Biodegradation of Organo phosphorous Pesticide Dichlorvos by bacteria isolated from field sample

Ravi Pandey¹ , Y. Aparna²

Section:Research Paper, Product Type: Journal Paper
Vol.06 , Special Issue.01 , pp.41-45, May-2019

CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v6si1.4145

Online published on May 10, 2019

Copyright © Ravi Pandey, Y. Aparna . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.  

View this paper at   Google Scholar | DPI Digital Library

PDF Download

How to Cite this Paper

Abstract :
The extensive use of pesticides is considered to be one of the major causes of soil and water pollution in agricultural sector. As it enters the food chain, humans on the other hand are directly or indirectly victimized. In order to counteract the situation, such contaminants need to be removed from the environment. The safest approach to clean the environmental pollution in an eco-friendly way is by employing microorganisms, a process termed as biodegradation. Dichlorvos is an organophosphorus pesticide widely used as insecticide to control pest in agriculture.In the present study an attempt was made to screen for potential Dichlorvos degrading bacteria. The soils collected from various field samples are subjected to enrichment culture technique using various concentrations of pesticides in minimal salt medium. Of all the organisms screened only four isolates labelled as SA, SB, SC and SD showed degradative activity. Organophosphate enzyme assay was performed to confirm the degradation by using UV-Visible spectrophotometer. However further studies are to be done to check whether the cultures are showing complete degradation, partial degradation or bioconversion.

Key-Words / Index Term :
Biodegradation, Dichlorvos, Organophosphate assay, Organophosphorus pesticide

References :
[1]. Shaohua Chen, Chenglan Liu, Chuyan Peng, Hongmei Liu, Meiying Hu, Guohua Zhong. Biodegradation of chlorpyrifos and its hydrolysis product 3,5,6-trichloro-2-pyridinol by a new fungal strain Cladosporium cladosporioides Hu-01. PloS One, 7: 47205, 2012
[2]. Singhal, V, “Indian Agriculture 2003”.Indian Economic Data Research Centre, New Delhi, pp.85-93, 2003
[3]. Hazra, C.R., Demand pattern of pesticides in India. Pesticide Information, 26: 41-45, 2001.
[4]. Tatheer Alam Naqvi1, Nisar Ahmed Kanhar ,Abdul Hussain Shar ,Masroor Hussain and Safia Ahmed. Microcosm studies for the biodegradation of carbaryl in soil. Pak. J. Bot., 43(2): 1079-1084, 2011.
[5]. Natala, A.J. and Ochoje, O.S. Survey of pesticides used in the control of ectoparasites on farm animals in Kaduna State, Northern Nigeria. J. Animal and Plant Sci. 4 (1): 276 – 280, 2009.
[6]. Nsikak U. B. and Aruwajoye I. O. Assessment of contamination by organochlorine pesticides in Solanum lycopersicum L. and Capsicum annuum L.: A market survey in Nigeria. Afri.J. Environ.Sci.Technol.. 5(6): 437-442, 2011.
[7]. Kanekar, P.P., Bhadbhade, B., Deshpande, N.M. and Sarnaik, S.S., Biodegradation of organophosphorus pesticides. Proceedings of Indian National Science Academy, 70: 57-70, 2004.
[8]. Bakry NM, el-Rashidy AH,Eldefrawi AT,Eldefrawi ME.Direct actions of organophosphate anticholinesterases on nicotinic and muscarinic acetylcholinic receptors. J. Biochem. Toxicol. 3: 235-259,1988
[9]. Serdar, C.M and Gibson, D.T. Enzymatic hydrolysis of organophosphates cloning and expression of a parathion hydrolase gene from Pseudomonas diminuta. Biotechnol. 3: 567-571, 1985.
[10]. Grimsley, J., Rastogi, V. and Wild, J.. Biological detoxification of organophosphorus neurotoxins. In: Bioremediation Principles and Practice-Biodegradation Technology Developments (S. Sikdar and R. Irvine, Eds). Technomic Publishers, New York. pp 557-613, 1998.
[11]. Mohammed, M.S. Degradation of methomyl by the novel bacterial strain Stenotrophomonas maltophilia MI. Electronic J. Biotechnol. 12 (4): 1- 6, 2009.
[12]. Mark R McCoy,Zheng Yang,Xun Fu,Ki Chang Ahn, Shirley J.Gee,David C Bom,Ping Zhong, Dan Chang and Bruce D.Hammock.Monitoring of total type II pyrethroid pesticides in citrus oils and water by converting to a common product 3-phenoxybenzoic acid. J. Agric. Food Chem., 60: 5065-5070,2012
[13]. Yonar SM, Ural MS, Silici S, Yonar ME. Malathion-induced changes in the haematological profile, the immune response and the oxidative/antioxidant status of Cyprinus carpiocarpio: Protective role of propolis. Ecotoxicol. Environ. Safety, 102: 202-209,2014
[14]. S. E. Agarry, O. A. Olu-arotiowa, M. O. Aremu and L. A. Jimoda. Biodegradation of Dichlorovos(Organophosphate pesticide) in soil by bacterial isolates. Journal of Natural sciences Research.Vol 3(8):12-16,2013.
[15]. Soni Yadav, Sitansu Kumar Verma and Hotam Singh Chaudhary. Isolation and characterization of Organophosphate pesticides degrading bacteria from contaminated agricultural soil.Online journalof biological sciences.15(1):113-125,2015
[16]. M A Chandrashekar, M.Supreeth, K Soumya Pai, S K C Ramesh, N Geetha, H R Puttaraju and NS Raju. Biodegradation Of Organophosphorous Pesticide, Chlorpyrifos By Soil Bacterium - Bacillus megaterium RC 88. Asian journal of Microbiology, Biotechnology and Environmental Science paper.Vol 19(1);146-152,2017
[17]. Alvarez-Macarie, E., V. Augier-Magro and J. Baratti. Characterization of a thermostable esterase activity from the moderate thermophile Bacillus licheniformis. Bioscience Biotechnology Biochemistry, 63: 1865-1870,1999.