3D structure prediction of OCT 4, an important Reprogramming Factor of induced Pluripotent Stem Cells (iPSCs)

P.Chauhan ¹ , N. Wal²

1. Department of Microbiology, Mewar University, Gangrar, Chittorgarh, Rajasthan, India.
2. Department of Microbiology, Mewar University, Chittorgarh, Rajasthan, India.

Section:Research Paper, Product Type: Isroset-Journal
Vol.5 , Issue.2 , pp.1-6, Apr-2018

CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v5i2.16

Online published on Apr 30, 2018

Copyright © P.Chauhan, N. Wal . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
Oct 4 is one of the transcription factors among six reprogramming factors (OCT4, SOX2, KLF4, C-MYC, NANOG, and LIN28) selected by Takahashi and Yamanaka to induce somatic cells into pluripotent stem cells (iPSCs).Stem cell research is used in treatment of a number of diseases including genetic disorders. Several questions regarding reprogramming factors of stem cells are remaining unanswerable due to limited experimental availability and ehilical issues. Proteomic analysis of OCT 4 is still remaining unpredicted as protein structure is not available in PDB. The aim of this study was prediction of the tertiary structure of OCT4 protein using homology modeling approach through MODELLER program. Quality and reliability assessments were performed on predicted model and found the model reliable.

Key-Words / Index Term :
iPSCs, therapeutic targets, homology modeling, template, reprogramming factors, OCT 4

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