Nitric Oxide Scavenging Activity of Bioactive Compounds Present in Methanolic Extract of Morchella esculenta

D.R. Baba¹ , P.M. Junaid² , F. Khan³ , R. Singh⁴

1. Department of Life Sciences, Govt. M.V.M, Barkatullah University , Bhopal, India.
2. Department of Life Sciences, Govt. M.V.M, Barkatullah University , Bhopal, India.
3. Department of Botany, Govt. College Nasrullah Gung, Sehore, India.
4. Department of Life Sciences, Govt. M.V.M, Barkatullah University , Bhopal, India.

Section:Research Paper, Product Type: Isroset-Journal
Vol.5 , Issue.5 , pp.39-41, Oct-2018

CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v5i5.3941

Online published on Oct 31, 2018

Copyright © D.R. Baba, P.M. Junaid, F. Khan, R. Singh . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

View this paper at   Google Scholar | DPI Digital Library

XML View     PDF Download

How to Cite this Paper

580 Views    292 Downloads    185 Downloads

Abstract :
Morchella esculenta is commonly known as morel, common morel, true morel, yellow morel, sponge morel and morel mushroom [1]. It is one of the most easily recognized of all the edible mushrooms and highly sought after. Morchella mushroom is one of the costliest edible fungi in the world. Morchella esculenta is in economically important mushroom commonly known as “Guchhi” in India. In India, the presence of Morchella is reported from Himachal Pradesh, Uttar Pradesh and Jammu and Kashmir [2]. It has been reported that the morel species are good to minimize the oxidative damage in organisms that occur in several chronic diseases. Moreover, these morel species can be used to find new antimicrobials overlapping the resistance of bacteria to first choice antibiotics. The compounds like Phenol, Tocopherol and organic acids are considered to be the most responsible for antioxidant activity of mushrooms [3][4]. Methanol extract of Morchella esculenta was screened to evaluate free radical scavenging effect. The extract exhibited significant reducing power and free radical scavenging effect on nitric oxide radicals. This antioxidant activity was concentration dependent which were compared with standard antioxidants such as ascorbic acid. The highest free radical scavenging effect of the extract was observed at concentration of 100μg/ml and the IC50 value of extract was 49.8821μg/ml.

Key-Words / Index Term :
Nitric oxide scavenging, Morchella esculenta, Methanolic Extract, Antioxidant activity

References :
[1]. Prasad, P. K., Chauhan, L.S., Kandari, R.K., Maikhuri, A., Purohit, P. Bhatt and Rao, K. S. (2002) Morchella esculenta (Guchhi): Need for scientific intervention for its cultivation in Central Himalaya, Curr. Sci. 82(9): 1098 - 1100.
[2]. Lakhanpal, T.N., Shad, O. and Rana, M. (2010) Biology of Indian Morels. I. K. International Publishing House, New Delhi.
[3]. Reis, F.S., Stojkovic, D., Sokovic, M., Glamoclija, J., Ciric, A., Barros, L., and Ferreira, I.C.F.R. (2012). Chemical characterization of Agaricus bohusii, antioxidant potential and antifungal preserving properties when incorporated in cream cheese. Food Research International. 48: 620-626.
[4]. Leal, A.R., Barros, L., Barreira, J.C.M., Sousa, M.J., Martins, A., Santos-Buelga, C., and Ferreira, I.C.F.R. (2013). Portuguese wild mushrooms at the "Pharma-Nutrition" interface: Nutritional characterization and antioxidant properties. Food Research International. 50: 1-9.
[5]. Sachindra, N. M., M. K. W. A. Airanthi, M. Hosokawa and K. Miyashita, 2010. Radical scavenging and singlet oxygen quenching activity of extracts from Indian seaweeds. Journal of Food Science and Technology, 47: 94–99.
[6]. Kosanic, M., B. Rankovic and J. Vukojevic, 2011. Antioxidant properties of some lichen species. Journal of Food Science and Technology, 48: 584-590.
[7]. Zhang, W. M., B. L. Lin Han and H. D. Zhang, 2009. Antioxidant activities of extracts from Areca (Areca catectu L.) flower, husk and seed. Electronic Journal of Environmental, Agricultural and Food Chemistry, 8: 740-748.
[8]. Oyetayo, V. O., C. H. Dong and Y. J. Yao, 2009. Antioxidant and Antimicrobial Properties of Aqueous Extract from Dictyophora indusiata. The Open Mycology Journal, 3: 20-26.
[9]. Mau, J. L., C. N. Chang, S. J. Huang and C. C. Chen, 2004. Antioxidant properties of methanolic extract from Grifola frondosa, Morchella esculenta and Termitomyces albuminosus mycelia. Food Chemistry, 87: 111-118.
[10]. Barros, L., R. C. Calhelha, J. A. Vaz, I. C. F. R. Ferreira, P. Baptista and L. M. Estevinho, 2007. Antimicrobial activity and bioactive compounds of Portuguese wild edible mushrooms methanolic extracts. European Food Research and Technology, 225: 151-156.
[11]. Ferreira, I. C. F. R., P. Baptista, M. Vilas-Boas and L. Barros, 2007. Free-radical scavenging capacity and reducing power of wild edible mushrooms from northeast Portugal: individual cap and stipe activity. Food Chemistry, 100: 1511-1516.
[12]. Jagetia G.C, Rao S.K, Baliga M.S, Babu K.S. The Evaluation of Nitric Oxide Scavenging Activity of Certain Herbal Formulations in vitro: A Preliminary Study. Phytother. Res. 18: 561–565, 2004.
[13]. Vriesman M.F, Haenen G.R, Westerveld G.J, Paquay J.B, Voss H.P, Bast A. A method for measuring nitric oxide radical scavenging activity. Scavenging properties of sulfur-containing compounds. Pharm World Sci. 19(6): 283-286, 1997.
[14]. Hagerman A.E, Riedl K.M, Jones G.A, Sovik K.N, Ritchard N.T, Hartzfeld P.W. 1998. High molecular weight plant poly-phenolics (tannins) as biological antioxidants. J Agric and Food Chem. 46: 1887-1892, 1998.
[15]. Nabavi SM, Ebrahimzadeh M.A, Nabavi S.F, Hamidinia A, Bekhradnia A.R. Determination of antioxidant activity, phenol and flavonoids content of Parrotia persica Mey. Pharmacology online 2: 560-567, 2008.
[16]. Gulati K, Ray A, Masood A, Vijayan V.K. Involvement of nitric oxide (NO) in the regulation of stress susceptibility and adaptation in rats. Indian J Exp Biol. 44:809-815, 2006.