Assessment of Probiotic Potential Leuconostoc Mesenteroides from Natural Microbiota

Sathyapriya A.¹ , Anitha A.²

Section:Research Paper, Product Type: Isroset-Journal
Vol.5 , Issue.6 , pp.26-33, Dec-2018

CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v5i6.2633

Online published on Jan 01, 2018

Copyright © Sathyapriya A., Anitha A. . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
A Lactic Acid Bacterium (LAB) is a group of Gram-positive, non-spore forming, motile microorganisms, highly in fermented foods and are well known for their probiotic properties. A heterofermentative lactic acid bacterium, primarily of Leuconostoc mesenteroides from the natural microbiota (sauerkraut fermentation) was isolated, and identified by conventional biochemical characteristics and 16S rDNA gene sequence analysis. Based on the above the isolated natural microbiota was identified as Leuconostoc mesenteroides and deposited in MCC for general access (MCC 3276). The isolated strain had the ability of tolerate bile salts and expressed resistance towards low pH. The ability of bacterial isolates showed autoaggregation and coaggregation which is directly related it adherence capability into the intestinal wall which might have competed with undesirable microorganism in the gut.The tolerance to various biological barriers such as lysozyme (100µg), gastric juice (pH3.0) and bile salt (0.5%w/v) were confirmed its ability to survive extreme conditions of digestive tract. L. mesenteroides has no clear transparent zone in blood agar plates and thus were found non-hemolytic. Overall results indicated that the isolate, Leuconostoc mesenteroides, might be an attractive candidate for perspectival strain for probiotics.

Key-Words / Index Term :
Sauerkraut fermentation, Leuconostoc mesenteroides, Biological characteristic, Molecular identification, Probiotics

