Isolation of Mycobacteriophage: Novel tool to treat Mycobacterium spp

R. Satish¹ , A. Desouza²

1. Department of Microbiology, SIES College of Arts, Science &Commerce, Sion(west), Mumbai University, Mumbai, India.
2. Department of Microbiology, SIES College of Arts, Science &Commerce, Sion(west), Mumbai University, Mumbai, India.

Correspondence should be addressed to: rajithasatishb@gmail.com.

Section:Research Paper, Product Type: Isroset-Journal
Vol.5 , Issue.2 , pp.46-50, Apr-2018

CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v5i2.4650

Online published on Apr 30, 2018

Copyright © R. Satish, A. Desouza . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
Mycobacteriophages are viruses that infect Mycobacterium spp. To date,9666 mycobacteriophages have been isolated and 1519 mycobacteriophage genomes have been sequenced(phagesdb.org). The aim of this study was to isolate mycobacteriophages from different soil samples using Mycobacterium smegmatis as host. In this study mycobacteriophages have been isolated from 10 different soil samples. Presence of these phages was confirmed by qualitative plaque formation on plates. These 10 different phages were further tested for host diversity using M. fortuitum subsp. fortuitum MTCC993, Mycobacterium kansasiiMTCC3058, Mycobacterium aviumsubsp. avium MTCC1723 and Mycobacterium tuberculosis MTCC300. Three among these 10 phages, were found to infect all the 4 different species of Mycobacterium besides the host Mycobacterium smegmatis that was used for isolation of the phages. This truly reflects the host diversity of the phages and their ability to rapidly adapt to new hosts. These phages could also hold a great potential to be used as tools of genetic manipulation to study Mycobacteria. Their potential for the treatment and eradication of M. tuberculosis can also be studied further.

Key-Words / Index Term :
Mycobacteriophages, Mycobacterium tuberculosis, Mycobacterium smegmatis, MDR-TB, Host diversity

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