Prevalence of blaSHV gene in Cephalosporin Resistant Salmonella Isolates from Meat Samples in South India

G Pradeep¹ , S K Mendem² , G C Math³ , C T Shivannavar⁴ , S M Gaddad⁵

Section:Research Paper, Product Type: Isroset-Journal
Vol.5 , Issue.4 , pp.82-86, Aug-2018

CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v5i4.8286

Online published on Aug 31, 2018

Copyright © G Pradeep, S K Mendem, G C Math, C T Shivannavar , S M Gaddad . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
Antibiotic resistant salmonella infections posing a serious threat global health. blaSHV genes belongs a major group of extended spectrum beta lactamases (ESBL). In the current study, Cephalosporin Resistant in Salmonella spp conformed by detecting blaSHV genes using PCR amplification. 28 cephalosporin resistant Salmonella sp (S. typhimurium, n= 22 and S. enteridis n=06) isolated from meat samples collected from various vendors in Hyderabad city, South India were subjected for phenotypic detection of ESBL production and blaSHV gene responsible for ESBL production was detected by PCR amplification. Among the 28 cephalosporin resistant Salmonella sp (CeRSsp), 4 isolates (14.2%) were positive for ceftazidime mediated ESBL production. Screening of these isolates indicated the presence of about 6 kbp plasmid. PCR amplification indicated the presence of blaSHV gene with an amplicon size of 755 bp. The presence blaSHV gene contributes for ESBL production which makes organisms to show reduced susceptibility against third generation cephalosporins.

Key-Words / Index Term :
Cephalosporin resistant Salmonella sp, ESBL, blaSHV, food-borne pathogens,

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