Effects of soybean meal incorporated diet on enzyme producing autochthonous gut bacteria in fingerlings of Labeo rohita (Hamilton)

Pinki Ghosh¹ , Suhas K. Dan² , Goutam Banerjee³ , Sandip Majumder⁴ , Ankita Nandi⁵ , Kousik Roy⁶ , Arun K. Ray⁷

Section:Research Paper, Product Type: Isroset-Journal
Vol.5 , Issue.4 , pp.47-54, Aug-2018

CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v5i4.4754

Online published on Aug 31, 2018

Copyright © Pinki Ghosh, Suhas K. Dan, Goutam Banerjee, Sandip Majumder, Ankita Nandi, Kousik Roy, Arun K. Ray . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
Gut microbiota have important role in the digestive process of fish. Soybean meal based diet effects on gut microbiota of Labeo rohita by altering enzymatic activities like amylase, protease and cellulase. Two diets were prepared, one using fish meal and the other using soybean meal as the major protein source. The feeding trial was conducted in 90L circular flow-through fibre-glass tanks for 90 days under laboratory condition. After ninety days of feeding trial fish weight gain percentage, SGR FCR, PER was better with soybean meal diet in comparison to the fish meal diet. Amylase, cellulase and protease producing aerobic bacterial count were increased in both the proximal intestine (PI) and distal intestine (DI). The isolated strains from the PI and DI of the test fish were screened by qualitative enzyme test. The specific amylase, cellulase and protease activity before and after feeding trial were recorded. A distinct change in the quantitative enzyme activities in the bacterial strains were noticed after feeding with soybean meal diets which indicated that soybean meal can influence the autochthonous enzyme-producing microbial community in the GI tract of fish.

Key-Words / Index Term :
Gut bacteria, Soybean meal, Enzyme activity, Labeo rohita, Growth

