Anticonvulsant Activity of Majoon Najah Against PTZ Induced Seizure in Swiss Albino Mice

Zeba Afrin¹ , Aisha Siddiqui² , M.A Jafri³ , Divya Vohora⁴ , M Asif⁵

Section:Research Paper, Product Type: Isroset-Journal
Vol.5 , Issue.6 , pp.170-179, Dec-2018

CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v5i6.170179

Online published on Jan 01, 2018

Copyright © Zeba Afrin, Aisha Siddiqui, M.A Jafri, Divya Vohora, M Asif . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
Objective: To compare the anticonvulsant activity of Majoon Najah, its hydroalcoholc extract and granular form through Pentylenetetrazole (PTZ) test in swiss albino mice. Material & Methods: Majoon Najah (MN), its hydroalcoholic extract (HEMN) and granular form (GMN) were tested for anticonvulsant activity using Pentylenetetrazole (PTZ) test animal model. To evaluate the effect of test drugs on motor co-ordination, muscle co-ordination test by rotarod was carried out. Results: It was found that MN (p<0.01), HEMN and GMN (p<0.001) significantly increased the latency of myoclonic jerks and clonic generalized seizures when compared with control. The findings of the rotarod test suggest that the test drugs (MN, HEMN and GMN) did not produced any motor inco-ordination. So MN, HEMN and GMN are safer than conventional antiepileptic drugs. Conclusion: It can be concluded from the experiment performed that Majoon Najah, its hydoalcoholic extract and granular form possess significant anticonvulsant activity.

