Screening different microbial flora and their enzymatic activities during tea waste composting

Akhila Godishala¹ , S. Chaitanya Kumari²

Section:Research Paper, Product Type: Journal Paper
Vol.06 , Special Issue.01 , pp.50-59, May-2019

CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v6si1.5059

Online published on May 10, 2019

Copyright © Akhila Godishala, S. Chaitanya Kumari . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.  

View this paper at   Google Scholar | DPI Digital Library

PDF Download

How to Cite this Paper

Abstract :
Proper management of solid waste is a major problem in most of the metropolitan areas. Composting is one of the oldest and simplest methods of organic waste stabilization. Composting tea waste is not only an environmental safe method of its disposal but also enhances the growth of the plants as it contains nutrients and tannic acid which create a more fertile environment. In the present study composting of tea waste with variations in type of soil, type of cattle dung and type of tea leaf waste was studied. From this physical, chemical, biological activities and soil enzymes such as cellulase, pectinase, proteases, amylases, xylanase activities were studied in various combinations like black soil, red soil, tea waste, green tea leaves waste along with cow dung, buffalo dung and goat manure in a ratio of 2:2:1 for a time period of 30 days. Analysis of soil with tea waste and cattle dung revealed that compost soil underwent changes in all measured physicochemical, biological and enzymatic parameters like moisture content recorded as 1.6%, highest temperature as 460 C and percentage of microbial population like bacteria as 60% and fungi as 40% were observed in the compost soil. Higher enzyme activities such as protease, pectinase, xylanase were observed with bacterial isolates comparatively with fungal isolates. PGPR activities such as IAA, Siderophore production, phosphate solubilization and organic acid production were also observed. Improved soil microbial and enzyme activities in cattle dung soil is an indication of improvement in soil fertility. Therefore the produced compost can be used to increase the crop yield and simultaneously decreasing the environmental pollution.

Key-Words / Index Term :
Tea waste, cattle dung, physico-chemical-biological activities, PGPR activities

References :
[1] Pappu A, Saxena M, Asolekar SR. Solid wastes generation in India and their recycling potential in building material. Building and Environment 42(6): 2311-2320,2007.
[2] Dipti yadav, Lepakshi Barbora, Latha Rangan, Pinakeswar Mahanta. Tea waste and food waste as a potential feedstock for biogas production 35(5): 1247-1253 , 2016.
[3] Minakshi Gurav, Smita Sinalkar. Preparation of organic compost using waste tea powder, National conference on biodiversiy : Status and challenges in conservation ISBN : 978-81-923628-1-6, 2013.
[4] Echochem, Innovative solutions for sustainable agriculture in accordance with NOSB (National Organic Standard Board).
[5] R.P.Dick; Soil enzyme activities as indicators of soil quality. In: J.V.Doran, D.C.Coleman, D.F.Bezdick, B.A.Stewrtm (Eds); Defining Soil Qualioty for a sustanaible Environment Soil Science Society of American society of Agriculture, Madison, 107-124 ,1994.
[6] M.A.Tabatabai; Soil enzymes. In: R.W.Weaver, S.Angle, P.Bottomley (Eds); Methods ofsoil analy- sis. Part 2: Microbiological and biochemical prop- erties. Soil Science Society of America, Madison, 775-833,1994.
[7] S.P.Deng, M.A.Tabatabai;Cellulase activity ofsoils. Soil BioBiochem, 26, 1347-1354,1994.
[8] T.W.Speir, D.J.Ross; Phosphorous in action - Biological processes in soil phosphorous cycling, Role of phosphatase enzyme in soil, Soil Biology vol - 26 : 215-245,2001.
[9] J.C.Tarafdar, A.Jungk; Phosphatase activity in the rhizosphere and its relation to the depletion of soil organic phosphorus. Biol.Fert.Soils., 3(4), 199-204,1987.
[10] W.A.Dick, M.A.Tabatabai; Potential uses of soil enzymes. In: F.B.Metting Jr. (Ed); Soil microbial ecology: Applications in agricultural and environ- mental management, Marcel Dekker, New York, 95-127 ,1991.
[11] Diver.S, .Notes on Compost Teas:A Supplement to the ATTRA Publication Compost Teas for Plant Disease Control, ISBN:1-800-346-9140 ,2002 .
[12] ER Ingham, The compost tea brewing manual, Soil food web incorporated, fifth edition 13-91 ,2005.
[13] T. Kanangara, R.C. Durley, G.M. Simpson, High performance liquid chromatographic analysis of cytokinins in sorghum bicolor leaves, physiologia plantrum 44(3):295-299 ,2006.
[14] R Merrill, J McKeon, Apparatus design and experimental protocol for organic compost teas, Organic farming research foundation information bulletin 9,9-15 ,2001.
[15] Taleb Abuzahra, Alaeddin b Tahboub, Effect of Organic Matter sources on chemical properties of the soil and yield of strawberry under organic farming conditions, World Applied Sciences 5(3) 383-388 2008.
[16] Steinegger, D. H. and Janssen, D. E. 1996. Straberries, plants, selecting and preparing a site, planting and care of strawberries. Institute of Agriculture and Natural Resources, University of Nebraska-Lincoln, plant pathol. J. 31(3):259-268, 2015.