Conjugation of Organic Dyes Onto pH Insensitive Silver Nanoparticles for pH Sensing

J.A. Owusu¹ , K.E. Pobee-Quaynor²

Section:Research Paper, Product Type: Journal-Paper
Vol.6 , Issue.5 , pp.19-25, Dec-2019

Online published on Dec 31, 2019

Copyright © J.A. Owusu, K.E. Pobee-Quaynor . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
pH is a very important parameter in research, production and quality assessment in the fields of life sciences, pharmaceuticals and food industries. Over the years, several methods have been employed to enhance the sensing of pH. This article, however, concentrates on using fluorescence-based nanosensor detection due to its high spatial resolution, fast response, high sensitivity, simple usage and versatility to other detection schemes. Herein, a 2nm size G-AgNPs were synthesized and conjugated to a well-known pH-sensitive dye (NHS-FAM) and a pH insensitive dye (NHS-TAMRA) to produce a fluorescent pH nanoprobe sensor. After conjugating NHS-FAM to G-AgNPs, it showed an increment in fluorescent intensity over a wide range of pH values which led to an R2 value of 0.9992. NHS-TAMRA conjugated onto the as-prepared AgNPs also showed increment in fluorescent intensity over pH values of 5-10 which resulted in linear regression (R2) value of 0.9866

Key-Words / Index Term :
pH, Silver nanoparticles, NHS – 5(6) – carboxytetramethylrhodamine N-succinimidyl ester, 5(6) – carboxytetramethylrhodamine N-succinimidyl ester, Fluorescence, Nanoprobe

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