Key-Words / Index Term :
Majoon Najah, anticonvulsant, pentylenetetrazole, mice

References :
[1]. T. A Dua, “Neurological Disorders: public health challenges”, World Health Organization, Geneva, 2006.
[2]. Anonymous, “Executive Summary. In Atlas: Epilepsy Care in the world”, World Health Organization, Geneva, pp.3, 2005.
[3]. R. Sridharan, “Epidemiology of Epilepsy”, Current Science, Vol. 82, Issue 6, pp.664-670, 2002.
[4]. H.H. Merritt & L.P. Rowland, “Merritt`s Neurology (10th Edition)”, Lippincott Williams & Wilkins Publishers, 2000.
[5]. M.K. Azam, “Al-Akseer (Urdu Translation by Kabeeruddin HM)”, Aijaz Publishing House, New Delhi, pp.151-164, 2010.
[6]. A. Wahab, “Difficulties in Treatment and Management of Epilepsy and Challenges in New Drug Development”, Pharmaceuticals, Vol. 3, pp.2090-2110, 2010.
[7]. V. Kamboj, “Herbal medicine”, Current Science, Vol. 78, Issue 3, pp. 35-39, 2000.
[8]. R.K. Malvi, B. Papiya, S. Sunny & S. Jain, “Medicinal plants used in the treatment of epilepsy”, International Research Journal of Pharmacy, Vol. 2, Issue 2, pp.32-39, 2011.
[9]. J.A. Hassan, “Zakheera Khwarzam Shahi”, Vol. II, pp.196-198, YNM.
[10]. Anonymous, “National Formulary of Unani Medicine”, Part I, Ministry of Health & Family Welfare (Department of AYUSH), Govt. of India, New Delhi, pp.138, 2006.
[11]. Anonymous, “The Useful plants of India”, Publications & Information Directorate, CSIR, New Delhi, pp.152,319,320,409, 1986.
[12]. S.S. Chaudhary, “Solid dosage forms in unani ssytem of medicine”, Journal of Pharmaceutical and Scientific Innovation, Vol. 2, Issue. 3, pp.17-22, 2013.
[13]. Anonymous, “NFUM, Part II, Vol. I”, CCRUM, Ministry of Health & Family Welfare, New Delhi, pp. 100,192, 2007.
[14]. D. Z. Antaki, “Tadhkiratu Ulil Albaab Wal Jame Lil Ajabil Ujaab”, Part I, Al-Matba Atul Aamiratus Saqafiyah, Misr, 1899.
[15]. G.M. Choghtai & F. Choghtai, “Bayaz-e-Ferozi”, Sheikh Muhammad Basheer & Sons, Lahore, pp.163, YNM.
[16]. A. R. Gennaro, “Remington: The Science and Practice of Pharmacy”, Vol. I (20th Edition), Lippincott Williams & Wilkins, pp. 36, YNM.
[17]. Anonymous, “Guidance for industry estimating the maximum safe starting dose in initial clinical trials for therpaeutics in adult healthy volunteers”, Department of Health and Human Services, Food and Drug Administration, Center for Drug Evaluation and Research, U.S., pp. 6-7, 2005.
[18]. D. Vohora, S. Pal & K. Pillai, “Thioperamide, A selective Histamine H3 receptor antagonist, protects against PTZ-induced seizures in mice”, Pharmacology letters, Life Sciences, Vol.66, Issue.22, pp. 297-301, 2000.
[19]. R. Joshi, K. Reeta, S. Sharma, M. Tripathi & Y. Gupta, “Panchagavya Ghrita, an Ayurvedic formulation attenuates seizures, cognitive impairement and oxidative stress in pentylenetetrazole induced seizure in rats”, Indian Journal of Experimental Biology, Vol.53, pp. 446-451, 2015.
[20]. G. Radha & A. Goel, “Effect of Nebivolol on Anticonvulsant Property of Sodium Valproate along with Neuropharmacological benefits in Rodents Models of Epilepsy and Behaviour in Mice”, Journal of Clinical and Experimental Neuroscience, Vol.1, Issue.1, 2013.
[21]. A. M. Z Razi, “Kitabul Havi (Urdu Translation by CCRUM)”, Vol. I, Ministry of Health & Family Welfare, New Delhi, pp. 106-130,1997.
[22]. H.-Y. Cheng, T.-C. Lin, K.-H. Yu, C.-M. Yang, & C.-C Lin, “Antioxidant and Free Radical Scavenging Activities of Terminalia chebula”, Biol. Pharm. Bull, Vol. 26, Issue.9, pp. 1331-1335, 2003.
[23]. Senthil G. K, & Subramanian S. Evaluation of Antioxidant Potential of Terminalia chebula fruits studied in Streptozotocin-Induced Diabetic Rats. Pharmaceutical Biology. 2007; 45(6): 511-518.
[24]. Shekhar C. R., Manohar V., & Rao S. Antidepressant activity of aqueous extracts of fruits of Terminalia chebula in rats. International Journal of Pharmacy and Pharmaceutical Sciences. 2012; 4(4): 449-451.
[25]. C. L Chang & C. S. Lin, “Phytochemical Composition, Antioxidant Activity and Neuroprotective Effect of Terminalia chebula Retz Extracts”, Evidence-Based Complementary and Alternative Medicine, pp. 1-7, 2012.
[26]. J. Debnath, U. R. Sharma, B. Kumar & N. S. Chauhan, “Anticonvulsant activity of ethanolic extracts of Terminalia chebula on experimental animals”, International Journal of Drug Development & Research, Vol.2, Issue.4, pp. 764-768, 2010.
[27]. B. Hazra, R. Sarkar, S. Biswas & N. Mandalnn, “Comparative study of the antioxidant and reactive oxygen species scavenging properties in the extracts of the fruits of T.chebula, T.bellerica and E.officinalis” BMC, Complementary and Alternative Medicine, pp.1-15, 2010.
[28]. S. R. Chandra, V. Manohar, P. Bharathi & M. Rai, “Attenuation of anxiety on acute administration of aqueous extract of Terminalia belerica fruit pulp in Swiss albino mice” International Journal of Basic & Clinical Pharmacology, Vol.6, Issue.2, pp. 303-307, 2017.
[29]. M. Golechha, M. Bhatia, A. Jagriti, & D. Singh, “Hydroalcoholic extract of Emblica officinalis Gaertn. affords protection against PTZ induced seizures, oxidative stress and cognitive impairement in rats”, Indian Journal of experimental Biology, Vol. 48, pp. 474-478, 2010.
[30]. S. Khopde, K. I. Priyadarshani, H. Mohan, V. Gawandi, J. Satav, J. Yakhmi, “Characterizing the antioxidant activity of amla (Phyllanthus emblica) extract”, Current Science, Vol.81, Issue.2, pp. 185-190, 2001.
[31]. C. Anbuselvam, K. Vijayavel & M. Balsubramaniyan, “Protective effect of Operculina turpethum against 7,12 dimethylbenz(a)anthracene induced oxidative stress with reference to breast cancer in experimental rats”, Chemico Biological Interactions, Vol. 168, pp. 229-236, 2007.
[32]. P. A. Dar, G. Sofi & M. Jafri, “Polypodium vugare Linn. A Versatile Herbal medicine: A Review”, IJPSR, Vol.3, Issue.6, pp. 1616-1620, 2012.
[33]. S. Yadav, V. Tripathi, R. Singh, & H. Pandey, “Antioxidant activity of Cuscuta reflexa stems”, Indian J. Pharm Sci, Vol. 62, Issue.6, pp. 477-478, 2000.
[34]. S. Thomas, S. Srikumar, C. Velmurugan & A. B. Kumar, “Evaluation of anxioltic effect of whole plant of “Cuscuta reflexa”, World Journal of Pharmacy and Pharmaceutical Sciences, Vol.4, Issue.8, pp. 1245-1253, 2015.
[35]. H. Sebai, S. Selmi, K. Tibi, A. A. Souli, N. Gharbi & M. Sakly, “Lavender (Lavandula stoechas L.) essential oils attenuate hyperglycemia and protect against oxidative stress in alloxan-induced diabetic rats”. Lipids in Health and Disease, pp. 1-9, 2013.
[36]. S. Miraj, “Lavandula stoechas L: A systematic review of medicinal and molecular perspectives”, Der Pharmacia Lettre, Vol.8, Issue.13, pp. 56-58, 2016.
[37]. W. Loshcher & D. Schmidt, “Which animal models should be used in the search for new antiepileptic drugs? A proposal based on experimental and clinical considerations”, Epilepsy Research, Vol.2, pp. 145-181, 1988.
[38]. S. Mehrzadi, A. Shojaii, S. A. Pur & M. Motevalian, “Anticonvulsant Activity of Hydroalcoholic Extract of Citrullus colocynthis Fruit:Involvement of Benzodiazepine and Opioid Receptors”, Journal of Evidence-Based Complementary & Alternative Medicine, Vol.21, Issue.4, pp. 31-35, 2015.