Effect of Chemical Mutagens on Alpha Amylase Activity of Bacillus Amyloliquefaciens

U.U. Zango¹ , M. Ibrahim² , M. Suleiman³

Section:Research Paper, Product Type: Journal-Paper
Vol.8 , Issue.1 , pp.9-16, Feb-2021

Online published on Feb 28, 2021

Copyright © U.U. Zango, M. Ibrahim, M. Suleiman . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
In this research, the influence of different mutational treatments (Ethidium bromide (EtBr) and acrylamide treatment) on amylase production was studied. EtBr mutant showed increased in alpha amylase activity at 200µg/ml with enzyme activity compared with the wild strain at different incubation time. Increase in EtBr concentration from 200 µg/ml, 300 µg/ml and 400 µg/ml shows increase in enzyme activity 0.603455 U/ml, 0.652753 U/ml and 0.711182 U/ml respectively. Decreased in enzyme activity was observed in EtBr mutant exposed to 500 µg/ml and 600 µg/ml with enzyme activity 0.661883 U/ml and 0.579718 U/ml respectively. Acrylamide mutant showed no increase in enzyme activity at concentration of 200 µg/ml compared with wild strain at different incubation time. Increase in acrylamide concentration from 200 µg/ml, 300 µg/ml and 400 µg/ml shows increase in enzyme activity (0.267492 U/ml, 0.740396 U/ml and 0.818909 U/ml respectively). At concentrations of 500 and 600 the enzyme activity decreased with enzyme activity 0.683793 U/ml and 0.42817 U/ml.

Key-Words / Index Term :
Amylase, acrylamide, bacillus, mutant

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