Evaluation of Prodigiosin pigment for antimicrobial and insecticidal activities on selected bacterial pathogens & household pests.

B. Sree Vani Sagar¹ , B. Sai Deepak² , G. Sai Tejaswini³ , Y. Aparna⁴ , J. Sarada⁵

Section:Research Paper, Product Type: Journal Paper
Vol.06 , Special Issue.01 , pp.0-0, May-2019

CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v6si1.00

Online published on May 10, 2019

Copyright © B. Sree Vani Sagar, B. Sai Deepak , G. Sai Tejaswini ,Y. Aparna, J. Sarada . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.  

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Abstract :
Bacterial pigments are gaining commercial importance in various industries like food, textile, cosmetics, pharmaceutical and other industries. Bacterial pigments are advantageous over others as they are cost effective and eco friendly. Prodigiosin has been reported to have many applications such as antibacterial, antifungal, tumor suppressor, antimalarial, antiprotozoal, immunosuppressive etc. due to its natural red pigmentation. Applications of prodigiosin prompts one to develop a cost-effective bioprocess for its production. Multi-facilitated potency of prodigiosin pigment produced by Serratia could be explored not only for antibacterial and antifungal but also as effective biocontrol agent in agriculture. Present study therefore aimed to obtain a potential isolate of Serratia for prodigiosin production. Evaluation of the pigment was carried out with four bacterial strains, two fungal plant pathogens and four insect pests. Peanut broth when inoculated with the isolate could yield maximum pigment production at 300C for 72hours. The extracted pigment purity was confirmed by the spectrophotometric method and also by TLC. Prodigiosin was observed to be effective against E.coli and Staphylococcus with an MIC value of 400 µg.However it had no impact on Pseudomonas and Bacillus cultures. When compared with the standard Nistatin, MIC of the pigment was observed as 80 µg. for Alternaria and for Fusarium MIC was observed at 160 µg. Insecticidal activity 100 % mortality was found against Cockroaches and tropical ants while 85 -71% was observed with termites and pyramid ants.

Key-Words / Index Term :
Serratia,anti microbial, antifungal, insecticidal, Prodigiosin

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