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Bacterial Production of Protease-A Preliminary Study

R. Anusuya1 , K. Srimathi2

Section:Research Paper, Product Type: Isroset-Journal
Vol.5 , Issue.6 , pp.163-169, Dec-2018


CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v5i6.163169


Online published on Jan 01, 2018


Copyright © R. Anusuya, K. Srimathi . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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IEEE Style Citation: R. Anusuya, K. Srimathi, “Bacterial Production of Protease-A Preliminary Study,” International Journal of Scientific Research in Biological Sciences, Vol.5, Issue.6, pp.163-169, 2018.

MLA Style Citation: R. Anusuya, K. Srimathi "Bacterial Production of Protease-A Preliminary Study." International Journal of Scientific Research in Biological Sciences 5.6 (2018): 163-169.

APA Style Citation: R. Anusuya, K. Srimathi, (2018). Bacterial Production of Protease-A Preliminary Study. International Journal of Scientific Research in Biological Sciences, 5(6), 163-169.

BibTex Style Citation:
@article{Anusuya_2018,
author = {R. Anusuya, K. Srimathi},
title = {Bacterial Production of Protease-A Preliminary Study},
journal = {International Journal of Scientific Research in Biological Sciences},
issue_date = {12 2018},
volume = {5},
Issue = {6},
month = {12},
year = {2018},
issn = {2347-2693},
pages = {163-169},
url = {https://www.isroset.org/journal/IJSRBS/full_paper_view.php?paper_id=960},
doi = {https://doi.org/10.26438/ijcse/v5i6.163169}
publisher = {IJCSE, Indore, INDIA},
}

RIS Style Citation:
TY - JOUR
DO = {https://doi.org/10.26438/ijcse/v5i6.163169}
UR - https://www.isroset.org/journal/IJSRBS/full_paper_view.php?paper_id=960
TI - Bacterial Production of Protease-A Preliminary Study
T2 - International Journal of Scientific Research in Biological Sciences
AU - R. Anusuya, K. Srimathi
PY - 2018
DA - 2019/01/01
PB - IJCSE, Indore, INDIA
SP - 163-169
IS - 6
VL - 5
SN - 2347-2693
ER -

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Abstract :
Proteolytic enzyme, also called protease, proteinase, or peptidase, any of a group of enzymes that break the long chainlike molecules of protein into shorter fragments (peptides) and eventually into their components, amino acids proteolytic enzymes are present in bacteria. Protease are produced from various sources but only little information is available regarding the bacterial population in fish wastes of Chidambaram fish market and their efficiency in synthesizing protease enzyme. Isolated 25 bacterial strains and found that only 5 of them are capable of producing protease enzyme that are Pseudomonas sp. and Bacillus sp. was the best protease producer among the tested strains. In the present study, all the bacterial species isolated had a better potentiality of degrading market fish wastes.