References :
[2]. K. G. Deshpande, C.B. Dolas, N.S. Chavan, “Investigation of tolerance of acids, bile salts and deconjugation of bile salts”, International journal of current Microbiology and Applied sciences, Vol. 3, Issue. 7, pp. 600-612, 2014.
[3]. Carlos Ricardo Soccol, Luciana Porto de Souza Vandenberghe, Michele Rigon Spier,Adriane Bianchi Pedroni Medeiros, Caroline TiemiYamaguishi, Juliano De Dea Lindner, Ashok Pandey and Vanete Thomaz- Soccol, “The Potential of Probiotics”,Food Technology and Biotechnology ,Vol. 48 ,Issue.4,pp. 413–434,2010.
[4]. RishikeshShukla, Ilia Ilie, &Arun Goyal, “Leuconostoc mesenteroides NRRL B-1149 as probiotic and its dextran with anticancer properties”, Journal of Bioscience and Biotechnology, Vol.3,Issue. 1, pp.79-87,2014.
[5]. Gordana R Dimic, “Characteristics of the Leuconostoc mesenteroides. Subsp.Mensenteroidesstrain from fresh vegetables”, APTIFF, Vol. 37, pp. 3-11, 2006.
[6]. Wei Zhang, Mingqi Liu, Xianjun Dai, “Biological characteristics and probiotic effect of Leuconostoc lactis strain isolated from the intestine of black porgy fish”,Brazilian Journal of Microbiology, Vol. 44, Issue. 3, pp.685-691, 2013.
[7]. Suzanne Johanningsmeier, Roger, F. Mcfeeters, P. Henry, Fleming and L.Roger, “Thompson effects of Leuconostoc mesenteroides starter culture on fermentation of cabbage with reduced salt concentrations”, Journal Food Science, Vol. 72, Issue. 5, pp.166 – 172, 2007.
[8]. M. Tamminen, T. Joutsjoki, M. Sjoblom, M. Joutsen, A. Palva, E.L. Ryhanen, and V. Joutsjoki,“Screening of lactic acid bacteria from fermented vegetables by carbohydrate profiling and PCR–ELISA”,Letter of Applied Microbiology, Vol. 39, pp.439–444, 2004.
[9]. P. Baumann, and R.H.W. Schubert, “Vibrio naceaeVeron”,Bergey’s manual of systematic bacteriology,pp.516–550, 1984.
[10]. S. K. Allameh, Daud, H. Yusoff, F. M. Saad, C. R. and A. Ideris, A, “Isolation, identification and characterization of Leuconostocmesenteroidesas a new probiotic from intestine of snakehead fish (ChannaStriatus)”, African Journal of Biotechnology, Vol. 11, Issue. 16, pp-.3810–3816, 2012.
[11]. B. Sreelakshmi, “Molecular approaches for characterization and Determination of Pathogenecity of vibrios with special reference to vibrio harveyi from penaeusmondon larval production system”, Ph.D Thesis, 2011.
[12]. R.C. Edgar, “MUSCLE: multiple sequence alignment with high accuracy and high throughput”, Nucleic Acids Res, Vol. 32, Issue. 5, pp.1792-1797, 2004.
[13]. G. Talavera, and J. Castresana, “Improvement of phylogenies after removingdivergent and ambiguously aligned blocks from protein sequence alignments”,Systematic Biology, Vol. 56, pp. 564-577, 2007.
[14]. A. Dereeper, V. Guignon, G. Blanc, S. Audic, S. Buffet, F. Chevenet, J.F. Dufayard, S. Guindon,V. Lefort, M. Lescot, J.M. Claverie, O.Gascuel, “Phylogeny.fr: robust phylogeneticanalysis for the non-specialist”,Nucleic Acids Res. Vol. 1, pp.36, 2008.
[15]. T.C. Clarke, L.I. Black, B.J. Stussman, P.M. Barnes, R.L. Nahin, “Trends in the use of complementary health approaches among adults: United States, 2002–2012”, Natl. Health Stat. Rep, Vol. 79, pp.1–16, 2015.
[16]. V. Bell, J. Ferrão, T. Fernandes, “Nutritional Guidelines and Fermented Food Frameworks”, Foods, Vol. 6, pp.65. [CrossRef] [PubMed] ,2017.
[17]. R. Karthik, C. Angelin, Pushpam. and M.C. Vanitha, “Development of Marine derived probiotic bacterial consortium for the sustainable management of Litopenaeusvannameiculture”, Int. J. Advanced Research in Engineering and Tech, Vol. 6, pp. 62-75, 2015.
[18]. B. Del Re, B.Sgorbati, M. Miglioli , D.Palenzona, “Adhesion, autoaggregation and hydrophobicity of 13 strains of Bifidobacteriumlongum”,Lett. Appl. Microbiol, Vol. 31, pp. 438-442, 2000.
[19]. B. Kos, J. Suskovic, S. Vukovic, M. Simpraga, J. Frece, S. Matosic, “Adhesion and aggregation ability of probiotic strain Lactobacillus acidophilus M92,J. Appl. Microbiol, Vol. 94, pp. 981-987, 2003.
[20]. M. Zago, M.E. Fornasari, D. Carminati, P. Burns, V. Suàrez, G.Vinderola, J. Reinheimer,G. Giraffa G, “Characterization and probiotic potential of Lactobacillus plantarumstrains isolated from cheeses”, Food Microbiol, Vol.28, pp.1033-140, 2011.
[21]. A.H. Rickard, P. Gilbert, N.J. High, P.E. Kolenbrander, P.S. Handley,“An integral process in the development of multi-species biofilms”, Trends Microbiol Bacterial coaggregation, Vol.11,pp.94-100, 2003.
[22]. A. Cebeci, C. Gurakan, “Properties of potensial probiotic Lactobacillus plantarumstrains”, Food Microbiol., Vol.20, Issue. 20, pp. 511-518, 2003.
[23]. T.D.T. Nguyen, J.H. Kang,M.S Lee, “Characterization of Lactobacillus plantarumPH04, a potential probiotic bacterium with cholesterol-lowering effects”, Int.J. Food Microbiol,Vol. 113, pp.358-361, 2007.
[24]. M. Begley, C.G.M. Gahan, Hill, “The interaction between bacteria and bile”, FEMS Microbiol. Rev, Vol. 29,pp-.625-651, 2005.
[25]. S. Salminen, S, Wright, A.Ouwehand, Lactic Acid Bacteria (1). New York: Marcel Dekker, Inc, 2004.
[26]. S. Erkkilä, E. Petäjä, “Screening of commercial meat starter cultures at low pH and in the presence of bile salts for potential probiotic use”, Meat Sci, Vol. 55, pp. 297-300, 2000.
[27]. D.H. Kim, B. Austin, “Characterization of probiotic carnobacteria isolated from rainbow trout (Oncorhynchusmykiss) intestine”, Lett. Appl. Microbiol, Vol. 47, Issue. 3, pp.141-147, 2008.
[28]. A.A. Argyri, G. Zoumpopoulou, G. Kimon-Andreas, G, “Selection of potential probiotic lactic acid bacteria from fermented olives by in vitro tests”,Food Microbiology, Vol.33,Issue.1, pp.282–291, 2013.
[29]. FAO/WHO,“Report on Drafting Guidelines for the Evaluation of Probiotics in Food”, FAO/WHO. Joint FAO/WHO Working Group London, Canada, 2002.