Evaluation of medicinal plant extracts and biosynthesized nanoparticles for antibiofilm activity against Pseudomonas biofilm

S. Anju¹ , P. Sai Gayathri² , Sri Charani³ , K.N.S. Sushmitha⁴ , J. Sarada⁵

Section:Research Paper, Product Type: Journal Paper
Vol.06 , Special Issue.01 , pp.0-0, May-2019

CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v6si1.00

Online published on May 10, 2019

Copyright © S. Anju, P. Sai Gayathri, Sri Charani, K.N.S. Sushmitha, J. Sarada . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.  

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Abstract :
Bacterial biofilms are surface attached community of bacteria embedded in a matrix of extracellular polymeric substances. When a cell switches to the biofilm mode ,they undergo a phenotypic shift in behavior to acquire properties which enable their survival under unfavorable conditions and become drug resistant. P. aeruginosa represents a commonly used biofilm model organism since it is involved in different types of biofilm-associated infections like chronic wounds, chronic otitis media, chronic prostatitis and chronic lung infections in cystic fibrosis (CF). A major threat by biofilms is that bacteria in them benefit from enhanced shield against host immune responses and are distressingly more forbearing to various antimicrobial treatments. Treatment of biofilm infections is currently a difficult and complicated challenge for microbiologists and clinicians. Present study evaluates antibiofilm activity of plant extracts and green synthesized nanoparticles as biofilm inhibitory or dispersal agents on Psuedomonas biofilms.Among the extracts investigated ,Ginger ,Amla had maximum antibiofilm activity.However when these two extracts when compared for virulence factor reduction ,only Ginger extract was identified to be effective.Phytochemical analysis revealed that Ginger has quinones,alkaloids & saponins which are proven to be antibacterial agent.Among three metal nanoparticles ZnO nanoparticles were exhibiting antibiofilm activity. Efficacy of ZnO np were further enhanced by fabricating with ginger extract which has good antibacterial and antibiofilm activity.Combinatorial approach of treating drug resistant bacterial biofilms with natural plant extract depicting antibacterial and antibiofilm activity with metal nanoparticles were proven to be more effective .This study gives scope for further research for enhancing the efficacy of metal nanoparticles as antimicrobial agents.

Key-Words / Index Term :
Psuedomonas ,Biofilm inhibition , Biofilm dispersion, Antimicrobial agents, Antibiofilm agents

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