Estimation of Diosgenin by HPLC in a Dioscorea zingiberensis Collection from Different Local Markets in Middle and South Iraq

Zahraa A.N. Al-Yassiry¹ , Mohammed Abdullah Jebor² , Basheer Al-Alwani³

1. Dept. of biology /Faculty of science, University of Babylon, Babel, Iraq.
2. Dept. of biology /Faculty of science, University of Babylon, Babel, Iraq.
3. Dept. of biology /Faculty of science, University of Babylon, Babel, Iraq.

Section:Research Paper, Product Type: Journal-Paper
Vol.11 , Issue.4 , pp.15-19, Aug-2024

Online published on Aug 31, 2024

Copyright © Zahraa A.N. Al-Yassiry, Mohammed Abdullah Jebor, Basheer Al-Alwani . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
Diosgenin, a steroidal sapogenin, is found in significant quantities in some plant species, such as Dioscorea alata, Smilax China, and Trigonella foenum graecum. The bioactive phytochemical discussed in this context serves as a crucial precursor in the pharmaceutical sector for the synthesis of numerous steroidal medications. Additionally, it has demonstrated significant promise and garnered considerable attention for its potential therapeutic applications in addressing a range of disorders, including cancer, hypercholesterolemia, inflammation, and various infections. The objective of this research was to assess the diosgenin concentration in Dioscorea samples obtained from several local markets by the utilization of High Performance Liquid Chromatography (HPLC). The findings indicated that the content of Diosgenin was determined within the range assessed using the standard diosgenin. The utilization of high-performance liquid chromatography (HPLC) for the quantitative assessment of diosgenin has been determined to be a rapid, precise, straightforward, and cost-efficient alternative method for the examination of diosgenin.

Key-Words / Index Term :
Diosgenin, HPLC , Dioscorea , bioprospection , plant , physiology

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