Elementary flux mode analysis of central carbon metabolism in Caldicellulosiruptor saccharolyticus to enhance hydrogen production

Nupoor Chowdhary¹ , Gopal Ramesh Kumar²

Section:Research Paper, Product Type: Isroset-Journal
Vol.5 , Issue.5 , pp.108-116, Oct-2018

CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v5i5.108116

Online published on Oct 31, 2018

Copyright © Nupoor Chowdhary, Gopal Ramesh Kumar . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
Microbial hydrogen production from biomass has been perceived as an imperative wellspring of renewable energy. Caldicellulosiruptor saccharolyticus has proven itself to be an excellent candidate for biological hydrogen production. Hydrogen is produced as a biological product from central carbon metabolism pathway by the utilization of various soluble sugar substrates. An organism’s metabolism is a key factor in understanding its physiology, therefore, metabolic network of central carbon metabolism of C. saccharolyticus was reconstructed. The reconstructed and refined central carbon metabolism network comprises 31 reactions (excluding the efflux and influx reactions) and 40 metabolites in total. An elementary flux mode analysis of central carbon metabolism was carried out to investigate the underlying biochemical mechanism of hydrogen production by determining their intermediate fluxes, using linear programming (LP) method available in CellNetAnalyzer software. An in silico gene knockout of lactate dehydrogenase (ldh) and pyruvate kinase (pyk) of the modeled central carbon metabolism pathway allows the maximum theoretical hydrogen yield of 46.2758 mmol/gDW/hr. The single gene knockout of ldh gene tends to increase the flux rate of hydrogen to 32%. Similarly, double gene knockout of ldh and pyk genes yielded an increase in hydrogen flux rate to 35%. Ultimately, this elementary flux mode analysis study of central carbon metabolism pathway of C. saccharolyticus with glucose as substrate or carbon source would further act as a model for strain improvement.

Key-Words / Index Term :
Caldicellulosiruptor saccharolyticus; Biohydrogen; pathway reconstruction; elementary modes

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