Optimization and Characterization of Xylanase from An Indigenous Strain of Aureobasidium Pullulans

Deepak K. Rahi¹ , Richa ² , Maninderjeet Kaur³

1. Department of Microbiology, Panjab University, Chandigarh, India.
2. Department of Microbiology, Panjab University, Chandigarh, India.
3. Department of Microbiology, Panjab University, Chandigarh, India.

Section:Research Paper, Product Type: Isroset-Journal
Vol.5 , Issue.3 , pp.72-79, Jun-2018

CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v5i3.7279

Online published on Jun 30, 2018

Copyright © Deepak K. Rahi, Richa, Maninderjeet Kaur . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
Xylanases are versatile hydrolytic enzymes in nature, due to its degradatory properties and ability to degrade hemicelluloses efficiently. Xylanases and associated debranching enzymes produced by a variety of microorganisms including bacteria, actinomycetes, yeast and fungi bring hydrolysis of hemicelluloses. Among microorganisms, filamentous fungi are the predominant source organism for xylanases. Xylanases have fortified awesome interest because of their potential application in a many industries. Throughout the years, the quantity of conceivable uses of xylanases in the paper and pulp industry has expanded relentlessly, and a few applications have been popularized. In present investigation an indigenous species of Aureobasidium pullulans was evaluated for xylanse producing efficiency and various parameters affecting the production was standardized employing one-factor at a time approach. This resulted in significant enhancement of enzyme activity from 8.7 U/ml to 27.75 U/ml. The enzyme optima for pH and temperature were found to be 6 and 55 °C respectively.

Key-Words / Index Term :
Xylanase, Aurobasidium pullulans, optimization, characterization

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