Siderophores detection by using blue agar CAS assay methods

K. Srimathi¹ , H. Ann Suji²

Section:Research Paper, Product Type: Isroset-Journal
Vol.5 , Issue.6 , pp.180-185, Dec-2018

CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v5i6.180185

Online published on Jan 01, 2018

Copyright © K. Srimathi, H. Ann Suji . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
The low iron molecules produced from various sources are the siderophores. These are taken up by bacteria to help their process of iron uptake which is necessary for all microbes to obtain environmental iron indeed for important processes. In the present study, siderophore production by bacteria present in the coastal waters of Parangipettai was analyzed. Among the total of 40 isolates obtained, only five were able to produce siderophores by the CAS agar plate assay method. The production of an orange or a yellow hallow zone around a colony indicated that it is a siderophore producer. The present study provides a detailed protocol to prepare the CAS agar without any difficulty.

Key-Words / Index Term :
Siderophores, ferric iron, Bacillus, Pseudomonas

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