Key-Words / Index Term :
Protease, Enzyme, Pseudomonas and Bacillus

References :
[1]. Abidi,F., F.Limam, M. Marzouki and Neji, 2008, Production of alkaline protease by Botrytis cinerea using economic raw materials : Assay as biodetergent. Process.Biochem. 43:1202-1208.
[2]. Adinarayana, K and P. Ellaiah, 2002. Response surface optimization of critical medium components for the production of alkaline protease by a newly isolated Bacillus sp. J.Pharm.Sci., J5:272-276.
[3]. Adinarayana, K., P. Ellaiah, and D.S. Prasad, 2003. Purification and partial characterization of thermostable serine alkaline protease from a newly isolated Bacillus subtilis PE-11, AAPS Pharm. Sci. Tech., 4:56pp.
[4]. Afify, A.E.M.R., M.A.M. Aboul-soud, M.S.Foda,M.W.A.Sadik, T. Kahil,A.R.Asar and A.A.A1-Khedhairy,2009. Production of alkaline protease and larvicidal biopesticides by an Egyptian Bacillus sphaericus isolate. African J.Biotechnol., 8:3864 – 3873.
[5]. Annamalai, N.,M.V. Rajeswari and T.Balasubramanian, 2013. Extraction, purification and applicaton of thermostable and halostable alkaline protease from Bacillus alveayuensis CAS 5 using marine waste. Food and products processing. Doi.org/10.1016/j.fbp.2013.08.009.
[6]. Annamalai, N., M.V. Rajeswari, Sunil Kumar Sahu and T.Balasubramanian, 2014. Purification and characterisation of solvent stable, alkaline proteasefrom Bacillus firmus CAs 7 by microbial conversion of marine wastes and molecular mechanism underlying solvent stability. Process Biochem., http;//dx.doi.org/10.1016/j.2013.08.009.
[7]. Arulmani, M., K.Aparanjini, K. Vasanthi, P. Arumugam, M.Arivuchelvi and T. Kalaichelvan, 2006. Purification and partial charecterisation of serine protease from thermostable alkalophilic Bacillus laterosporus-AK1. World J.Microbiol.Biotechnol., 23; 475-481.
[8]. Ashok Pandey, Carlos R. Soccol and Vanete T. Soccol, 2000. Biopotential of immobilized amylases: a review. Indian J.Microbial., 40:1-14.
[9]. Beg. Q.K. and R. Gupta, 2003. Purification and characterization of an oxidation. Stable, thiol-dependent serine alkaline protease from Bacillus mojavensis. Enzyme. Microb. Technol., 32: 294-304.
[10]. Chang, 2002. Optimization of the production of and extracellular alkaline protease from Bacillus horikoshii. Process Biochem., 38, 155-159.
[11]. Chen, X. G., O. Stabnikova, J.H. Tay, J. Y. Wang and S.T. Lee Tay, 2004. Thermo active xtracellular protease of Geobacillus caldoproteolyticus.,nov., from sewage sludge. Extremophiles. 8: 489-498.
[12]. Cheong, J.Y., N.A.A. Aziz, R. Go, N.U.Azmi,N.S.A. Ghafar, A.A. Adli and M. Mustafa, 2014. The availability of astaxanthin from shrimp shell wastes through microbial fermentations, Aeromonas hydrophila and cell disruptions. Int. J.Agric.Bio., 16: 277-284.
[13]. Chu, I.M., C.Lee and T.S.Li, 1992. Production and degradation of alkaline protease in batch cultures of Bacillus subtilis. ATCC 14416. Enzym. Microb. Technol., 4.55-61.
[14]. Deng, A.,J. Wu, Y. Zhang, G. Zhang, and T. Wen, 2010. Purification and characterization of a surfactant stable high alkaline protease from Bacillus sp. B001. Bioresour. Technol., 101: 7100-7106.
[15]. Dutta, A., J. Wu, Y.Zhang, G. Zhang, and T. Wen, 2010. Isolation and characterization of newly isolated Pseudomonas mutant for protease production. Brazilian Arch. Biol. Technol., 49 (1): 37-47.
[16]. Esakkiraj, P., G. Immanuel, S.M. Sowmya, P. Iyapparaj and A. Palavesam, 2008. Evaluation of protease producing ability of fish gut isolate Bacillus cereus for aqua feed. Food Biopro. Technol., DOI 10. 1007/s 11947-007-0046-6.
[17]. Gessesse, A., R.H. Kaul, B.A. Gashe and B. Mattiasson, 2003. Novel alkaline protease from alkaliphilic bacteria grown on chicken feather. Enzyme Microb. Technol., 32: 519-524.
[18]. Heidari, H.R. K., M.A. Amoozegar, M. Hajighasemi, A.A. Ziaee and A. Ventosa, 2009. Production, optimization and purification of a novel extracellular protease from the moderately halophilic bacterium Halobacillus Karajensis, J. Ind. Microbial. Biotechnol., 35: 121-131.
[19]. Hossain, M.T., H.M. Cherry and M.N. Anwar, 2005. Optimization of some factors affecting the production of glucoamylase by Aspergillus funiculosus. Indian J. Microbiol., 45(2): 143-146.
[20]. Joo, H.S., C. Ganesh Kumar, G.C. Park, K.T. Kim, S. R. Paik and C.S. Kumar, D. and T.C. Bhalla, 2004. Purification and characterization of small size protease from Bacillus sp., APR-4. Indian J. Exp. Biol. 42. 515-521.
[21]. Manivannan, S., D. Nagavally and T. Ramanathan, 2004. Alkaline protease production and assay parameters optimization in estuary isolate of Aspergillus sp. Indian J. Appl. Microbiol. 4(1): 31-35.
[22]. Meenu, M., S. Dhillon and R. Singh, 2000. Production of alkaline protease by a UV-mutant of Bacillus polymyxa. Indian J. Microbiol., 40: 25-28.
[23]. Nawani, N. N., D. Prakash and B.P. Kapadnis, 2010. Extraction, purification and characterization of an antioxidant from marine waste using protease chitinase cocktail. World J. Microbiol. Biotechnol., 26: 1509-1517.
[24]. Okonko, I.O., O.P. Olabode and O.S. Okeleji, 2006. The role of biotechnology in the socio-economic advancement and national development: An overview. African J. Biotechnol., 5(19): 2354-2366.
[25]. Pandey, A., C.R. Soccol and D.Mitchell, 2000. New developments in solid-state fermentation. I: Bioprocesses and applications. Process Biochem., 35: 1153-1169.
[26]. Sabir, J. S. M. and E.E. Bestawy, 2009. Enhancement of alkaline protease production in Bacillus circulans using plasmid transformation. World. J Microbiol. Biotechnol., 25 (11) : 2021-2027.
[27]. Sachindra, N. M., N. Bhaskar and N.S. Mahendrakar, 2005. Carotenoids in different body components of Indian shrimps. J. Sci. Food Agric., 85:167-172.
[28]. Saxena, S.., J. Bahadur and A. Varma, 1993. Cellulose and hemicellulose degradation bacteria from termite gut and mound soils of India. Int. J. Microbiol., 33(1): 55-60.
[29]. Venugopal, M. and A. V. Saramma, 2006. Characteriazation of alkaline protease from Vibrio fluvialis strain VM10 isolated from a mangrove sediment sample and its application as laundry detergent additive. Process Biochem., 41: 1239-1243.
[30]. Wang. S. L., C.H. Yang, T. W. Liang and Y.H. Yen, 2008. Optimization of conditions for protease production by Chryseobacterium taeanense TKU001. Bioresour. Technol., 99:3700-3707.
[31]. Wang, S. L., Chia-Hsing, Chao, T.W.Liang and C.C. Chen, 2009. Purification and characterization of protease and chitinase from Bacillus cereus TKU006 and conversion of marine wastes by these enzymes. Mar. Biotechnol., 11: 334-344.

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