References :
[1] D.A. Higgs, B.S. Dosanjh, A.F. Prendergast, R.M. Beams, R.W. Hardy, W. Riley, G. Deacon, “Nutrition and Utilization Technology in Aquaculture”, Champaign II. Chapman and Hall Company, New York, USA, pp. 130-156, 1995.
[2] A.G.J. Tacon, "Feed ingredients for warmwater fish, fish meal and other processed feedstuffs", FAO Fisheries Circular (FAO). no. 856, 1993.
[3] T O’keefe, “Plant protein ingredients for aquaculture feeds: Use considerations and quality standards”, Aquaculture Seminars, 24-28 July, 2003. Mymensingh-Khulna- Dhaka, Bangladesh, 14 p. 2003.
[4] A.K. Ray, K. Ghosh, E. Ringø, “Enzyme-producing bacteria isolated from fish gut: A review”, Aquaculture Nutrition 18, 465-492, 2012a.
[5] F. Askarian, S. Sperstad, D.L. Merrifield, A.K. Ray, E. Ringø, “The effect of different feeding regimes on enzyme activity of Atlantic cod (Gadus morhua L.) gut microbiota” Aquaculture Research, 44, 841-846, 2013.
[6] E. Ringø, E. Strøm, "Microflora of Arctic charr, Salvelinus alpinus (L.): gastrointestinal microflora of free‐living fish and effect of diet and salinity on intestinal microflora", Aquaculture Research, 25(6), 623-629, 1994.
[7] K.M. Das, S.D. Tripathi, “Studies on the digestive enzymes of grass carp, Ctenopharyngodon idella (Val.)”, Aquaculture, 92, 21-32, 1991.
[8] G. Banerjee, A.K. Ray, F. Askarian, E. Ringø, “Characterisation and identification of enzyme-producing autochthonous bacteria from the gastrointestinal tract of two Indian air-breathing fish”, Beneficial Microbes, 4, 277-284, 2013.
[9] O.H. Lowry, N.J. Rosebrough, A.L. Farr, R.J. Randall, “Protein measurement with the folin phenol reagent”, Journal of biological chemistry, 193, 265-275, 1951.
[10] P. Bernfeld, “Amylase [alpha] and [beta]. In: Kolowick SP, Kaplan NO (Eds.) Methods of enzymology”, Academic Press, New York, NY, USA, pp. 149-150, 1955.
[11] D.A. Denison, R.D. Koehn, “Cellulase activity of Poronia Oedipus”, Mycologia, 69, 592-601, 1977.
[12] H.E. Walter, “Methods of enzymatic analysis”, Verlag Chemie, Weinheim, Germany, 1984.
[13] T.H. Birkbeck, E. Ringø, “Pathogenesis and the gastrointestinal tract of growing fish”, In: W Holzapfel, P.Naughton, (Eds.), Microbial Ecol Growing Ani, Elsevier, Edinburgh, UK, pp. 208-234, 2005.
[14] E. Ringø, U Schillinger, W. Holzapfel, “Antibacterial abilities of lactic acid bacteria isolated from aquatic animals and the use of lactic acid bacteria in aquaculture”, In: Holzapfel W, Naughton P (Eds.) Microbial Ecology in Growing Animals, Elsevier, Edinburgh, pp. 418-453, UK 2005.
[15] K. Korsnes, O. Nicolaisen, C.K. Skår, A.H. Nerland, Ø. “Bergh, Bacteria in the gut of juvenile cod Gadus morhua fed live feed enriched with four different commercial diets”, ICES Journal of Marine Science: Journal du Conseil, 63(2), 296-301, 2006.
[16] E. Ringø, S. Sperstad, R. Myklebust, S. Refstie, Å. Krogdahl, “Characterisation of the microbiota associated with intestine of Atlantic cod (Gadus morhua L.). The effect of fish meal, standard soybean meal and bioprocessed soybean meal’, Aquaculture, 261, 829-841, 2006b.
[17] J. Heikkinen, J. Vielma, O. Kemilainen, M. Tiirola, P. Eskelinen, T. Kiuru, D. Navia- Paldanius, A. von, “Wright Effects of soybean meal based diet on growth performance, gut histopathology and intestinal microbiota of juvenile rainbow trout (Oncorhynchus mykiss)”, Aquaculture, 261, 259-268, 2006.
[18] S.K. Nayak, “Probiotics and immunity: a fish perspective” Fish and Shellfish Immunology, 29, 2-14, 2010b.
[19] C.F. Cai, W.J. Wang, T.T. Ye, Å. Krogdahl, Y.L. Wang, Y.M. Xia, C.G. Yang, “Effect of soybean meal, raffinose and stachyose on the growth, body composition, intestinal morphology and intestinal microflora of juvenile allgynogenetic silver crucian carp (Carrassius auratus gibelio♀ 9 Cyprinus carpio ♂)”, Aquaculture Ressearch, 41,128-138, 2012.
[20] D.L. Merrifield, A. Dimitroglou, G. Bradley, R.T.M. Baker, S.J. Davies, “Soybean meal alters autochthonous microbial populations, microvilli morphology and compromises intestinal enterocyte integrity of rainbow trout (Oncorhynchus mykiss Walbaum)”, Journal of Fish Diseases, 32, 755-766, 2009.
[21] E. Ringø, S. Sperstad, O.F. Kraugerud, Å. Krogdahl, “Use of 16S rRNA gene sequencing analysis to characterize culturable intestinal bacteria in Atlantic salmon (Salmo salar) fed diets with cellulose or non‐starch polysaccharides from soy”, Aquaculture Research, 39 (10),1087-1100, 2008.
[22] A.M. Bakke-McKellep, M.H. Penn, P. Mora Salas, S. Refstie, S. Sperstad, T. Landsverk, E. Ringø, Å. Krogdahl, “Effects of dietary soyabean meal, inulin and oxytetracycline on intestinal microbiota and epithelial cell stress, apoptosis and proliferation in the teleost Atlantic salmon (Salmo salar L.)”, British Journal of Nutrition, 97, 699-713, 2007.
[23] C. Jeppesen, “Media for Aeromonas spp., Plesiomonas shigelloides and Pseudomonas spp. from food and environment”, International journal of food microbiology, 26, 25-41, 1995.
[24] T.J. Donovan, P. van Netten, “Culture media for the isolation and enumeration of pathogenic Vibrio species in foods and environmental samples”, In Progress in industrial microbiology, 34, 203-217, Elsevier, 1995.
[25] E. Ringø, E, Strøm, J.L. Tabachek, “Intestinal microflora of salmonids: a review”, Aquaculture Research, 26, 773-789, 1995.
[26] D.L. Merrifield, G. Bradley, R.T. Baker, S.J. Davies, “Probiotic applications for rainbow trout (Oncorhynchus mykiss Walbaum) II. Effects on growth performance, feed utilization, intestinal microbiota and related health criteria post antibiotic treatment”, Aquaculture Nutrition, 16(5), 496-503, 2010b.
[27] S.K. Nayak, “Role of gastrointestinal microbiota in fish”, Aquaculture Research, 41, 1553-1726, 2010a.
[28] C.M. Caipang, M.F. Brinchmann, V. Kiron, “Antagonistic activity of bacterial isolates from intestinal microbiota of Atlantic cod, Gadus morhua, and an investigation of their immunomodulatory capabilities” Aquaculture Research, 41(2), 249-256, 2010.
[29] F.S. Pedrotti, S. Davies, D.L. Merrifield, M.R.F. Marques, A.P.M Fraga, J.L.P. Mouri~no, D.M. Fracalossi, “The autochthonous microbiota of the freshwater omnivores jundi_a (Rhamdia quelen) and tilapia (Oreochromis niloticus) and the effect of dietary carbohydrates”, Aquaculture Research, 46, 472-481. 2015.
[30] S. Mondal, T. Roy, S.K. Sen, A.K. Ray, “Distribution of enzyme producing bacteria in the digestive tracts of some freshwater fish”, Acta Ichthyologica et Piscatoria, 38, 1-8, 2008b.