A Study in the Viability of Butea monosperma Seeds

Anupama Modi¹ , Anand Kandya²

1. Department of Botany , Shri Umiya Kanya Mahavidalay, Indore, India.
2. Department of Botany, Dr. Hari Singh Gour University, Sagar, India.

Correspondence should be addressed to: dranupamamodi@gmail.com.

Section:Survey Paper, Product Type: Isroset-Journal
Vol.4 , Issue.6 , pp.8-10, Dec-2017

CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v4i6.810

Online published on Dec 31, 2017

Copyright © Anupama Modi, Anand Kandya . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
Tetrazolium testes are employed to evalute seed viability so as to achieve faster as well as reliable results. The Tetrazolium Test involves the use of 2, 3, 5, triphenyl tetrazolium chloride which in a living cell is reduced by the dehydrogenase enzyme system. This reaction yields a red compound called triphenyl formazan. Since this reaction is enzyme mediated the cell in which it is taking place has to be a liveing cell. Stained areas of a tissue in this test indicate occurrence of live cells and unstained ones the necrotic tissue. Thus, the test is considered very reliable to locate living cells. In evaluation of seed viability occurrence of a definite proportion of necrotic tissue to that of live tissue helps to the seed will germinate or not.

Key-Words / Index Term :
Tetrazolium, Enzyme, Living, Non-living tissue, germination and staining

References :
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