp 11- 15 , 2016.
[25] Adane Bitew, Tamirat Molalign and Meseret Chanie , "Chanie M . Species distribution and antibiotic susceptibility profile of bacterial uropathogens among patients complaining urinary tract infections." BMC Infect Dis. voI. 17 , issue . 1 , pp . 6-54 . 2017.
[26] Kunle, Oluyemisi Folashade , Egharevba, Henry Omoregie ,and Ahmadu, Peter Ochogu, “Standardization of herbal medicines—a review,” International Journal of Biodiversity and Conservation, vol. 4, issue. 3, pp. 101–112, 2012.
[27]Nejatzadeh-Barandozi, “Antibacterial activities and antioxidant capacity of Aloe vera,” Organic and Medicinal Chemistry Letters, vol. 3, no. 5, pp. 1–8, 2013.
[ 28] Richa Tiwari, Geetika Bakshi, Niranjan Tiwari and Nitin Puranik, "Production, Purification and efficacy determination of Epsilon toxin from Clostridium perfringens type ‘D’ IVRI native culture," International Journal of Scientific Research in Biological Sciences, Vol.2, Issue.2, pp.1-4, 2015
[29] Smriti Chitnis and Uday Chitnis, "Pollen Production Studies in Some Trees Growing at Bhopal, M.P.," International Journal of Scientific Research in Biological Sciences, Vol.2, Issue.3, pp.1-4, 2015.